This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Mohammad Ali Azarbayjani
Islamic Azad University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3502-7487
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: Deep-frying is a common cooking method accompanied by production of carcinogenesis substance such as acrolein. Acrolein is a toxic byproduct of lipid peroxidation that is involved in the development of pulmonary, cardiac, and neurodegenerative diseases. This study aimed to explore the effect of aerobic exercise (EXE) and octopamine (OCT) on caspase 3 and pro-caspase 3 expression levels in the heart tissue of rats were fed deep-frying oil (DFO).
Methods: 30 male Wistar rats were divided into 5 groups (n=6 in each) including 1) control (CO), 2) DFO, 3) DFO+EXE, 4) DFO+OCT and 5) DFO+EXE+OCT. The apoptotic effects of DFO on heart and cardiomyocytes’ fibers were examined by TUNEL assay and Masson's trichrome staining respectively. Also caspase 3 and pro-caspase 3 genes and proteins expression in all groups were evaluated using quantitative real-time PCR and western blotting method, respectively.
Results: Data showed a significant increase in apoptotic cells in the DFO group (P <0.05). Masson's trichrome stain analysis demonstrated that the number of cardiomyocytes' fibers are decreased, and collagen deposition is increased in the DFO group. In comparison, the collagen percentage was significantly reduced in DFO+EXE, DFO +OCT and DFO+EXE+OCT groups. Also, the expression level of caspase 3 and pro-caspase 3 was significantly decreased in DFO+EXE+OCT group (P <0.05).
Conclusions: The results of this study show that DFO lead to programmed cell death via the activation of caspases in heart tissue. However, it seems that aerobic exercise with octopamine supplementation improves heart tissue through decreasing in the expression of caspase 3 and pro-caspase 3 that inhibit apoptosis.
Keywords
Cardiomyocytes, Aerobic exercise, Apoptosis, Octopamine, Deep-frying oil
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Mohammad Ali Azarbayjani
Islamic Azad University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3502-7487
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 4
Posted 25 Sep, 2020
No community comments so far
No comments provided
No comments provided
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Deep-frying is a common cooking method accompanied by production of carcinogenesis substance such as acrolein. Acrolein is a toxic byproduct of lipid peroxidation that is involved in the development of pulmonary, cardiac, and neurodegenerative diseases. This study aimed to explore the effect of aerobic exercise (EXE) and octopamine (OCT) on caspase 3 and pro-caspase 3 expression levels in the heart tissue of rats were fed deep-frying oil (DFO).
Methods: 30 male Wistar rats were divided into 5 groups (n=6 in each) including 1) control (CO), 2) DFO, 3) DFO+EXE, 4) DFO+OCT and 5) DFO+EXE+OCT. The apoptotic effects of DFO on heart and cardiomyocytes’ fibers were examined by TUNEL assay and Masson's trichrome staining respectively. Also caspase 3 and pro-caspase 3 genes and proteins expression in all groups were evaluated using quantitative real-time PCR and western blotting method, respectively.
Results: Data showed a significant increase in apoptotic cells in the DFO group (P <0.05). Masson's trichrome stain analysis demonstrated that the number of cardiomyocytes' fibers are decreased, and collagen deposition is increased in the DFO group. In comparison, the collagen percentage was significantly reduced in DFO+EXE, DFO +OCT and DFO+EXE+OCT groups. Also, the expression level of caspase 3 and pro-caspase 3 was significantly decreased in DFO+EXE+OCT group (P <0.05).
Conclusions: The results of this study show that DFO lead to programmed cell death via the activation of caspases in heart tissue. However, it seems that aerobic exercise with octopamine supplementation improves heart tissue through decreasing in the expression of caspase 3 and pro-caspase 3 that inhibit apoptosis.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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