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Research
Mi-La Cho
Catholic University of Korea
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5715-3989
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: To evaluate the immunomodulatory effect of Lactobacillus sakei in a mouse model of rheumatoid arthritis (RA) and in human immune cells.
Methods: We evaluated whether L. sakei reduced the severity of collagen-induced arthritis (CIA) and modulated interleukin (IL)-17 and IL-10 levels, as well as whether it affected the differentiation of CD4 + T cells and regulatory B cells. We evaluated osteoclastogenesis after culturing bone marrow-derived mononuclear cells with L. sakei.
Results: The differentiation of T helper 17 cells and the serum level of IL-17 were suppressed by L. sakei in both human peripheral blood mononuclear cells and mouse splenocytes. The serum level of IL-10 was significantly increased in the L. sakei -treated group, whereas the regulatory T cell population was unchanged. The population of regulatory B cells significantly increased the in L. sakei -treated group. Oral administration of L. sakei reduced the arthritis incidence and score in mice with CIA. Finally, osteoclastogenesis and the mRNA levels of osteoclast-related genes were suppressed in the L. sakei -treated group.
Conclusion: L. sakei exerted an anti-inflammatory effect in an animal model of RA, regulated Th17 and regulatory B cell differentiation, and suppressed osteoclastogenesis. Our findings suggest that L. sakei has therapeutic potential for RA.
Keywords
Rheumatoid arthritis, microbiome, Lactobacillus sakei, T helper 17 cell, regulatory B cell
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CURRENT STATUS: Published
View the published article at Journal of Translational Medicine.
Version 2
Posted 04 Jun, 2020
No community comments so far
Editorial decision: Minor revision
On 22 Jun, 2020
Review #1 received
Received 13 Jun, 2020
Review #2 received
Received 13 Jun, 2020
Reviewers invited
Invitations sent on 08 Jun, 2020
Reviewer #1 agreed
On 08 Jun, 2020
Reviewer #2 agreed
On 08 Jun, 2020
Editor assigned
On 29 May, 2020
Submission checks complete
On 28 May, 2020
Editor invited
On 28 May, 2020
No comments provided
Editorial decision: Major revision
On 22 Apr, 2020
Review #2 received
Received 18 Apr, 2020
Reviewer #2 agreed
On 05 Apr, 2020
Review #1 received
Received 05 Apr, 2020
Reviewers invited
Invitations sent on 25 Mar, 2020
Reviewer #1 agreed
On 25 Mar, 2020
Editor assigned
On 13 Mar, 2020
Editor invited
On 12 Mar, 2020
Submission checks complete
On 11 Mar, 2020
First submitted
On 09 Mar, 2020
Metrics
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Journal of Translational Medicine.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Mi-La Cho
Catholic University of Korea
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5715-3989
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Journal of Translational Medicine.
Version 2
Posted 04 Jun, 2020
No community comments so far
Editorial decision: Minor revision
On 22 Jun, 2020
Review #1 received
Received 13 Jun, 2020
Review #2 received
Received 13 Jun, 2020
Reviewers invited
Invitations sent on 08 Jun, 2020
Reviewer #1 agreed
On 08 Jun, 2020
Reviewer #2 agreed
On 08 Jun, 2020
Editor assigned
On 29 May, 2020
Submission checks complete
On 28 May, 2020
Editor invited
On 28 May, 2020
No comments provided
Editorial decision: Major revision
On 22 Apr, 2020
Review #2 received
Received 18 Apr, 2020
Reviewer #2 agreed
On 05 Apr, 2020
Review #1 received
Received 05 Apr, 2020
Reviewers invited
Invitations sent on 25 Mar, 2020
Reviewer #1 agreed
On 25 Mar, 2020
Editor assigned
On 13 Mar, 2020
Editor invited
On 12 Mar, 2020
Submission checks complete
On 11 Mar, 2020
First submitted
On 09 Mar, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Journal of Translational Medicine.
Background: To evaluate the immunomodulatory effect of Lactobacillus sakei in a mouse model of rheumatoid arthritis (RA) and in human immune cells.
Methods: We evaluated whether L. sakei reduced the severity of collagen-induced arthritis (CIA) and modulated interleukin (IL)-17 and IL-10 levels, as well as whether it affected the differentiation of CD4 + T cells and regulatory B cells. We evaluated osteoclastogenesis after culturing bone marrow-derived mononuclear cells with L. sakei.
Results: The differentiation of T helper 17 cells and the serum level of IL-17 were suppressed by L. sakei in both human peripheral blood mononuclear cells and mouse splenocytes. The serum level of IL-10 was significantly increased in the L. sakei -treated group, whereas the regulatory T cell population was unchanged. The population of regulatory B cells significantly increased the in L. sakei -treated group. Oral administration of L. sakei reduced the arthritis incidence and score in mice with CIA. Finally, osteoclastogenesis and the mRNA levels of osteoclast-related genes were suppressed in the L. sakei -treated group.
Conclusion: L. sakei exerted an anti-inflammatory effect in an animal model of RA, regulated Th17 and regulatory B cell differentiation, and suppressed osteoclastogenesis. Our findings suggest that L. sakei has therapeutic potential for RA.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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