Postoperative C-reactive protein kinetics predict postoperative complications in patients treated with cytoreductive surgery and hyperthermic intraperitoneal chemotherapy for peritoneal carcinomatosis

DOI: https://doi.org/10.21203/rs.3.rs-17404/v1

Abstract

Background Relatively high morbidity rates are reported after cytoreductive surgery (CRS) with hyperthermic intraperitoneal chemotherapy (HIPEC). However, early predictors of complications after CRS plus HIPEC have not been identified. The aim of this study was to evaluate the predictive role of early postoperative serum C-reactive protein (CRP) level (Day 2-4) for the detection of post-operative complications.

Patients and methods We performed a retrospective study including 94 patients treated with complete CRS (R1) and HIPEC for PC from various primary origins (2011-2016). Post-operative complications were recorded. The values for postoperative inflammatory markers (white blood cells [WBC] and platelet counts, CRP) were compared between the different groups.

Results CRP on post-operative days 2-4 was significantly higher in patients with than without complications (124 mg/L vs 46 mg/L; p<0.0001) and higher in those with more major complications (162 mg/L vs 80 mg/L; p< 0.0012). WBC and platelet counts showed no difference within 5 days postoperatively.

Conclusion CRP levels, and kinetics mainly, between post-operative day 2 and 4, are decisive predictive markers of early and late post-operative complications after CRS plus HIPEC. The presence of post-operative complications should be suspected in patients with a high CRP mean, and a plateau level (days 2-4).

Introduction

The use of cytoreductive surgery (CRS) with hyperthermic intraperitoneal chemotherapy (HIPEC) is becoming more and more widespread in the treatment of peritoneal carcinomatosis (PC) in multiple types of cancer [1, 2, 3].

Patients with peritoneal carcinomatosis have a dismal prognosis, irrespective of the primary tumor site, with a median survival of 6 months in the absence of treatment [4]. An estimated 66% of PCs arise from gastrointestinal (GI) tumors (colorectal (60%), gastric (20%), and pancreatic (20%) and 33% arise from tumors of other origins, including ovarian tumors in more than 50% of cases [5]. CRS with or without HIPEC has been reported to be beneficial in terms of outcomes in patients treated for PC from various origins including gastric, ovarian, pseudomyxoma peritonei (PMP), and colorectal cancer, with a median survival of 42 months in patients with PC from colorectal origin, versus 15 months for chemotherapy-only patients, and a 5-year survival of 34.5% [6, 7, 8, 9].

However, CRS alone or combined with HIPEC may be accompanied by postoperative complications in 30–60% of cases [8, 9, 10, 11], these being mainly related to the extent of CRS, the general condition of the patient, and the experience of the center [12].

Early detection of postoperative complications is important to avoid delays in management. However, in patients treated with CRS and HIPEC, the clinical detection of these complications is not always easy [13]. C-reactive protein (CRP) has been reported as a potential tool for predicting postoperative complications [14, 15, 16]. The normal level of CRP is less than 0.8 mg/L and varies beyond this limit during an inflammatory event. In general, the concentration doubles every 8 h and reaches its maximum value after 36–50 h [17]. The intensity of the inflammatory event influences the amount of CRP produced [18]. Very few data are available regarding the value of CRP measurement in patients treated with CRS and HIPEC [19].

The primary objective of this study is to evaluate whether postoperative CRP levels are predictive of postoperative complications in patients treated for PC with CRS and HIPEC.

Methods

We performed a retrospective monocentric study, including patients treated with curative intent, with R0/R1 resections plus CRS and HIPEC, for PC of colorectal, appendicular, ovarian, and gastric cancers, between January 2011 and December 2016.

Data Retrieval

Patient characteristics, type of surgery performed, postoperative complications, CRP and WBC values were collected from a prospective database on patients treated with CRS and HIPEC and from the institution’s program (Oribase).

Complications

Post-operative complications were recorded until postoperative day (POD) 30, and classified according to Clavien-Dindo classification (CDC).

Patients were categorized into two main groups: those who developed postoperative complications versus those who didn’t. Those who developed complications were further subdivided into subgroups, according to A- The severity of their complications: minor (I and II) versus major (III and IV), using the CD classification, and B- The nature of their complications: infectious versus non-infectious.

Inflammatory markers

Patients receiving CRS with HIPEC are subject to preoperative laboratory work-up, including CRP levels, white blood cell (WBC) and platelet counts, and daily biomarker control during their stay in the intensive care unit (POD 0 to POD 7), including CRP levels, WBC and platelet counts. The values of postoperative inflammatory markers: WBC count, platelet count, and CRP levels, were compared between the different groups.

Statistical analysis

CRP means and medians were calculated for the first seven days postoperatively, and the CRP average was calculated, for both, main groups and subgroups, from POD 2 until POD 4. Student’s t-test was used for the analysis and comparison of CRP averages between the two main groups (with versus without complications), and between the two subgroups (minor versus major complications). An ANOVA parametric test was used as well, to compare between patients without complications and subgroups A & B. Whenever this test revealed significant associations, a Chi-square test was done to confirm the independence of the variable.

To evaluate the predictive value of postoperative CRP on the occurrence of complications, we also calculated the Positive Predictive Value (PPV) and Negative Predictive Value (NPV), Sensitivity (Se), and specificity (Spe) of the test. The CRP thresholds used for the measurements of PPV, NPV, Se, and Spe were the p50 (median) and the p25.

The effect of other inflammatory markers (WBC and platelets), on the occurrence of postoperative complications, was evaluated by calculating their averages among patients with and without postoperative complications.

Results

A total of 94 patients were included, 66 females and 28 males. The mean age was 55 years and 6 months (median 57 years, range: 31–75). Fifty-five patients had peritoneal carcinomatosis of colorectal origin, 18 of ovarian origin, 14 of appendiceal origin, 5 of gastric origin, and 2 patients had malignant mesothelioma (Table I). The average PCI was 10.74 (median = 8, range: 0–35).

Sixty-six out of 94 patients (70.21%) developed at least 1 postoperative complication through POD 30. Table II provides details on the type of complications, and the number and proportion of each complication in relation to all the complications recorded in the 94 individuals. A total of 137 complications were classified under 13 categories according the CD scoring system. Thirty-nine out of 94 patients (41.49%) had a grade II complication (Table II).

The highest rate of postoperative complications was detected in Gastric cancers (80%), followed by Pseudomyxoma Peritonei (79%) then Colorectal (71%) and Ovarian (61%). Furthermore, the higher the PCI, the higher was the complication rate: 84% in PCIs > 17, 73% in PCIs between 7 and 16, and 62% in PCIs < 6.

Mean CRP on POD 2–4 (Table III) was significantly lower for patients without complications compared to that of patients with complications (45.79 mg/L and 124.3 mg/L, respectively, p < 0.0001). Mean CRP on POD 2–4 for patients with minor complications was also significantly lower than that of patients with major complications (80.06 mg/L and 162.4 mg/L, respectively, p = 0.0012). CRP values on POD 2–4, were, therefore, higher in patients with complications, particularly for those with grade III-IV complications. The sensitivity, specificity, PPV, and NPV of postoperative CRP levels for the prediction of postoperative complications were, respectively, 85.25%, 46.17%, 78.79%, 57.14% (Table III.

When compared, WBC and platelets levels did not show statistical significance, in predicting whether the patient will develop a complication, particularly between POD 2–4.

Discussion

Early postoperative (POD 2–4) CRP levels of patients treated for PCs from different origins by cytoreductive surgery and HIPEC predict postoperative infectious and non-infectious complications. Conversely, WBC and platelet counts are not predictive of postoperative complications in these patients.

The morbidity rate of patients treated with CRS and HIPEC for PC reported in the literature is around 30%-60% [8]. Ours was around 70%, including the CD I rated complications. Our study showed as well a higher rate of postoperative complications in gastric cancer, pseudomyxoma peritonei and high PCIs, correlating with available literature data [20].

In practice, the diagnosis of early post-operative complications in patients who have undergone multiple intestinal resections and anastomoses, multiple organ resections, peritonectomies, or diaphragmatic resection is often difficult. A biological marker that could allow for earlier detection of complications, and thus, an earlier management of patients likely to develop postoperative complications, would be a very useful tool for improving the management of these patients.

CRP is a commonly used biological marker, being accurate, inexpensive, and easy to measure. CRP levels reflect the intensity of an inflammatory reaction, and CRP also participates in activating the inflammatory cascade [8]. Recent studies have shown its efficacy for predicting postoperative complications, particularly in abdominal surgery [16, 21].

However, its usefulness in the setting of CRS combined with HIPEC has not been determined yet. To our knowledge, only one study has focused on the value of CRP for predicting postoperative complications of patients treated with CRS and HIPEC [11]. Fernandez et al. showed that CRP levels at POD 2 were highly associated with postoperative early infectious complications in patients who underwent CRS plus HIPEC for PC of ovarian origin [22]. However, they did not prove its significance in predicting non-infectious complications or late infectious ones.

In our study, we observed that CRP levels were higher in patients who developed postoperative complications whether infectious or not (Table I). Moreover, higher CRP levels reflected a higher complication grade (Table III). Also, we would like to emphasize that the early kinetics of CRP levels, rather than the actual CRP value, is more important. The sensitivity of detecting complications using CRP kinetics between POD 2 & 4 was 85%, with a specificity of 45%, PPV of 79%, and NPV of 57%. As shown in Fig. 1, the patients who were more prone to develop postoperative complications were those in whom CRP levels tended to rise and maintain a certain plateau between POD 2 and 4. No matter the actual peak in the CRP value, it was the persistent plateau that defined or predicted the imminent complication reflected by the mean at POD 2–4. The study of other inflammatory markers (WBC and platelets) on the occurrence of postoperative complications did not demonstrate any statistical significance between patients with and without complications.

One of the weaknesses of our study was its retrospective design. However, measurements for biological inflammatory markers (CRP, platelets, and WBCs) were performed in a systematic way and complications were reported in a prospective database. The major limitation related to the study design was that we evaluated a predictor of complications (CRP) over a shorter period of time (7 postoperative days) compared to the occurrence of the complications themselves (1 month). Despite this, the results remain highly significant because the majority of complications appeared in the course of the first postoperative week.

Conclusions

The risk of postoperative complications related to cytoreductive surgery associated with HIPEC in patients treated for PC remains significant and diagnosis is often difficult. We report that the kinetics of CRP level, expressed as mean POD 2-4 CRP level, is predictive of postoperative complications in patients treated with CRS plus HIPEC. Patients should be monitored to identify those with persistently high CRP levels expressed as mean POD 2-4 CRP levels.

Declarations

Ethics approval and consent to participate

Ethical approval was obtained from our institutional ethical committee board: Jules Bordet Ethical committee.

Consent for publication

Not applicable.

Availability of data and materials

The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.

Competing interests

The authors declare that they have no competing interests.

Funding

The authors have no funding source to disclose for this work.

Authors' contributions

The authors contributed equally to this manuscript.

Acknowledgements

We acknowledge the contribution of a medical writer, Sandy Field, PhD, for English language editing and formatting of this manuscript.

 

References

  1. Kaminsky MC, Fuk D, Marchal F, Guillemin F, Meistelman C. Prise en charge des carcinomatoses péritonéales d’origine digestive: chirurgie de cytoréduction et chimiothérapie intrapéritonéale. Cancéro digest. 2010 Mar 12.
  2. Coccolini F, Celotti A, Ceresoli M, Montori G, Fugazzola P, Ansaloni L. Hyperthermic intraperitoneal chemotherapy (HIPEC) and neoadjuvant chemotherapy as prophylaxis of peritoneal carcinosis from advanced gastric cancer—Effects on overall and disease free survival. European Journal of Surgical Oncology. 2018 Apr 1;44(4):547.
  3. Vaira M, Robella M, Cinquegrana A, De Simone M. Treatment of peritoneal carcinomatosis from ovarian cancer by surgical cytoreduction and Hyperthermic Intraperitoneal Chemotherapy (HIPEC). European Journal of Surgical Oncology. 2016 Oct 1;42(10):S214-5.
  4. Franko J, Shi Q, Goldman CD, Pockaj BA, Nelson GD, Goldberg RM, Pitot HC, Grothey A, Alberts SR, Sargent DJ. Treatment of colorectal peritoneal carcinomatosis with systemic chemotherapy: a pooled analysis of north central cancer treatment group phase III trials N9741 and N9841. Journal of Clinical Oncology. 2012 Jan 20;30(3):263.
  5. Elias D. Traitement combiné par chirurgie et chimiothérapie locale des carcinoses péritonéales d’origine digestive. Hépato-Gastro & Oncologie Digestive. 1999 Dec 6;6(6):429-36.
  6. Tentes AA, Tripsiannis G, Markakidis SK, Karanikiotis CN, Tzegas G, Georgiadis G, Avgidou K. Peritoneal cancer index: a prognostic indicator of survival in advanced ovarian cancer. European Journal of Surgical Oncology (EJSO). 2003 Feb 1;29(1):69-73.
  7. Portilla AG, Sugarbaker PH, Chang D. Second-look surgery after cytoreduction and intraperitoneal chemotherapy for peritoneal carcinomatosis from colorectal cancer: analysis of prognostic features. World journal of surgery. 1999 Jan 1;23(1):23-9.
  8. Lheureux S, Gourley C, Vergote I, Oza AM. Epithelial ovarian cancer. The Lancet. 2019 Mar 23;393(10177):1240-53.
  9. Huang Y, Alzahrani NA, Chua TC, Liauw W, Morris DL. Impacts of peritoneal cancer index on the survival outcomes of patients with colorectal peritoneal carcinomatosis. International Journal of Surgery. 2016 Aug 1;32:65-70.
  10. Saxena A, Yan TD, Morris DL. A critical evaluation of risk factors for complications after cytoreductive surgery and perioperative intraperitoneal chemotherapy for colorectal peritoneal carcinomatosis. World journal of surgery. 2010 Jan 1;34(1):70-8.
  11. Liberale G, Sugarbaker PH. Sclerosing encapsulating peritonitis as a potential complication of cytoreductive surgery and HIPEC: Clinical features and results of treatment in 4 patients. Surgical oncology. 2018 Dec 1;27(4):657-62.
  12. Elias D, Gilly F, Boutitie F, Quenet F, Bereder JM, Mansvelt B, Lorimier G, Dube P, Glehen O. Peritoneal colorectal carcinomatosis treated with surgery and perioperative intraperitoneal chemotherapy: retrospective analysis of 523 patients from a multicentric French study. J Clin Oncol. 2010 Jan 1;28(1):63-8.
  13. Baratti D, Kusamura S, Mingrone E, Balestra MR, Laterza B, Deraco M. Identification of a subgroup of patients at highest risk for complications after surgical cytoreduction and hyperthermic intraperitoneal chemotherapy. Annals of surgery. 2012 Aug 1;256(2):334-41.
  14. Welsch T, Frommhold K, Hinz U, Weigand MA, Kleeff J, Friess H, Büchler MW, Schmidt J. Persisting elevation of C-reactive protein after pancreatic resections can indicate developing inflammatory complications. Surgery. 2008 Jan 1;143(1):20-8.
  15. Welsch T, Müller SA, Ulrich A, Kischlat A, Hinz U, Kienle P, Büchler MW, Schmidt J, Schmied BM. C-reactive protein as early predictor for infectious postoperative complications in rectal surgery. International journal of colorectal disease. 2007 Dec 1;22(12):1499-507.
  16. Pedrazzani C, Moro M, Mantovani G, Lazzarini E, Conci S, Ruzzenente A, Lippi G, Guglielmi A. C-reactive protein as early predictor of complications after minimally invasive colorectal resection. Journal of Surgical Research. 2017 Apr 1;210:261-8.
  17. Adukauskienė D, Čiginskienė A, Adukauskaitė A, Pentiokinienė D, Šlapikas R, Čeponienė I. Clinical relevance of high sensitivity C-reactive protein in cardiology. Medicina. 2016 Feb;52(1):1-0.
  18. Elias D, Raynard B, Farkhondeh F, Goéré D, Rouquie D, Ciuchendea R, Pocard M, Ducreux M. Peritoneal carcinomatosis of colorectal origin: long-term results of intraperitoneal chemohyperthermia with oxaliplatin following complete cytoreductive surgery. Gastroenterologie clinique et biologique. 2006 Oct 1;30(10):1200-4.
  19. D’Hondt V, Goffin F, Roca L, Dresse D, Leroy C, Kerger J, Cordier L, De Forges H, Veys I, Liberale G. Interval cytoreductive surgery and hyperthermic intraperitoneal chemotherapy in first-line treatment for advanced ovarian carcinoma: a feasibility study. International Journal of Gynecologic Cancer. 2016 Jun 1;26(5):912-7.
  20. Bakrin N, Classe JM, Pomel C, Gouy S, Chene G, Glehen O. La chimiohyperthermie intrapéritonéale (CHIP) dans les cancers ovariens. Journal de Chirurgie Viscérale. 2014 Oct 1;151(5):358-65.
  21. Almeida AB, Faria G, Moreira H, Pinto-de-Sousa J, Correia-da-Silva P, Maia JC. Elevated serum C-reactive protein as a predictive factor for anastomotic leakage in colorectal surgery. International Journal of Surgery. 2012 Jan 1;10(2):87-91.
  22. Fernández FM, Munoz-Casares FC, Arjona-Sanchez A, Casado-Adam A, Gomez-Luque I, Arismendi DG, Thoelecke H, Peña SR, Delgado JB. Postoperative time course and utility of inflammatory markers in patients with ovarian peritoneal carcinomatosis treated with neoadjuvant chemotherapy, cytoreductive surgery, and HIPEC. Annals of surgical oncology. 2015 Apr 1;22(4):1332-40.

Tables

Table I: Patient characteristics, with and without post-operative complications.

 

Total N of patients
 N= 94 (100%)

Without complications
 N= 28 (29.8%)

With complications

N= 66 (70.2%)

 

 

 

 

Age (years)                         

                Mean                                
              Median

 

55.5  
 57

 

54 

54 

 

56.2

58 

Gender                             

Males                        
              Females


28 (29.8%)
 66 (70.2%)


8 (28.6%)
 20 (71.5%)


20 (30.3%)
 46 (69.7%)

  Primary cancer

Colorectal                      
 Ovarian

Pseudomyxoma                        

Gastric

Mesothelioma               

 

 
 55 (58.5%)

 18 (19.2%)

 14 (14.9%) 

   5 (5.3%)

   2 (2.1%)


16 (57.1%)
7 (25%)
3 (10.7%)
1 (3.6%)
 1 (3.6%)


39 (59.1%)
11 (16.7%)
11 (16.7%)
4 (6%)
1 (1.5%) [LG1] 

PCI

                           <6
7-12
                      13-16

                        >17
        Undetermined

 

39 (41.5%)
 19 (20.2%)

  7 (7.5%)
25 (26.6%)
   4 (4.2%)

 

15 (53.6%)
5 (17.9%)
2 (7.1%)
4 (14.3%)
   2 (7.1%)

 

24 (36.4%)
14 (21.2%)
5 (7.6%)
21 (31.8%)
2 (3%) [LG2] 

Surgical Intervention

Colorectal resection                      
Enterostomy
Gastrectomy
Cholecystectomy
Appendectomy
Omentectomy
Peritonectomy
Splenectomy
Oophorectomy/ Hysterectomy
Lombo-aortic lymphadenectomy
 Diaphragmatic resection

   

 65 (69.1%)
35 (37.2%)
10 (10.6%)
60 (63.8%)
23 (24.5%)
66 (70.2%)
56 (59.6%)
27 (28.7%)
36 (38.3%)
       15 (16%)        

30 (32%)

 

  

    17 (60.7%)
5 (17.9%)
2 (7.1%)
15 (53.6%)
5 (17.9%)
20 (71.4%)
14 (50%)
7 (25%)
13 (46.4%)
        5 (18%)      

 7 (25%)

      

48 (72.7%)
29 (43.9%)
8 (12.1%)
45 (68.2%)
18 (27.3%)
45 (68.2%)
42 (63.6%)
20 (30.3%)
23 (34.9%)
      10 (15.2%)     

21 (31.8%)

     

 


 

Table II: Clavien-Dindo classification of complications in all patients.

 

N (%)

Grade 1

Grade 2

Grade 3a

Grade 3b

Grade 4a

Grade 4b

Grade 5

Anastomotic leak

4 (2.92%)

2 (50%)

 

 

2 (50%)

 

 

 

Peritonitis

15 (10.94%)


12 (80%)

2 (13.33%)

1 (6.67%)

 

 

 

Hemoperitoneum

5 (3.65%)

1 (20%)

 

1 (20%)

3 (60%)

 

 

 

Small bowel obstruction

3 (2.19%)

 

1 (33.33%)

 

2 (66.67%)

 

 

 

Urinary tract infection

35 (25.55%)

1 (2.86%)

32 (91.42%)

1 (2.86%)

1 (2.86%)

 

 

 

Septicemia

14 (10.22%)


11 (78.57%)

 

 


2 (14.29%)

1 (7.14%)

Pancreatitis

4 (2.92%)

2 (50%)

1 (25%)

 

1 (25%)

 

 

 

Cerebro-vascular accident

3 (2.19%)

1 (33.33%)

 

 

 

 

 

2 (66.67%)

Pulmonary complications

27 (19.71%)

4 (14.81%)

12 (44.44%)

3 (11.11%)

 

7 (25.94%)

 

1 (3.70%)

Cardio-vascular complications

8 (5.84%)

 

7 (87.5%)

 

 

1 (12.5%)

 

 

Renal failure

11 (8.03%)

5 (45.45%)

5 (45.45%)

1 (9.10%)

 

 

 

 

Wound infection

8 (5.84%)

4 (50%)

3 (37.5%)

1 (12.5%)

 

 

 

 

Total number of complications

137

20 (14.60%)

84 (61.31%)

9 (6.57%)

10 (7.30%)

8 (5.84%)

2 (1.46%)

4 (2.92%)

Percentage of each complication in 94 patients

2.13%

41.49%

6.38%

10.64%

5.32%

1.06%

3.19%

 



Table 3
 Patients Characteristics

WBC Mean between POD 2 and POD 4

p-value

 

CRP Mean between POD 2 and POD 4

p-value

p50

p25

Without Complications

vs

With Complications

8.23

 

9.46

 

p > 0,05

 

45,79

 

124,3

 

p< 0,0001

Se: 65.57%

Spe: 46.15%

PPV:67.44%

NPV:66.67%

Se: 85.25%

Spe: 46.17%

PPV: 78.79%

NPV: 57.14%

CD 1-2

vs

CD 3-4

N/A

N/A

 

80,06

 

162,4

 

p= 0,0012

Se: 64.41%

Spe: 78.51%

PPV: 86.36%

NPV: 51.16%

Se: 84.75%

Spe: 57.14%

PPV: 75.76%

NPV: 57.14%

Without Complication

vs

CD 1-2

vs

CD 3-4

N/A

 

N/A

 

45,79

 

80,06

 

162,4

 

 

p< 0,0001

 

Se: 57.14%

Spe: 84.62%

PPV: 16.67%

NPV: 59.46%

 

Se: 82.86%

Spe: 46.15%

PPV: 67.44%

NPV: 66.67%

Without Complications

vs

Infectious Complications

8.23

 

9.2

 

p > 0,05

 

45,79

 

107,0

 

p= 0,0004

N/A

N/A

Without Complications

vs

Non-Infectious Complications

8.23

 

9.8

 

p > 0,05

 

45,79

 

107,5

 

p=0,0163

N/A

N/A