Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris

doi:10.21203/rs.3.rs-1729441/v1

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Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris

https://doi.org/10.21203/rs.3.rs-1729441/v1

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Background

The methylotrophic yeast Pichia pastoris is considered as an ideal host for the production of recombinant proteins and chemicals. However, low homologous recombination (HR) efficiency hinders its precise and extensive genetic manipulation. To enhance the homology-directed repair over non-homologous end joining (NHEJ), we expressed five exonucleases that were fused with the Cas9 for enhancing end resection of double strand breaks (DSBs) of DNA cuts.

Results

The endogenous exonuclease Mre11 and Exo1 showed the highest positive rates in seamless deletion of FAA1, and fusing the MRE11 to the C-terminal of CAS9 had the highest positive rate and relatively high number of clones. We observed that expression of CAS9-MRE11 significantly improved positive rates when simultaneously seamless deletion of double genes (from 76.7–86.7%) and three genes (from 10.8–16.7%) when overexpressing RAD52. Furthermore, MRE11 overexpression significantly improved the genomic integration of multi-fragments with higher positive rate and clone number.

Conclusions

Fusion expression of the endogenous exonuclease Mre11 with Cas9 enhances homologous recombination efficiency in P. pastoris. The strategy described here should facilitate the metabolic engineering of P. pastoris toward high-level production of value-added compounds.

Pichia pastoris

CRISPR/Cas9

gene editing

homology-directed repair

metabolic engineering

Pichia pastoris (Komagataella phaffii) is an excellent chassis cell used for protein production and chemicals synthesis [1–4]. In addition, P. pastoris has high potential for sustainable bio-manufacturing utilizing methanol as a substrate, which should be helpful for carbon neutrality [5]. Construction of efficient cell factory requires extensive metabolic rewiring with consequent multiple genes manipulation [6–8]. Currently, the CRISPR/Cas9 system has been successfully applied for genome editing in P. pastoris and other organisms [9–13]. However, the dominant NHEJ over HR in P. pastoris, during the repairing Double Strand Breaks (DSBs) that generated by Cas9 cutting, brings the challenges in precise genetic manipulation such as seamless gene deletion and targeted gene integration (Fig. 1). Recently, we and others tried to engineer the recombination machinery to improve the HR repair process by overexpressing the HR related genes such as RAD52 and/or knocking out the NHEJ genes KU70/80 [10, 11, 14–16]. However, the low efficiency in simultaneous deletion of multiples genes requires further enhancing HR procedure.

The choice of DSB repair pathway between NHEJ and HR mainly depends on the initial processing of the DSBs ends [17, 18]. Generally, initial resection of the HR is trigged by Sae2 protein and the Mre11-Rad50-Xrs2 (MRX) complex in yeast. DSBs end resection is further extended by long-distance exonucleases Exo1 and Dna2 [19]. MRX and other factors work together to generate short 3′ overhangs, which is necessary for initiating HR. Additionally, we previously identified that the nuclease ScSae2 can promote the HR efficiency of GUT1 [16].We thus explored to initiate the exonuclease mediated DNA end resection for enhancing the HR and repressing the NHEJ, and fusing an exonuclease with Cas9 might immediately initiate the formation of 3′ overhangs upon DSBs generation by Cas9 cutting (Fig. 1).

Here, we tried to fuse various exonucleases to Cas9 to enhance the HR process in P. pastoris by accelerating the formation of 3′ overhangs following DSBs generation. With this genetic platform, we improved the simultaneous deletion of multiple genes and integration of multiple fragments, which should be useful for metabolic engineering of P. pastoris and even other non-conventional yeasts.

Enhancing HR efficiency by expressing a fusion of Exonuclease and Cas9 Exonuclease can cleave DSBs to form single-stranded DNA, we thus tried to fuse exonuclease with Cas9 to initiate the HR based repair immediately upon Cas9 cutting. To select the suitable exonuclease, we fused five exonuclease-encoded genes, phage T7 exonuclease (T7Exo), phage λ Red recombination system exonuclease (λRedExo), Escherichia coli exonuclease III (EcExoIII), and endogenous MRE11 and EXO1 to the N-terminal or C-terminal of CAS9 on the plasmids, respectively (Figure S1). We compared the HR efficiency by quantifying the positive rate of seamless deletion of FAA1 (encoding fatty acyl-CoA synthase 1), since FAA1 deletion efficiency was low in wild-type of P. pastoris GS115 [16].

The endogenous MRE11 and EXOI significantly improved the positive rate by 25% and 23.4%, respectively, while the control with only expressing CAS9 had a positive rate of 13.3%. In particular, fusing expression of MRE11 to the C-terminal of CAS9 (MRE11-C) resulted in the highest positive rate of 38.3% (Figure 2A). It is worthy to mention the position of exonuclease resulted in the big variance of positive rates, which might be attributed to difference in enzyme activity or the structure interface between Cas9 and exonuclease. There was a negative correlation between of positive rates and colony forming units (CFU, Figure 2B), which agreed with that HR based repair was rigorous and resulted low survival during DNA repair [16]. Considering the highest positive rate and relative sufficient CFU number, the C-terminal fusion of Mre11 (MRE11-C) to Cas9 was used for further genetic manipulation (Figure 2B).

Synergistic effect of MRE11 and RAD52 for enhancing HR

We previously showed that overexpressing endogenous RAD52 significantly improved HR [16]. We here tried to combine the MRE11 and RAD52 for further enhancing HR. Overexpression of RAD52 had a high positive rate of 88.3% and further fusing the expression of MRE11 to C terminal of CAS9 (CAS9-MRE11) had a higher positive rate of 91.7% when seamlessly deleting FAA1. Consistently, the CFU number of CAS9-MRE11 decreased slightly (Figure 3). We previously showed that RAD52 overexpression significantly improved the manipulation of single genes [16], but precise engineering of multiple genes is still challenging. We thus investigated the synergistic influence of RAD52 overexpression coupled with the MRE11 fusion to Cas9 for simultaneously manipulation of multiple genes.

Simultaneous deletion of multiple genes

We firstly applied our system for simultaneous deletion of multiple genes, which should be helpful for extensive metabolic engineering. Fusion expression of CAS9-MRE11 resulted a high positive rate of 86.7% when simultaneously deleting FAA2 and HFD1, which was higher than that of the control (CAS9, 76.7%) in the RAD52 overexpression strain. Interestingly, CAS9-MRE11 significantly increased the CFU number (Figure 4A). In simultaneous deletion of three genes (FAA2, HFD1 and POX1), CAS9-MRE11 improved the positive rate more significantly (from 10.8% of the control CAS9 to 16.7%) with the similar CFU numbers (Figure 4B). These results suggested that fusing Mre11 with Cas9 helped to initiate the HR based repair of multiple DSBs and thus improved the seamless deletion of multiple genes.

CAS9-MRE11 enhances genomic integration of multi-fragments

Multi-fragments assembly is useful for integrating long biosynthetic pathways. We previously observed that overexpressing RAD52 and deleting MPH1 contributed to the integration of three DNA fragments into specific genomic locus of P. pastoris [16]. We here decided to further investigate if CAS9-MRE11 expression enhance the integration of multi-fragments (total 11 kb) of a fatty alcohol biosynthetic pathway (Figure 5A). In the RAD52 overexpression strain, CAS9-MRE11 improved the positive rate to 91.7%, in comparison to the control strain of 66.7% (Figure 5B). In the RAD52-mph1Δ background, CAS9-MRE11 also resulted a significant higher positive rate of 93.3% compared with the control strain (71.7%). Remarkably, MRE11 overexpression increased CFU number by 103.7% and 76% in these two genetic backgrounds.

The Yeast Srs2 protein is a 3′–5′ DNA helicase and negative regulator of Rad51 function. It removes Rad51 filament and thereby inhibit HR (Figure 5C) [20, 21]. We thus tried to delete SRS2 for enhancing HR, which however resulted in lower positive rates during the integration of multi-fragments. But expressing CAS9-MRE11 still improved the positive rates and CFU numbers in the background RAD52-srs2Δ and RAD52-mph1Δ-srs2Δ (Figure 5B).

We here found that fusing an endogenous exonuclease Mre11 with Cas9 significantly improved the HR process in P. pastoris, which facilitated the genetic manipulation including seamless gene deletion, simultaneous deletion of multiple gene and integration of multiple fragments.

As a component of MRX complex, Mre11 possesses both single-stranded DNA endonuclease activity and 3′-to-5′ exonuclease activity. Mre11 is responsible for short-range resection of DSBs with other HR factors like Sae2/CtIP and directing the repair choice towards HR [18, 19]. Fusion of MRE11 to CAS9 can accelerate recruitment of the other component of MRX to DSBs, which forces Mre11 to occupy the cleavage site and keeps Ku70/80 dimer away from DSBs (Fig. 1). Thus, this strategy can help to enhance HR process without disrupting NHEJ procedure. Previous studies showed that interfering the NHEJ pathway, especially deletion of KU70Δ, resulted in genetic instability of chromosome [16], sensitive to UV rays, negative effect on the cell growth and production [22–24]. Furthermore, methanol biotransformation in P. pastoris will suffers the methanol toxicity that might bring chromosome damage and the NHEJ pathway can help cells restore their viability rapidly. Thus, the strategy described here will not cause potential adverse effects on cellular fitness and will be helpful for constructing robust cell factory.

We and others previously showed that overexpression of HR machinery genes such as RAD52 remarkably enhance the HR efficiency in yeast and mammalian cells [14] [16] [25, 26], which however resulted in low colony numbers and brought the difficulties in picking out the positive clones. One possible reason is that HR is considerably slower than NHEJ and the NHEJ can save more cells from DSBs event. NHEJ requires little or no end resection to directly reconnect the DSB ends. In contrast, HR requires short resection and long resection of DSBs and the donor to implement the repair process. In addition, other proteins are also likely to be limiting factors in the HR repair pathway [18, 19]. We here found that fusing MRE11 with CAS9 improved the CFU numbers during simultaneous deletion of two genes and integration of multi-fragments (Fig. 4A and 5), suggesting that Mre11 is conducive to initiate the HR based repair of multiple DSBs rapidly. Improving CFU number should be helpful for picking out the positive clones during multiplex gene editing and pathway construction. These results also suggested that MRE11 and RAD52 had a synergy in enhancing HR in P. pastoris.

D-loops are vital intermediates of HR. Most nascent D-loops are impaired by two pathways: one mediated by the Srs2 helicase and the other involved in the Mph1 helicase and the Sgs1-Top3-Rmi1 complex [27]. SRS2 deletion does not give rise to increase of HR efficiency, which might be attributed to the complex regulation of cell multi-invasion-induced rearrangement process [28]. Finally, we anticipated that elucidating an explicit role of protein Srs2 will favor regulation of HR in further applications.

In conclusion, we show that fusing the endogenous MRE11 with CAS9 enhance HR efficiency in P. pastoris, which enhanced precise manipulation of multiple genes and might be applied to other non-conventional microbes.

Strains, Media, and Cultivation Conditions

P. pastoris GS115 and its derived strains were cultivated in YPD medium (20 g/L glucose, 10 g/L yeast extract and 20 g/L peptone) at 30ºC. For screening of transformants, YPD medium was supplemented with 100 mg/L zeocin. Escherichia coli DH5α was grown in LB medium (10 g/L tryptone, 10 g/L NaCl, and 5 g/L yeast extract) at 37ºC and used for plasmid construction. 25 mg/L zeocin was added to select and maintain plasmids. All strains used in this study are listed in the Supplementary Information Table S1.

Plasmids Construction

All plasmids and primers used in this study were listed in the Supplementary Information Table S2 and Table S3. Plasmid construction was performed as previously described [16]. Most gRNA expression plasmids were constructed based on pPICZ-Cas9-gFAA1, which includes a CAS9 gene and HH-FAA1-gRNA-HDV. The genes encoding phage T7 exonuclease (T7Exo), phage λ Red system exonuclease (λRedExo) Escherichia coli exonuclease III (EcExoIII) were codon optimized and synthesized by GENEWIZ (Suzhou, China) (Supplementary Information Table S4). Two endogenous genes MRE11 and EXO1 were obtained by PCR. These exonuclease genes were fused to the N or C-terminus of CAS9 gene with a linker (GGGGS)₃. For gRNA expression plasmids, three fragments including vector backbone, CAS9 and the exonuclease genes were ligated with ClonExpress II One Step Cloning Kit (Vazyme Biotech Co., Ltd, Nanjing, China).

Construction of knockout strains

FAA1, FAA2 and SRS2 gene was seamlessly deleted by the gRNA plasmid pPICZ-Cas9-gFAA1, pPICZ-Cas9-gFAA2, pPICZ-Cas9-gSRS2 and the corresponding donor, respectively. The plasmid pPICZ-Cas9-gSRS2 was constructed with primers SRS2-sgRNA-F/Backbone-DR and SV40-DF/SRS2-sgRNA-R based on pPICZ-Cas9-gFAA1. The upstream and downstream homologous arms (HAs) with a length of 1000 bp were amplified from genome, and then were fused by overlap extension PCR to form a donor for seamless deletion.

DNA transformation was carried out with a condensed electroporation protocol [29], and the transformed cells were grown for three to four days on YPD plates containing 100 mg/mL Zeocin. Twenty colonies were randomly picked from each plate for identification of positive clones unless otherwise noted. The deletion and integration manipulations in the genome were confirmed with colony PCR, and the genomic DNA was extracted according to a described protocol [30]. The positive rate was calculated by dividing the number of correct colonies by the total number of picked colonies.

Multi-fragment integration at the FAA1 gene site

A fatty alcohol biosynthetic pathway of 11 kb was divided into three expression cassettes. MmCAR, npgA and ADH5 gene was driven by promoters P_ADH2, P_TEF1 and P_TPI1, respectively. The HAs length at both ends was 1 000 bp, and the other two HAs were about 500 bp (Figure 5A). These gene expression cassettes were assembled by overlap extension PCR, and simultaneously integrated into FAA1 site by using the gRNA plasmid pPICZ-Cas9-gFAA1 or pPICZ-Cas9-PpMre11-gFAA1. 500 ng each double-stranded DNA expression cassette as donors and 500 ng plasmids were transformed to yeast cells.

homologous recombination

DSBs

double strand breaks

NHEJ

non-homologous end joining

CFU

colony forming units

MRX

Mre11-Rad50-Xrs2.

Supplementary Information

The online version contains supplementary material available at xxx

Author contributions

Y.J.Z. conceived the study; K.Z., X.D.P. and P.C. designed the experiments; K.Z. performed the experiments and analyzed the data; L.H.G. and X.Y.W assisted with the genomic DNA extraction; K.Z. wrote the first draft; P.C., L.Y. and Y.J.Z. revised the manuscript; all authors approved the manuscript.

Notes

The authors applied a patent based on the design and method described in this study.

Funding

This work was financially supported National Key Research and Development Program of China (2021YFC2100500), National Natural Science Foundation of China (22161142008 and 21922812), and DICP innovation grant (DICP I201920) from Dalian Institute of Chemicals Physics, CAS.

Author details

¹ Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; ² Henan Engineering Laboratory for Bioconversion Technology of Functional microbes, College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, China; ³ College of Life Sciences, Liaoning Normal University, Dalian 116029, Liaoning, China; ⁴ School of Bioengineering, Dalian University of Technology, Dalian 116024, China; ⁵University of Chinese Academy of Sciences, Beijing 100049, China；⁶ Dalian Key Laboratory of Energy Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; ⁷CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

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No competing interests reported.

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Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris

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