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Multiple functions of the scaffold protein Discs large 5 in the control of growth, cell polarity and cell adhesion in Drosophila melanogaster
Vincent MIrouse
CNRS
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5823-342X
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Background : Scaffold proteins support a variety of key processes during animal development. Mutant mouse for the MAGUK protein Discs large 5 (Dlg5) presents a general growth impairment and moderate morphogenetic defects.
Results : Here, we generated null mutants for Drosophila Dlg5 and show that it owns similar functions in growth and epithelial architecture. Dlg5 is required for growth at a cell autonomous level in several tissues and at the organism level, affecting cell size and proliferation. Our results are consistent with Dlg5 modulating hippo pathway in the wing disc, including the impact on cell size, a defect that is reproduced by the loss of yorkie. However, other observations indicate that Dlg5 regulates growth by at least another way that may involve Myc protein but nor PI3K neither TOR pathways. Moreover, epithelia cells mutant for Dlg5 also show a reduction of apical domain determinants, though not sufficient to induce a complete loss of cell polarity. Dlg5 is also essential, in the same cells, for the presence at Adherens junctions of N-Cadherin, but not E-Cadherin. Genetic analyses indicate that junction and polarity defects are independent.
Conclusions : Together our data show that Dlg5 own several conserved functions that are independent of each other in regulating growth, cell polarity and cell adhesion. Moreover, they reveal a differential regulation of E-cadherin and N-cadherin apical localization.
Keywords
Drosophila, MAGUK, yorkie, hippo, myc, polarity, adhesion, growth
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The accurate development of an organ or an organism requires a robust coordination size and shape control, both at the cell and tissue scales. Among the protein classes involved in such processes, many of them are scaffold proteins. These proteins are devoid of catalytic activity but contain multiple domains of protein-protein interaction [1]. They allow the formation of complexes that are determinant, for instance, for cell polarity, cell adhesion or that are used as a platform for various signaling events.
Membrane-associated guanylate kinase (MAGUK) proteins are typical examples of scaffold proteins [2]. MAGUK domain is a structural unit formed by SH3 domain next to a non-catalytically active guanylate kinase domain. These domains are usually flanked by one or several PDZ domains and potentially other protein-protein interaction domains. Some MAGUK domains recognize phosphopeptides, whereas some others work in cooperation with the adjacent PDZ domain, reinforcing the affinity of the latter for a specific partner [3, 4]. MAGUK proteins also emerge as important modulators of phase separation in cells [5, 6].
Discs Large (Dlg) is a MAGUK protein that was identified in Drosophila for its function in epithelial polarity as a determinant of the lateral domain and the neoplastic effect of its mutation [7-9]. Four paralogs of fly Dlg, Dlg1 to Dlg4, are found in mammals. A more divergent member of the family, Dlg5, is also found in fly and mammals with a conserved architecture: a coiled-coil domain, 4 PDZ domains and a MAGUK domain. Dlg5 studies in mammals emphasized a function in epithelial morphogenesis, the knock-out mouse showing mild defects of adherens junction and epithelial polarity in the kidney, the lung and the brain [10, 11]. Dlg5 is also required for N-Cadherin (N-Cad) delivery to the membrane during synaptogenesis [12]. A report in Drosophila using partial loss of function conditions in follicle cells also described moderate defect in the recruitment of apical determinants and junctional proteins [13]. This report suggested that Dlg5 acts mainly by a regulation of the apical determinant crumbs (crb). However, it is unclear whether the effect on polarity determinants and adherens junction are causally linked our whether they reflect independent functions of Dlg5 protein. Drosophila Dlg5 is also required for the proper collective cell migration of the border cells [14, 15]. Beside these morphogenetic defects, new born Dlg5 mice are considerably smaller than their wild-type littermates, suggesting an involvement in growth control [10]. Interestingly, Dlg5 has been functionally linked to the hippo pathway both in mammals and in flies, where it interacts and regulates negatively the MAST/hippo kinase [16]. However, whether such a hippo regulation could account for all the growth defects associated with the loss of Dlg5 is not known. Morever, Dlg5 was also identified as a positive regulator of the Target of Rapamycin complex 1 (TORC1) pathway in an in vitro RNAi screen [17].
Here, we identified Drosophila Dlg5 in an RNAi screen for genes linked to follicular epithelium development and we generated null mutants. These mutants allowed us to show that this gene is involved in the control of growth, both at the cellular and systemic levels. Our results suggest that Dlg5 regulates growth by at least two independent mechanisms. We also confirmed a moderate epithelial polarity defect and show a very strong and specific effect on N-Cad expression whereas E-Cadherin (E-Cad) is not affected. Importantly, we show that polarity defects and Adherens junction defects reflect independent functions of Dlg5.
The loss of Dlg5 alters cell autonomously follicle cell growth
We performed a reverse genetics screen to identify new genes involved in Drosophila follicular epithelium development, a tissue used as a generic model for various aspects of epithelium biology [18, 19]. Follicle cells form a monolayer epithelium surrounding germline cyst with the apical domain facing the germline. Follicle undergoes a rapid growth through 14 developmental stages, with a 1000-fold volume increase. Follicle cell growth is associated with proliferation until stage 6, then follicle cells become endoreplicative and larger. During the screen, we noticed that clones expressing RNAi against Dlg5 were small and the cells appeared also smaller than wild-type cells, especially after stage 6 (Fig 1A). This defect was quantified at stages 9-10A, showing an average reduction of 33% of the cell surface (Fig 1B). A similar defect was observed with a different RNAi line (Fig 1C). A P-element insertion in the 5’UTR of Dlg5 was available. This insertion was lethal and homozygous mitotic clones for this mutant also give small follicular cells (Fig 1D). However, the defect obtained with this mutant appeared more variable than with the RNAi lines, suggesting that it may correspond to a hypomorphic mutant. We generated P-element excisions and most of them restored the viability of the stock indicating that its lethality was associated with this insertion in Dlg5 gene. We also obtained several lethal imprecise excisions, Dlg5Ex5,Ex8,Ex13,Ex14 all, except Ex8, deleting the start codon and part of the coding sequence (Fig 1H). However, they also deleted part of the neighboring annotated gene (CG4970). This gene is only expressed in testis and is very poorly conserved, with no known domains and no ortholog in other insect species. Trans-heterozygous between a Minos element inserted in the coding sequence of this gene (MimicMI02472) and the deletions that we generated complement perfectly in terms of viability and fertility, indicating no essential function of CG4970. We also obtained rescue of Dlg5ex13 mutation lethality using a transgene with Dlg5 coding sequence under ubiquitin promoter and fused to GFP added in N-terminal (Ubi:GFP-Dlg5). Thus, we assumed that Dlg5ex13 could be considered as a bona fide null mutant. Mitotic clones for this allele contain cells with a reduced size (Fig 1E,G), a defect also rescued by Ubi:GFP-Dlg5 (Fig 1F,G), confirming the cell autonomous role of Dlg5 in follicle cell growth.
Dlg5 has a general function in growth
We next wondered whether Dlg5 function in cell growth could apply to other tissues. We induced Dlg5 knock-down specifically in the wing disc pouch, which corresponds to the future cells of the fly wing, using Nubbin:Gal4 [20]. It led to a dramatic reduction of the wing size (Fig 2A-B, D). We also induced mitotic clones for Dlg5Ex13 in the wing disc and quantified several parameters. Of notice, the mutant cells tend to form a row of cells rather to extend the clone in all directions, a defect reminiscent of what has been recently described for other mutants generating small cells [21] (Fig 2C). Mutant clones were systematically smaller than their twin and contained fewer cells (Fig2 E,G). Moreover, the mutant cells had, in average, a size reduced by 40% (Fig 2F). DCP-1 staining did not reveal apoptotic cells in Dlg5 mutant clones suggesting that the lower cell number per clone correspond to a growth and proliferation decrease (Fig 2H).
Finally, we looked at a transheterozygous combination of Dlg5 null alleles. First instar larvae hatch, are able to crawl around and are still alive 48 hours after egg deposition. However, their growth is strongly impaired, indicating a systemic requirement for Dlg5 (Fig 2J-K). Thus, altogether these results show that Dlg5 owns a general growth function, as its mammal counterpart, and that this function is performed in a cell-autonomous manner.
We aimed to define how Dgl5 modulates growth. It has been reported that Dlg5 modulated hippo pathway in the wing. However, this pathway is usually described as controlling of cell proliferation rather than cell growth. The Hippo signaling pathway regulates cell proliferation by inactivating Yorkie (Yki), the Drosophila Homolog of YAP. We therefore induced yki RNAi, and, as expected, its loss of function markedly reduces wing size and the estimated number of cells in the whole wing (Fig 3A-D). Importantly, we also noticed a reduction of cell size in the same range than what has been observed with the loss of Dlg5 (Fig 3E). Thus the hippo pathway also modulates cell size and could therefore explain Dlg5 contribution in this tissue. Yki is known to be required for normal follicle cell proliferation [22]. We induced null mutant clones and checked for cell size defects at stages 9-10. Comparing the cell surface indicates a moderate but significant effect of yki (Fig 3F-G). Together, these data clearly establish a role for yki in the control of cell size. However, this defect appears not stronger than the one induced by the loss of Dlg5 by RNAi (Fig 1B). Moreover, inhibition of hippo pathway has not been described as affecting systemic growth, leading to the hypothesis that Dlg5 may modulate growth by at least another means. Looking for other potential growth actors affected by the loss of Dlg5, we noticed that this gene was picked-up in a RNAi screen for TORC1 pathway regulators in S2 cells as affecting the level of S6K protein [17]. However, S6K level was unchanged in follicular cells or wing disc mutant cells for Dlg5ex13 (Fig 3H,L). Moreover, phosphorylation level of S6 were not affected in Dlg5 mutant follicle cells, indicating that Dlg5 does not modulate S6K activity and more generally the Tor pathway in this tissue (Fig 3I,L). We also, checked Insulin/PI3K pathway activity, which when affected, give similar defect both at the cellular and the systemic levels, but we did not observe any alteration of Phospho-Akt in Dlg5 mutant cells (Fig 3J,L). Thus, how Dlg5 influences growth in these cells remains to determine. Nonetheless, we noticed a reduction of Myc expression in Dlg5 mutant follicle cells, suggesting that it is required for the efficient signaling of one of the multiple pathways controlling Myc levels (Fig 3K,L) [23]. Importantly, Myc levels were never affected in mutant follicle cells for yki, demonstrating that Dlg5 effect is independent of Hippo pathway (Fig 3M). However, similar reduction was not detected in wing disc Dlg5 mutant cells (Fig 3N), indicating that Myc regulation cannot account for Dlg5 effect on growth in all tissues.
Dlg5 is required for the localization of apical polarity determinants
The fact that Dlg5 regulates growth both in mammals and in fly prompted us to check for an epithelial polarity phenotype, since such a defect has also been observed in Dlg5 mutant mouse. These defects have been detected for instance in the kidney or the lung, where a partial mislocalization of the apical determinant aPKC, a key component of the apical PAR complex, has been observed [10, 11]. Moreover, knock-down of Dlg5 in follicle cells also give similar phenotypes [13]. In follicular cells mutant for null mutant Dlg5Ex13 we saw a semi-penetrant reduction of the apical level of aPKC, the apical domain of these cells being inwards, at the contact with the germline (12/19 clones) (Fig 4A). The level of the apical determinant Crumbs (Crb) is also reduced (fig 4B). However, this apical reduction of apical determinants was never associated with an extension of lateral markers, such as Coracle (Cora), to the apical domain or to a mispositioning of the adherens junctions. Nonetheless, we pointed out that Cora was often upregulated in the mutant cells (21/33 clones) (Fig 1C,D), a defect never observed with another septate junction marker such as Dlg (n=9) (Fig 4D). Moreover, we never spotted multi-layers or round mutant cells. Also, mutant cells tend to flatten, a defect more often observed in young follicles (Fig 4C). Thus, although it is not sufficient to induce a complete loss of cell polarity in this tissue, Dlg5 null mutation can affect apical polarity determinants and cell morphology.
To characterize endogenous Dlg5 localization we generated an antibody against the third and fourth PDZ domains. In follicle cells, Dlg5 antibody reveals a dotty pattern, similarly to what has been described in mammals (Fig 5) [10]. This signal is specific because the antibody gives no signal in Dlg5 mutant follicle cells (Fig 6G). These dots were observed both inside the cell and at the cell cortex. Moreover, Dlg5 pattern was dynamic depending on the stages. During early stages (2-8) Dlg5 localizes at apical and lateral membranes at stage 1, and appears therefore in apical sooner than Crb (Fig 5A). Then the apical localization progressively decreases, and is barely detectable at stage 9 (Fig 2B). Consequently, at later stages this cortical localization is restricted to the lateral domain. Because Dlg5 is present apically as key apical determinants such as aPKC and Crb and affect their localization, we compared their localization in the apical plain of the follicle cells with higher resolution using Airyscan. We observed that aPKC and Crb are usually colocalized, especially at the marginal zone, an area of cell–cell contact apical to the adherens junctions (Fig 5C,F). This observation comes as confirmation that these proteins cooperate to define the apical domain [24]. In contrast, no evident colocalization is observed between Dlg5 and those two proteins and their localization even tend to be exclusive in the marginal zone, indicating that Dlg5 is not stably associated with these apical determinants (Fig 5D,E,G,H).
Dlg5 is required for N-cadherin localization independently of its effect on cell polarity
Looking at the Adherens junction, we found that E-Cad level was not affected (n=18) (Fig 6A). Follicle cells also expressed N-Cad, which is integrated in adherens junction, and Dlg5 has been functionally and molecularly linked to this cadherin in mammals [10, 12, 25]. We therefore looked at N-Cad and spotted an extremely strong and fully penetrant reduction in Dlg5 mutant cells (n>20) (Fig 6B). This effect correlates with the strength of Dlg5 loss of function because the reduction is weaker in hypomorphic conditions (Fig 6C). However, Dlg5 overexpression does not increase N-Cad levels at cell contacts and has no visible impact on cell size (Fig 6D). Thus, Dlg5 loss of function has a dramatic effect on N-Cad, but not on E-Cad, in the same cell type and at the same developmental stages. It indicates therefore a very specific effect on N-Cad membrane delivery or stability.
We therefore looked at a potential colocalization between N-Cad and Dlg5. Both are present mainly at the cell periphery. However, at the adherens junction plain of the cells Dlg5 is mainly found as medioapical dots whereas N-Cad surrounds the cells (Fig 6F). Just above, less N-cad is observed whereas Dlg5 becomes more enriched at the cortex. As a result, only a weak colocalization between the two proteins is observed with N-Cad being globally more apical than Dlg5 (Fig 6F). Thus, although Dlg5 has a very strong and specific impact on N-Cad, their potential association is likely transitory. We also noticed that when we induced Dlg5 mutant cell clones, Dlg5 disappears from the cell cortex even at the boundary with wild-type cells (Fig 6G). Classical interpretation for such observation is that Dlg5 is associated with proteins performing homophilic interactions between cells and that it is required for their localization, explaining its absence on the wild-type cell side. However, Dlg5 localization is not affected in N-Cad mutant clones (Fig 6H). Thus, Dlg5 is probably associated with another protein performing homophilic interactions and that is mainly localized at the lateral domain of the follicle cells.
The loss of Dlg5 affects both apical and adherens junction proteins. Since there is important cross-talks between the protein complexes acting at these two sites, we wondered whether those two defects were linked [26-28]. First, the loss of N-Cad had no effect on aPKC apical level, in agreement with the previous proposition that N-Cad and E-Cad are redundant in the follicle cells to maintain adherens junction and epithelial polarity (Fig 6I) [25]. Thus, Dlg5 impact on N-Cad does not explain the loss of apical proteins. Second, the reduction of apical determinant Crb and aPKC is much less expressive and penetrant than the loss of N-Cad and it is therefore unlikely the cause of such a defect. Patj mutation, a component of the Crb complex, leads to a very similar mild effect to Dlg5 mutation on aPKC and Crb apical levels and on cell morphology [29]. However, patj mutant cells show a normal level of N-Cad (Fig 6J). Thus, the reduction of apical determinants and of N-Cad observed in Dlg5 mutant cells correspond to two independent functions of the protein.
Altogether, our data show that Dlg5 owns several independent functions in Drosophila, suggesting that its scaffold abilities are used in different contexts. Of notice, these functions are somehow opposite to Dlg ones, both on growth and cell polarity, confirming that, despite the same naming, these two MAGUK proteins are unrelated.
First, Dlg5 requirement for growth is really strong, as revealed by the defect of the homozygous mutant larvae and its loss in the wing. It has been recently proposed that Dlg5 is involved in the hippo pathway, via a physical interaction and an inhibition of Hippo kinase by a mechanism that remains to be elucidated [16]. In fly, the main argument for such a regulation is a reduction of the Hippo reporter Expanded-lacZ in a Dlg5 loss of function in the wing disc. Consistently with this observation, yki loss of function induces a reduction of cell size both in the wing and in follicle cells. To our knowledge, this is the first report of a role of this gene in cell size control, which is actually accountable for about 15% of yki impact on tissue size. Interestingly, Hippo has been shown to be modulated by TORC1 or InR/PI3K pathways in different contexts [22, 30, 31]. Our results suggest that hippo modulation by these pathways may participate to explain their impact on cell size. Alternatively, these results could suggest that hippo pathway could conversely modulate these pathways. However, several lines of evidence suggest that the regulation of hippo is not sufficient to explain Dlg5 impact on growth. First, in the wing disc, we did not see an effect of Dlg5 null mutation on Myc, a well-established target of the Hippo pathway in this tissue [32]. Moreover, to the difference of yki mutant cells, we did not find evidence that Dlg5 mutant ones are eliminated by cell competition from the clones [33, 34]. Second, in the follicle cells, the situation looks at the opposite because Myc is modulated by Dlg5 but not by yki. Moreover, the effect of null mutation for yki on follicle cell size appears weaker than the one of Dlg5 hypomorphic conditions induced by RNAi. Finally, we observed a strong requirement of Dlg5 for larval growth, whereas such an effect of yki loss of function has never been reported.
Dlg5 mutant defects are reminiscent of strong mutants for cell growth such as the ones of key components of the TORC1 pathway, with a decrease of cell size and cell proliferation but no induction of apoptosis, even in clonal analysis that can reveal cell competition. However, we did not confirm in vivo the proposed link between Dlg5 and S6K stability or TORC1 activity [17]. Moreover, Myc expression is independent of TORC1 in follicle cells (Vachias, unpublished) whereas it is influenced by this complex in the wing disc [35], a contrary effect to the one of Dlg5. Thus, Dlg5 impact on growth is probably independent of TORC1. Similarly, misregulation of InR/PI3K pathway, which impacts cell size and proliferation, has not been observed in Dlg5 mutant cells. Thus, by which alternative pathway Dlg5 acts on cell growth and tissue size remains to be elucidated.
Our data using null mutants confirm the moderate impact of Dlg5 on apical-basal polarity observed in fly and mouse [10, 11, 13]. In follicle cells, these defects are really similar to the ones that we previously observed with Patj mutation, with both a semi-penetrant loss of apical determinants and a same cell shape defect in follicle cells [29]. This correspondence is in agreement with the proposition that Dlg5 regulates Crb complex [13]. Whereas Crb is essential to maintain follicle cell polarity, this complex is dispensable for the apical basal polarity of the wing cells, as it seems to be the case for Dlg5 because we observed no morphologic defect in this tissue [25, 36]. Interestingly, mouse Crb3 knock-out leads to cell polarity alteration in the kidney and the lung, which are also the epithelia known to be affected in Dlg5 mutant mouse, suggesting that the relationship between Dlg5 and Crb complex is conserved throughout evolution [37]. However, high resolution imaging of follicle cell apical domain reveals no colocalization between Dlg5 and Crb, suggesting that the effect of Dlg5 on Crb is indirect or relies on a very transient interaction. Moreover, apical localization of Dlg5 appears sooner than the one of Crb in young follicles suggesting different dynamics for the two proteins. Nonetheless, Crb is also a modulator of the Hippo pathway and it might be interesting in the future to explore the relationship between Dlg5, Crb and Hippo [38-42].
We also observed an extremely strong effect of Dlg5 null mutants on N-Cad localization at the membrane. Dlg5 does not affect E-Cad localization in the same cells and at the same stage, indicating a very specific effect. Although the specificity of the effect on N-Cad versus E-Cad was not established, available data in mammals also denote an impact of Dlg5 on N-Cad delivery associated with a physical interaction between the two proteins [10, 12]. Thus, the specific effect of Dlg5 on N-Cad is likely a conserved feature. E-Cad is usually associated with the acquisition of a stable epithelium architecture whereas N-Cad is more linked to collective cell migration and epithelium-mesenchyme transition [43, 44]. However, despite thousands of articles depicting E-cad versus N-cad expression, the molecular differences underlying these peculiarities are still an open question, relevant for developmental cell biology and cancer. The specific relationship between Dlg5 and N-Cad might provide a nice entry point to understand these differences.
Together our data show that Dlg5 own several conserved functions that are independent of each other in regulating growth, cell polarity and cell adhesion. Its effect on growth is likely pleiotropic, potentially acting on Hippo pathway but not only. Moreover, we revealed an effect of yki on cell size. Finally, this work reveals a differential regulation of E-cadherin and N-cadherin localization.
Molecular biology and antibody production
For transgenesis, Dlg5 coding sequence was amplified by PCR and cloned in phase in pUBi:GFP-Nterm-Gateway-AttB vector [45]. Transgenes were generated at AttP3-B landing site. Antibody were raised in rabbit against a fragment of Dlg5 corresponding to amino acids 1260 to 1600 fused to GST (Eurogentec).
Genetics
Fly were raised on wheat flour (8%) yeast extract (8%), Agar (1.1%) with antifungal and antibiotics. Dlg5 mutants were generated by imprecise excision of P{SUP-orP}KG00748. Excisions that were lethal when crossed with a deficiency covering Dlg5 locus were analyzed at the molecular level. Dlg5Ex13 contains a 1.5kb deletion going from upstream the transcription start to 200bp downstream of the translational start. The detailed genotypes, temperature and heat-shock conditions are given in supplemental table S1.
Immunostaining and imaging
Dissection and immunostaining were performed as described previously [46], adding CaCl2 1mM during fixation, excepted for Crb staining, which requires a specific fixation [25]. Primary antibodies used are DE-Cad (1/100, DHSB #DCAD2) and N-Cad (1/100 , DHSB, #DN-Ex), Cora (1/200, DHSB, #C615.16), cleaved DCP-1 (1/1000, Cell Signaling, #9578), S6K (1/2000, [47]), pS6K ( 1/400, [48] , pAKT (1/500, Cell signaling, #4054), , dMyc (1/500, SantaCruz BioTechnology #) , Crb (1/50, DHSB, #Cq4), aPKC (1/500, Santa Cruz Biotechnology, #C-20G) , Dlg (1/200, DHSB, #4F3), Dlg5 (1/ 500, this study). Images were taken using a Leica SP5 confocal microscope or a Zeiss 800 Airyscan. Wing images were acquired on a Zeiss Axio Scan Z1.
Cell segmentation and size quantification were performed on Fiji. For wing disc clones, total size of the clone and cell number were determined and cell size was inferred from these values. Comparison were performed with twin WT cells. For adult wings, Fijiwings was used to determine wing size and cell density in the same posterior region on all images [49]. Cell size and total cell numbers were inferred from these values. In follicles, all the cells were segmented based on Cora staining using Tissue Analyzer [50] and comparison was realized with WT or heterozygous cells in the vicinity of the mutant (or RNAi) cells. Fluorescent signal was measures in the whole cell surface excepted for pAKT for which only signal at junctions between mutant cells was compare to the signal between WT cells.
All the statistical analyses were performed on Prism using t-test. Figures were assembled with ScientiFig [51].
Dlg : Discs lage, Dlg5 : Discs large 5, Tor : Target or Rapamycin, E-Cad : Epithelial-Cadherin, N-Cad : Neuronal-Cadherin, MAGUK : Membrane Associated Guanylate Kinase, PDZ : Post-synaptic density protein 95, Discs large and Zona occludens 1, aPKC : atypical protein kinase C, crb : crumbs, GFP : Green Fluorescent Protein, DCP-1: Drosophila Caspase 1, S6K : S6 kinase, yki : yorkie ; Patj : Pals1-Associated Tight Junction, InR : Insulin Receptor, PI3K : Phosphatidylinositol 3-kinase
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Consent for Publication : NA
Availability of data and materials :
New materials (mutants, antibodies) will be shared upon request. The datasets used and/or analysed during the current study available from the corresponding author on reasonable request.
Competing interest
The authors declare that they have no competing interests
Fundings
This work was funded by the Association pour la Recherche contre le Cancer (ARC) and the Auvergne-Rhône-Alpes Region. This research was also financed by the French government IDEX-ISITE initiative 16-IDEX-0001 (CAP 20-25). The funding bodies played no role in the design of the study or the collection, analysis, and interpretation of data, or in writing the manuscript.
Authors contribution
PV and VM designed the project. PV, JLC, HV, GR, CV and VM performed the experiments. PV, JLC, HV, CV and VM interpreted the data. CV prepared the figures and VM wrote the article. All authors have read and approved the manuscript.
Acknowledgments
We thank A Teleman, I Palacios, L Luo and Y Hong for stocks and reagents. We also thank the confocal imaging facility of Clermont-Ferrand (CLIC) and Pierre Pouchin for his help with statistical analyses.
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Multiple functions of the scaffold protein Discs large 5 in the control of growth, cell polarity and cell adhesion in Drosophila melanogaster
Vincent MIrouse
CNRS
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ORCiD: https://orcid.org/0000-0001-5823-342X
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Background : Scaffold proteins support a variety of key processes during animal development. Mutant mouse for the MAGUK protein Discs large 5 (Dlg5) presents a general growth impairment and moderate morphogenetic defects.
Results : Here, we generated null mutants for Drosophila Dlg5 and show that it owns similar functions in growth and epithelial architecture. Dlg5 is required for growth at a cell autonomous level in several tissues and at the organism level, affecting cell size and proliferation. Our results are consistent with Dlg5 modulating hippo pathway in the wing disc, including the impact on cell size, a defect that is reproduced by the loss of yorkie. However, other observations indicate that Dlg5 regulates growth by at least another way that may involve Myc protein but nor PI3K neither TOR pathways. Moreover, epithelia cells mutant for Dlg5 also show a reduction of apical domain determinants, though not sufficient to induce a complete loss of cell polarity. Dlg5 is also essential, in the same cells, for the presence at Adherens junctions of N-Cadherin, but not E-Cadherin. Genetic analyses indicate that junction and polarity defects are independent.
Conclusions : Together our data show that Dlg5 own several conserved functions that are independent of each other in regulating growth, cell polarity and cell adhesion. Moreover, they reveal a differential regulation of E-cadherin and N-cadherin apical localization.
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The accurate development of an organ or an organism requires a robust coordination size and shape control, both at the cell and tissue scales. Among the protein classes involved in such processes, many of them are scaffold proteins. These proteins are devoid of catalytic activity but contain multiple domains of protein-protein interaction [1]. They allow the formation of complexes that are determinant, for instance, for cell polarity, cell adhesion or that are used as a platform for various signaling events.
Membrane-associated guanylate kinase (MAGUK) proteins are typical examples of scaffold proteins [2]. MAGUK domain is a structural unit formed by SH3 domain next to a non-catalytically active guanylate kinase domain. These domains are usually flanked by one or several PDZ domains and potentially other protein-protein interaction domains. Some MAGUK domains recognize phosphopeptides, whereas some others work in cooperation with the adjacent PDZ domain, reinforcing the affinity of the latter for a specific partner [3, 4]. MAGUK proteins also emerge as important modulators of phase separation in cells [5, 6].
Discs Large (Dlg) is a MAGUK protein that was identified in Drosophila for its function in epithelial polarity as a determinant of the lateral domain and the neoplastic effect of its mutation [7-9]. Four paralogs of fly Dlg, Dlg1 to Dlg4, are found in mammals. A more divergent member of the family, Dlg5, is also found in fly and mammals with a conserved architecture: a coiled-coil domain, 4 PDZ domains and a MAGUK domain. Dlg5 studies in mammals emphasized a function in epithelial morphogenesis, the knock-out mouse showing mild defects of adherens junction and epithelial polarity in the kidney, the lung and the brain [10, 11]. Dlg5 is also required for N-Cadherin (N-Cad) delivery to the membrane during synaptogenesis [12]. A report in Drosophila using partial loss of function conditions in follicle cells also described moderate defect in the recruitment of apical determinants and junctional proteins [13]. This report suggested that Dlg5 acts mainly by a regulation of the apical determinant crumbs (crb). However, it is unclear whether the effect on polarity determinants and adherens junction are causally linked our whether they reflect independent functions of Dlg5 protein. Drosophila Dlg5 is also required for the proper collective cell migration of the border cells [14, 15]. Beside these morphogenetic defects, new born Dlg5 mice are considerably smaller than their wild-type littermates, suggesting an involvement in growth control [10]. Interestingly, Dlg5 has been functionally linked to the hippo pathway both in mammals and in flies, where it interacts and regulates negatively the MAST/hippo kinase [16]. However, whether such a hippo regulation could account for all the growth defects associated with the loss of Dlg5 is not known. Morever, Dlg5 was also identified as a positive regulator of the Target of Rapamycin complex 1 (TORC1) pathway in an in vitro RNAi screen [17].
Here, we identified Drosophila Dlg5 in an RNAi screen for genes linked to follicular epithelium development and we generated null mutants. These mutants allowed us to show that this gene is involved in the control of growth, both at the cellular and systemic levels. Our results suggest that Dlg5 regulates growth by at least two independent mechanisms. We also confirmed a moderate epithelial polarity defect and show a very strong and specific effect on N-Cad expression whereas E-Cadherin (E-Cad) is not affected. Importantly, we show that polarity defects and Adherens junction defects reflect independent functions of Dlg5.
The loss of Dlg5 alters cell autonomously follicle cell growth
We performed a reverse genetics screen to identify new genes involved in Drosophila follicular epithelium development, a tissue used as a generic model for various aspects of epithelium biology [18, 19]. Follicle cells form a monolayer epithelium surrounding germline cyst with the apical domain facing the germline. Follicle undergoes a rapid growth through 14 developmental stages, with a 1000-fold volume increase. Follicle cell growth is associated with proliferation until stage 6, then follicle cells become endoreplicative and larger. During the screen, we noticed that clones expressing RNAi against Dlg5 were small and the cells appeared also smaller than wild-type cells, especially after stage 6 (Fig 1A). This defect was quantified at stages 9-10A, showing an average reduction of 33% of the cell surface (Fig 1B). A similar defect was observed with a different RNAi line (Fig 1C). A P-element insertion in the 5’UTR of Dlg5 was available. This insertion was lethal and homozygous mitotic clones for this mutant also give small follicular cells (Fig 1D). However, the defect obtained with this mutant appeared more variable than with the RNAi lines, suggesting that it may correspond to a hypomorphic mutant. We generated P-element excisions and most of them restored the viability of the stock indicating that its lethality was associated with this insertion in Dlg5 gene. We also obtained several lethal imprecise excisions, Dlg5Ex5,Ex8,Ex13,Ex14 all, except Ex8, deleting the start codon and part of the coding sequence (Fig 1H). However, they also deleted part of the neighboring annotated gene (CG4970). This gene is only expressed in testis and is very poorly conserved, with no known domains and no ortholog in other insect species. Trans-heterozygous between a Minos element inserted in the coding sequence of this gene (MimicMI02472) and the deletions that we generated complement perfectly in terms of viability and fertility, indicating no essential function of CG4970. We also obtained rescue of Dlg5ex13 mutation lethality using a transgene with Dlg5 coding sequence under ubiquitin promoter and fused to GFP added in N-terminal (Ubi:GFP-Dlg5). Thus, we assumed that Dlg5ex13 could be considered as a bona fide null mutant. Mitotic clones for this allele contain cells with a reduced size (Fig 1E,G), a defect also rescued by Ubi:GFP-Dlg5 (Fig 1F,G), confirming the cell autonomous role of Dlg5 in follicle cell growth.
Dlg5 has a general function in growth
We next wondered whether Dlg5 function in cell growth could apply to other tissues. We induced Dlg5 knock-down specifically in the wing disc pouch, which corresponds to the future cells of the fly wing, using Nubbin:Gal4 [20]. It led to a dramatic reduction of the wing size (Fig 2A-B, D). We also induced mitotic clones for Dlg5Ex13 in the wing disc and quantified several parameters. Of notice, the mutant cells tend to form a row of cells rather to extend the clone in all directions, a defect reminiscent of what has been recently described for other mutants generating small cells [21] (Fig 2C). Mutant clones were systematically smaller than their twin and contained fewer cells (Fig2 E,G). Moreover, the mutant cells had, in average, a size reduced by 40% (Fig 2F). DCP-1 staining did not reveal apoptotic cells in Dlg5 mutant clones suggesting that the lower cell number per clone correspond to a growth and proliferation decrease (Fig 2H).
Finally, we looked at a transheterozygous combination of Dlg5 null alleles. First instar larvae hatch, are able to crawl around and are still alive 48 hours after egg deposition. However, their growth is strongly impaired, indicating a systemic requirement for Dlg5 (Fig 2J-K). Thus, altogether these results show that Dlg5 owns a general growth function, as its mammal counterpart, and that this function is performed in a cell-autonomous manner.
We aimed to define how Dgl5 modulates growth. It has been reported that Dlg5 modulated hippo pathway in the wing. However, this pathway is usually described as controlling of cell proliferation rather than cell growth. The Hippo signaling pathway regulates cell proliferation by inactivating Yorkie (Yki), the Drosophila Homolog of YAP. We therefore induced yki RNAi, and, as expected, its loss of function markedly reduces wing size and the estimated number of cells in the whole wing (Fig 3A-D). Importantly, we also noticed a reduction of cell size in the same range than what has been observed with the loss of Dlg5 (Fig 3E). Thus the hippo pathway also modulates cell size and could therefore explain Dlg5 contribution in this tissue. Yki is known to be required for normal follicle cell proliferation [22]. We induced null mutant clones and checked for cell size defects at stages 9-10. Comparing the cell surface indicates a moderate but significant effect of yki (Fig 3F-G). Together, these data clearly establish a role for yki in the control of cell size. However, this defect appears not stronger than the one induced by the loss of Dlg5 by RNAi (Fig 1B). Moreover, inhibition of hippo pathway has not been described as affecting systemic growth, leading to the hypothesis that Dlg5 may modulate growth by at least another means. Looking for other potential growth actors affected by the loss of Dlg5, we noticed that this gene was picked-up in a RNAi screen for TORC1 pathway regulators in S2 cells as affecting the level of S6K protein [17]. However, S6K level was unchanged in follicular cells or wing disc mutant cells for Dlg5ex13 (Fig 3H,L). Moreover, phosphorylation level of S6 were not affected in Dlg5 mutant follicle cells, indicating that Dlg5 does not modulate S6K activity and more generally the Tor pathway in this tissue (Fig 3I,L). We also, checked Insulin/PI3K pathway activity, which when affected, give similar defect both at the cellular and the systemic levels, but we did not observe any alteration of Phospho-Akt in Dlg5 mutant cells (Fig 3J,L). Thus, how Dlg5 influences growth in these cells remains to determine. Nonetheless, we noticed a reduction of Myc expression in Dlg5 mutant follicle cells, suggesting that it is required for the efficient signaling of one of the multiple pathways controlling Myc levels (Fig 3K,L) [23]. Importantly, Myc levels were never affected in mutant follicle cells for yki, demonstrating that Dlg5 effect is independent of Hippo pathway (Fig 3M). However, similar reduction was not detected in wing disc Dlg5 mutant cells (Fig 3N), indicating that Myc regulation cannot account for Dlg5 effect on growth in all tissues.
Dlg5 is required for the localization of apical polarity determinants
The fact that Dlg5 regulates growth both in mammals and in fly prompted us to check for an epithelial polarity phenotype, since such a defect has also been observed in Dlg5 mutant mouse. These defects have been detected for instance in the kidney or the lung, where a partial mislocalization of the apical determinant aPKC, a key component of the apical PAR complex, has been observed [10, 11]. Moreover, knock-down of Dlg5 in follicle cells also give similar phenotypes [13]. In follicular cells mutant for null mutant Dlg5Ex13 we saw a semi-penetrant reduction of the apical level of aPKC, the apical domain of these cells being inwards, at the contact with the germline (12/19 clones) (Fig 4A). The level of the apical determinant Crumbs (Crb) is also reduced (fig 4B). However, this apical reduction of apical determinants was never associated with an extension of lateral markers, such as Coracle (Cora), to the apical domain or to a mispositioning of the adherens junctions. Nonetheless, we pointed out that Cora was often upregulated in the mutant cells (21/33 clones) (Fig 1C,D), a defect never observed with another septate junction marker such as Dlg (n=9) (Fig 4D). Moreover, we never spotted multi-layers or round mutant cells. Also, mutant cells tend to flatten, a defect more often observed in young follicles (Fig 4C). Thus, although it is not sufficient to induce a complete loss of cell polarity in this tissue, Dlg5 null mutation can affect apical polarity determinants and cell morphology.
To characterize endogenous Dlg5 localization we generated an antibody against the third and fourth PDZ domains. In follicle cells, Dlg5 antibody reveals a dotty pattern, similarly to what has been described in mammals (Fig 5) [10]. This signal is specific because the antibody gives no signal in Dlg5 mutant follicle cells (Fig 6G). These dots were observed both inside the cell and at the cell cortex. Moreover, Dlg5 pattern was dynamic depending on the stages. During early stages (2-8) Dlg5 localizes at apical and lateral membranes at stage 1, and appears therefore in apical sooner than Crb (Fig 5A). Then the apical localization progressively decreases, and is barely detectable at stage 9 (Fig 2B). Consequently, at later stages this cortical localization is restricted to the lateral domain. Because Dlg5 is present apically as key apical determinants such as aPKC and Crb and affect their localization, we compared their localization in the apical plain of the follicle cells with higher resolution using Airyscan. We observed that aPKC and Crb are usually colocalized, especially at the marginal zone, an area of cell–cell contact apical to the adherens junctions (Fig 5C,F). This observation comes as confirmation that these proteins cooperate to define the apical domain [24]. In contrast, no evident colocalization is observed between Dlg5 and those two proteins and their localization even tend to be exclusive in the marginal zone, indicating that Dlg5 is not stably associated with these apical determinants (Fig 5D,E,G,H).
Dlg5 is required for N-cadherin localization independently of its effect on cell polarity
Looking at the Adherens junction, we found that E-Cad level was not affected (n=18) (Fig 6A). Follicle cells also expressed N-Cad, which is integrated in adherens junction, and Dlg5 has been functionally and molecularly linked to this cadherin in mammals [10, 12, 25]. We therefore looked at N-Cad and spotted an extremely strong and fully penetrant reduction in Dlg5 mutant cells (n>20) (Fig 6B). This effect correlates with the strength of Dlg5 loss of function because the reduction is weaker in hypomorphic conditions (Fig 6C). However, Dlg5 overexpression does not increase N-Cad levels at cell contacts and has no visible impact on cell size (Fig 6D). Thus, Dlg5 loss of function has a dramatic effect on N-Cad, but not on E-Cad, in the same cell type and at the same developmental stages. It indicates therefore a very specific effect on N-Cad membrane delivery or stability.
We therefore looked at a potential colocalization between N-Cad and Dlg5. Both are present mainly at the cell periphery. However, at the adherens junction plain of the cells Dlg5 is mainly found as medioapical dots whereas N-Cad surrounds the cells (Fig 6F). Just above, less N-cad is observed whereas Dlg5 becomes more enriched at the cortex. As a result, only a weak colocalization between the two proteins is observed with N-Cad being globally more apical than Dlg5 (Fig 6F). Thus, although Dlg5 has a very strong and specific impact on N-Cad, their potential association is likely transitory. We also noticed that when we induced Dlg5 mutant cell clones, Dlg5 disappears from the cell cortex even at the boundary with wild-type cells (Fig 6G). Classical interpretation for such observation is that Dlg5 is associated with proteins performing homophilic interactions between cells and that it is required for their localization, explaining its absence on the wild-type cell side. However, Dlg5 localization is not affected in N-Cad mutant clones (Fig 6H). Thus, Dlg5 is probably associated with another protein performing homophilic interactions and that is mainly localized at the lateral domain of the follicle cells.
The loss of Dlg5 affects both apical and adherens junction proteins. Since there is important cross-talks between the protein complexes acting at these two sites, we wondered whether those two defects were linked [26-28]. First, the loss of N-Cad had no effect on aPKC apical level, in agreement with the previous proposition that N-Cad and E-Cad are redundant in the follicle cells to maintain adherens junction and epithelial polarity (Fig 6I) [25]. Thus, Dlg5 impact on N-Cad does not explain the loss of apical proteins. Second, the reduction of apical determinant Crb and aPKC is much less expressive and penetrant than the loss of N-Cad and it is therefore unlikely the cause of such a defect. Patj mutation, a component of the Crb complex, leads to a very similar mild effect to Dlg5 mutation on aPKC and Crb apical levels and on cell morphology [29]. However, patj mutant cells show a normal level of N-Cad (Fig 6J). Thus, the reduction of apical determinants and of N-Cad observed in Dlg5 mutant cells correspond to two independent functions of the protein.
Altogether, our data show that Dlg5 owns several independent functions in Drosophila, suggesting that its scaffold abilities are used in different contexts. Of notice, these functions are somehow opposite to Dlg ones, both on growth and cell polarity, confirming that, despite the same naming, these two MAGUK proteins are unrelated.
First, Dlg5 requirement for growth is really strong, as revealed by the defect of the homozygous mutant larvae and its loss in the wing. It has been recently proposed that Dlg5 is involved in the hippo pathway, via a physical interaction and an inhibition of Hippo kinase by a mechanism that remains to be elucidated [16]. In fly, the main argument for such a regulation is a reduction of the Hippo reporter Expanded-lacZ in a Dlg5 loss of function in the wing disc. Consistently with this observation, yki loss of function induces a reduction of cell size both in the wing and in follicle cells. To our knowledge, this is the first report of a role of this gene in cell size control, which is actually accountable for about 15% of yki impact on tissue size. Interestingly, Hippo has been shown to be modulated by TORC1 or InR/PI3K pathways in different contexts [22, 30, 31]. Our results suggest that hippo modulation by these pathways may participate to explain their impact on cell size. Alternatively, these results could suggest that hippo pathway could conversely modulate these pathways. However, several lines of evidence suggest that the regulation of hippo is not sufficient to explain Dlg5 impact on growth. First, in the wing disc, we did not see an effect of Dlg5 null mutation on Myc, a well-established target of the Hippo pathway in this tissue [32]. Moreover, to the difference of yki mutant cells, we did not find evidence that Dlg5 mutant ones are eliminated by cell competition from the clones [33, 34]. Second, in the follicle cells, the situation looks at the opposite because Myc is modulated by Dlg5 but not by yki. Moreover, the effect of null mutation for yki on follicle cell size appears weaker than the one of Dlg5 hypomorphic conditions induced by RNAi. Finally, we observed a strong requirement of Dlg5 for larval growth, whereas such an effect of yki loss of function has never been reported.
Dlg5 mutant defects are reminiscent of strong mutants for cell growth such as the ones of key components of the TORC1 pathway, with a decrease of cell size and cell proliferation but no induction of apoptosis, even in clonal analysis that can reveal cell competition. However, we did not confirm in vivo the proposed link between Dlg5 and S6K stability or TORC1 activity [17]. Moreover, Myc expression is independent of TORC1 in follicle cells (Vachias, unpublished) whereas it is influenced by this complex in the wing disc [35], a contrary effect to the one of Dlg5. Thus, Dlg5 impact on growth is probably independent of TORC1. Similarly, misregulation of InR/PI3K pathway, which impacts cell size and proliferation, has not been observed in Dlg5 mutant cells. Thus, by which alternative pathway Dlg5 acts on cell growth and tissue size remains to be elucidated.
Our data using null mutants confirm the moderate impact of Dlg5 on apical-basal polarity observed in fly and mouse [10, 11, 13]. In follicle cells, these defects are really similar to the ones that we previously observed with Patj mutation, with both a semi-penetrant loss of apical determinants and a same cell shape defect in follicle cells [29]. This correspondence is in agreement with the proposition that Dlg5 regulates Crb complex [13]. Whereas Crb is essential to maintain follicle cell polarity, this complex is dispensable for the apical basal polarity of the wing cells, as it seems to be the case for Dlg5 because we observed no morphologic defect in this tissue [25, 36]. Interestingly, mouse Crb3 knock-out leads to cell polarity alteration in the kidney and the lung, which are also the epithelia known to be affected in Dlg5 mutant mouse, suggesting that the relationship between Dlg5 and Crb complex is conserved throughout evolution [37]. However, high resolution imaging of follicle cell apical domain reveals no colocalization between Dlg5 and Crb, suggesting that the effect of Dlg5 on Crb is indirect or relies on a very transient interaction. Moreover, apical localization of Dlg5 appears sooner than the one of Crb in young follicles suggesting different dynamics for the two proteins. Nonetheless, Crb is also a modulator of the Hippo pathway and it might be interesting in the future to explore the relationship between Dlg5, Crb and Hippo [38-42].
We also observed an extremely strong effect of Dlg5 null mutants on N-Cad localization at the membrane. Dlg5 does not affect E-Cad localization in the same cells and at the same stage, indicating a very specific effect. Although the specificity of the effect on N-Cad versus E-Cad was not established, available data in mammals also denote an impact of Dlg5 on N-Cad delivery associated with a physical interaction between the two proteins [10, 12]. Thus, the specific effect of Dlg5 on N-Cad is likely a conserved feature. E-Cad is usually associated with the acquisition of a stable epithelium architecture whereas N-Cad is more linked to collective cell migration and epithelium-mesenchyme transition [43, 44]. However, despite thousands of articles depicting E-cad versus N-cad expression, the molecular differences underlying these peculiarities are still an open question, relevant for developmental cell biology and cancer. The specific relationship between Dlg5 and N-Cad might provide a nice entry point to understand these differences.
Together our data show that Dlg5 own several conserved functions that are independent of each other in regulating growth, cell polarity and cell adhesion. Its effect on growth is likely pleiotropic, potentially acting on Hippo pathway but not only. Moreover, we revealed an effect of yki on cell size. Finally, this work reveals a differential regulation of E-cadherin and N-cadherin localization.
Molecular biology and antibody production
For transgenesis, Dlg5 coding sequence was amplified by PCR and cloned in phase in pUBi:GFP-Nterm-Gateway-AttB vector [45]. Transgenes were generated at AttP3-B landing site. Antibody were raised in rabbit against a fragment of Dlg5 corresponding to amino acids 1260 to 1600 fused to GST (Eurogentec).
Genetics
Fly were raised on wheat flour (8%) yeast extract (8%), Agar (1.1%) with antifungal and antibiotics. Dlg5 mutants were generated by imprecise excision of P{SUP-orP}KG00748. Excisions that were lethal when crossed with a deficiency covering Dlg5 locus were analyzed at the molecular level. Dlg5Ex13 contains a 1.5kb deletion going from upstream the transcription start to 200bp downstream of the translational start. The detailed genotypes, temperature and heat-shock conditions are given in supplemental table S1.
Immunostaining and imaging
Dissection and immunostaining were performed as described previously [46], adding CaCl2 1mM during fixation, excepted for Crb staining, which requires a specific fixation [25]. Primary antibodies used are DE-Cad (1/100, DHSB #DCAD2) and N-Cad (1/100 , DHSB, #DN-Ex), Cora (1/200, DHSB, #C615.16), cleaved DCP-1 (1/1000, Cell Signaling, #9578), S6K (1/2000, [47]), pS6K ( 1/400, [48] , pAKT (1/500, Cell signaling, #4054), , dMyc (1/500, SantaCruz BioTechnology #) , Crb (1/50, DHSB, #Cq4), aPKC (1/500, Santa Cruz Biotechnology, #C-20G) , Dlg (1/200, DHSB, #4F3), Dlg5 (1/ 500, this study). Images were taken using a Leica SP5 confocal microscope or a Zeiss 800 Airyscan. Wing images were acquired on a Zeiss Axio Scan Z1.
Cell segmentation and size quantification were performed on Fiji. For wing disc clones, total size of the clone and cell number were determined and cell size was inferred from these values. Comparison were performed with twin WT cells. For adult wings, Fijiwings was used to determine wing size and cell density in the same posterior region on all images [49]. Cell size and total cell numbers were inferred from these values. In follicles, all the cells were segmented based on Cora staining using Tissue Analyzer [50] and comparison was realized with WT or heterozygous cells in the vicinity of the mutant (or RNAi) cells. Fluorescent signal was measures in the whole cell surface excepted for pAKT for which only signal at junctions between mutant cells was compare to the signal between WT cells.
All the statistical analyses were performed on Prism using t-test. Figures were assembled with ScientiFig [51].
Dlg : Discs lage, Dlg5 : Discs large 5, Tor : Target or Rapamycin, E-Cad : Epithelial-Cadherin, N-Cad : Neuronal-Cadherin, MAGUK : Membrane Associated Guanylate Kinase, PDZ : Post-synaptic density protein 95, Discs large and Zona occludens 1, aPKC : atypical protein kinase C, crb : crumbs, GFP : Green Fluorescent Protein, DCP-1: Drosophila Caspase 1, S6K : S6 kinase, yki : yorkie ; Patj : Pals1-Associated Tight Junction, InR : Insulin Receptor, PI3K : Phosphatidylinositol 3-kinase
Ethics approval and consent to participate : NA
Consent for Publication : NA
Availability of data and materials :
New materials (mutants, antibodies) will be shared upon request. The datasets used and/or analysed during the current study available from the corresponding author on reasonable request.
Competing interest
The authors declare that they have no competing interests
Fundings
This work was funded by the Association pour la Recherche contre le Cancer (ARC) and the Auvergne-Rhône-Alpes Region. This research was also financed by the French government IDEX-ISITE initiative 16-IDEX-0001 (CAP 20-25). The funding bodies played no role in the design of the study or the collection, analysis, and interpretation of data, or in writing the manuscript.
Authors contribution
PV and VM designed the project. PV, JLC, HV, GR, CV and VM performed the experiments. PV, JLC, HV, CV and VM interpreted the data. CV prepared the figures and VM wrote the article. All authors have read and approved the manuscript.
Acknowledgments
We thank A Teleman, I Palacios, L Luo and Y Hong for stocks and reagents. We also thank the confocal imaging facility of Clermont-Ferrand (CLIC) and Pierre Pouchin for his help with statistical analyses.
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