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Research article
vittorio candela
Sapienza University of Rome: Universita degli Studi di Roma La Sapienza
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5962-2598
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The network of intermediate filament proteins underlying the inner nuclear membrane forms the nuclear lamin. A and B-type lamins are the major components of the nuclear lamina. Lamins function in many nuclear activities. The role of lamin-A and transcription factors (NF-kB) as anti-apoptotic is well documented. Recently, lamin-A has also been considered as mechanosensor protein able to maintain nuclear integrity from mechanical insults.
We aimed to verify how lamin-A expression varies in healthy cuff cells and in those with different sized tears where various mechanical stresses are present.
Methods
Forty-three patients with rotator cuff tear (RCT) [23M-20F, mean age (SD): 63.5 (6.1)] were enrolled. Tissue samples excised from the most medial point of tear margins were analyzed for lamin-A expression by immunohistochemistry. Controls were represented by samples obtained by seven healthy supraspinatus tendons excised from patients submitted to reverse shoulder prosthesis implant [3M-4F, mean age (SD): 67.1 (6.3)]. The intensity of staining was graded, and an H-score was assigned. Statistical analysis was performed.
Results
Our study revealed a moderate intensity of lamin-A in the healthy cuff tendons, a higher expression of this protein in the small tears, and a significant decrease of lamin-A with increasing tear size (p<0.0001).
Conclusions
Our study emphasizes the importance of early repair of small RCTs since nuclear stability is maintained, and the cellular function is protected by lamin-A overexpression. High re-tear of massive cuff repair could be due to cellular apoptosis and nuclear modifications induced by lamin-A lack.
Level of Evidence: III
Keywords
Nuclear Lamin A, Mechanostat Factor, Antiapoptotic
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CURRENT STATUS: Under Review
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Posted 08 Feb, 2021
No community comments so far
Editorial decision: Major revision
On 02 Mar, 2021
Review #2 received
Received 24 Feb, 2021
Review #1 received
Received 19 Feb, 2021
Reviewer #4 agreed
On 17 Feb, 2021
Reviewer #3 agreed
On 15 Feb, 2021
Reviewer #2 agreed
On 12 Feb, 2021
Reviews received
Received 12 Feb, 2021
Reviewers invited
Invitations sent on 12 Feb, 2021
Reviewer #1 agreed
On 12 Feb, 2021
Editor assigned
On 27 Jan, 2021
Submission checks complete
On 27 Jan, 2021
Editor invited
On 27 Jan, 2021
First submitted
On 26 Jan, 2021
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Subject Areas
Orthopedic Surgery
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A new preprint design is coming!
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This preprint is under consideration at Journal of Orthopaedic Surgery and Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
vittorio candela
Sapienza University of Rome: Universita degli Studi di Roma La Sapienza
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5962-2598
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 08 Feb, 2021
No community comments so far
Editorial decision: Major revision
On 02 Mar, 2021
Review #2 received
Received 24 Feb, 2021
Review #1 received
Received 19 Feb, 2021
Reviewer #4 agreed
On 17 Feb, 2021
Reviewer #3 agreed
On 15 Feb, 2021
Reviewer #2 agreed
On 12 Feb, 2021
Reviews received
Received 12 Feb, 2021
Reviewers invited
Invitations sent on 12 Feb, 2021
Reviewer #1 agreed
On 12 Feb, 2021
Editor assigned
On 27 Jan, 2021
Submission checks complete
On 27 Jan, 2021
Editor invited
On 27 Jan, 2021
First submitted
On 26 Jan, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Orthopedic Surgery
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The network of intermediate filament proteins underlying the inner nuclear membrane forms the nuclear lamin. A and B-type lamins are the major components of the nuclear lamina. Lamins function in many nuclear activities. The role of lamin-A and transcription factors (NF-kB) as anti-apoptotic is well documented. Recently, lamin-A has also been considered as mechanosensor protein able to maintain nuclear integrity from mechanical insults.
We aimed to verify how lamin-A expression varies in healthy cuff cells and in those with different sized tears where various mechanical stresses are present.
Methods
Forty-three patients with rotator cuff tear (RCT) [23M-20F, mean age (SD): 63.5 (6.1)] were enrolled. Tissue samples excised from the most medial point of tear margins were analyzed for lamin-A expression by immunohistochemistry. Controls were represented by samples obtained by seven healthy supraspinatus tendons excised from patients submitted to reverse shoulder prosthesis implant [3M-4F, mean age (SD): 67.1 (6.3)]. The intensity of staining was graded, and an H-score was assigned. Statistical analysis was performed.
Results
Our study revealed a moderate intensity of lamin-A in the healthy cuff tendons, a higher expression of this protein in the small tears, and a significant decrease of lamin-A with increasing tear size (p<0.0001).
Conclusions
Our study emphasizes the importance of early repair of small RCTs since nuclear stability is maintained, and the cellular function is protected by lamin-A overexpression. High re-tear of massive cuff repair could be due to cellular apoptosis and nuclear modifications induced by lamin-A lack.
Level of Evidence: III
Figure 1
Figure 2
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