miR-138-5p inhibits the malignant progression of prostate cancer by targeting FOXC1
Background: This study aimed to uncover the effect of miR-138-5p on the proliferation and metastasis of PCa cell lines, and further explore the potential regulatory mechanisms via regulating FOXC1.
Methods: 60 pairs cancer tissues and corresponding paracancerous ones from PCa patients were collected to assess the expression level of miR-138-5p by qRT-PCR. Subsequently, over-expression of miR-138-5p were established to explore the proliferation and metastasis of miR-138-5p in PCa cell lines was analyzed by CCK-8, Tranwell assay and Wounding healing assay, respectively. Bioinformatics analysis and luciferase reporter gene assay were performed to search for the target genes of miR-138-5p, and FOXC1 was selected. Finally, the biological role of miR-138-5p and FOXC1 in the progression of PCa was clarified by a series of rescue experiments.
Results: The results of qRT-PCR revealed that miR-138-5p was lowly expressed in PCa tissues and cell lines. Besdies, the PCa patients with low-miR-138-5p had a high Gleason score, lymph node metastasis and poor prognosis of PCa, compared with these patients with high-miR-138-5p. Over-expression of miR-138-5p inhibited the proliferative, migratory and invasive capacities of PC-3 and DU-145 cells. Bioinformatics analysis and luciferase reporter gene assay suggested that FOXC1 was predicted to be the target gene of miR-138-5p. Moreover, FOXC1 expression level was negatively correlated to that of miR-138-5p in PCa tissues. Importantly, Over-expression of FOXC1 could reverse miR-138-5p mimic induced-inhibition of PCa malignant progression.
Conclusions: Downregulated miR-138-5p was closely associated with high Gleason score, more lymph node metastasis and poor prognosis of PCa patients. In addition, miR-138-5p alleviated the malignant progression of PCa by targeting and downregulating FOXC1.
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Posted 24 Jun, 2020
On 09 Jul, 2020
On 24 Jun, 2020
On 24 Jun, 2020
On 23 Jun, 2020
On 23 Jun, 2020
On 17 Jun, 2020
Invitations sent on 08 Jun, 2020
On 04 Jun, 2020
On 03 Jun, 2020
On 03 Jun, 2020
On 22 May, 2020
Received 03 May, 2020
On 22 Apr, 2020
Received 09 Apr, 2020
On 21 Mar, 2020
On 09 Mar, 2020
Invitations sent on 09 Mar, 2020
On 08 Mar, 2020
On 04 Mar, 2020
On 03 Mar, 2020
miR-138-5p inhibits the malignant progression of prostate cancer by targeting FOXC1
Posted 24 Jun, 2020
On 09 Jul, 2020
On 24 Jun, 2020
On 24 Jun, 2020
On 23 Jun, 2020
On 23 Jun, 2020
On 17 Jun, 2020
Invitations sent on 08 Jun, 2020
On 04 Jun, 2020
On 03 Jun, 2020
On 03 Jun, 2020
On 22 May, 2020
Received 03 May, 2020
On 22 Apr, 2020
Received 09 Apr, 2020
On 21 Mar, 2020
On 09 Mar, 2020
Invitations sent on 09 Mar, 2020
On 08 Mar, 2020
On 04 Mar, 2020
On 03 Mar, 2020
Background: This study aimed to uncover the effect of miR-138-5p on the proliferation and metastasis of PCa cell lines, and further explore the potential regulatory mechanisms via regulating FOXC1.
Methods: 60 pairs cancer tissues and corresponding paracancerous ones from PCa patients were collected to assess the expression level of miR-138-5p by qRT-PCR. Subsequently, over-expression of miR-138-5p were established to explore the proliferation and metastasis of miR-138-5p in PCa cell lines was analyzed by CCK-8, Tranwell assay and Wounding healing assay, respectively. Bioinformatics analysis and luciferase reporter gene assay were performed to search for the target genes of miR-138-5p, and FOXC1 was selected. Finally, the biological role of miR-138-5p and FOXC1 in the progression of PCa was clarified by a series of rescue experiments.
Results: The results of qRT-PCR revealed that miR-138-5p was lowly expressed in PCa tissues and cell lines. Besdies, the PCa patients with low-miR-138-5p had a high Gleason score, lymph node metastasis and poor prognosis of PCa, compared with these patients with high-miR-138-5p. Over-expression of miR-138-5p inhibited the proliferative, migratory and invasive capacities of PC-3 and DU-145 cells. Bioinformatics analysis and luciferase reporter gene assay suggested that FOXC1 was predicted to be the target gene of miR-138-5p. Moreover, FOXC1 expression level was negatively correlated to that of miR-138-5p in PCa tissues. Importantly, Over-expression of FOXC1 could reverse miR-138-5p mimic induced-inhibition of PCa malignant progression.
Conclusions: Downregulated miR-138-5p was closely associated with high Gleason score, more lymph node metastasis and poor prognosis of PCa patients. In addition, miR-138-5p alleviated the malignant progression of PCa by targeting and downregulating FOXC1.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5