Growth hormone alleviates oxidative stress and improves IVF outcomes in Chinese women with poor ovarian response: A randomized controlled trial
Background Oxidative stress (OS), defined as an imbalance between reactive oxygen species (ROS) and/or reactive nitrogen species (RNS) excessive production and antioxidant insufficient, has been suggested to be involved in the pathogenesis of poor ovarian response (POR). Growth hormone (GH) can function reduce OS in some types of cells. This study investigated whether GH can significantly improve OS and in vitro fertilization and embryo transfer (IVF-ET) outcomes in patients with POR.
Methods This study enrolled 105 and 58 patients with and without POR (controls), respectively, diagnosed according to the Bologna criteria, who underwent conventional IVF-ET. Patients with POR were randomly assigned to two groups: POR-GH group: pretreatment with GH 4IU/d on day 2 of the previous menstrual cycle before IVF till the trigger day; POR-C group: no pretreatment. The markers of OS in follicle fluid (FF),reactive oxygen species (ROS) levels in granulosa cells (GC), and IVF outcomes of the patients were compared between the three groups.
Result(s) The endometrial thickness on trigger day, number of cleaved embryos, higher quality embryos, the rates of implantation and clinical pregnancy were significantly increased in POR-GH group compared with POR-C group (P<0.05). Moreover, the FF malondialdehyde (MDA), total oxidant status (TOS), oxidative stress index (OSI) and ROS levels in GC were significantly higher,whereas superoxide dismutase (SOD) and total antioxidant capacity (TAC) were significantly lower in POR-C group compared with non-POR group (P<0.05). Furthermore, the FF TAC was significantly increased, whereas TOS, OSI and intracellular ROS levels were significantly reduced in POR-GH group compared with POR-C group (P<0.05).
Conclusion(s) Pretreatment with GH alleviated OS and improved oocyte quality and IVF outcomes in patients with POR.
Clinical Trial Registration: Chinese Clinical Trial Registry. ChiCTR1900021269. Registered 8 February 2019, http://www.chictr.org.cn/edit.aspx?pid=35837&htm=4.
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Growth hormone alleviates oxidative stress and improves IVF outcomes in Chinese women with poor ovarian response: A randomized controlled trial
On 05 Sep, 2020
On 23 Aug, 2020
On 16 Aug, 2020
On 15 Aug, 2020
On 15 Aug, 2020
On 12 Aug, 2020
Received 03 Aug, 2020
On 29 Jul, 2020
Invitations sent on 26 Jul, 2020
On 08 Jul, 2020
On 07 Jul, 2020
On 07 Jul, 2020
On 21 Jun, 2020
Received 19 Jun, 2020
On 15 Jun, 2020
Invitations sent on 14 Jun, 2020
On 24 May, 2020
On 23 May, 2020
On 23 May, 2020
Posted 17 Mar, 2020
On 03 May, 2020
Received 21 Apr, 2020
On 16 Apr, 2020
Invitations sent on 06 Apr, 2020
On 13 Mar, 2020
On 12 Mar, 2020
On 12 Mar, 2020
On 12 Mar, 2020
Background Oxidative stress (OS), defined as an imbalance between reactive oxygen species (ROS) and/or reactive nitrogen species (RNS) excessive production and antioxidant insufficient, has been suggested to be involved in the pathogenesis of poor ovarian response (POR). Growth hormone (GH) can function reduce OS in some types of cells. This study investigated whether GH can significantly improve OS and in vitro fertilization and embryo transfer (IVF-ET) outcomes in patients with POR.
Methods This study enrolled 105 and 58 patients with and without POR (controls), respectively, diagnosed according to the Bologna criteria, who underwent conventional IVF-ET. Patients with POR were randomly assigned to two groups: POR-GH group: pretreatment with GH 4IU/d on day 2 of the previous menstrual cycle before IVF till the trigger day; POR-C group: no pretreatment. The markers of OS in follicle fluid (FF),reactive oxygen species (ROS) levels in granulosa cells (GC), and IVF outcomes of the patients were compared between the three groups.
Result(s) The endometrial thickness on trigger day, number of cleaved embryos, higher quality embryos, the rates of implantation and clinical pregnancy were significantly increased in POR-GH group compared with POR-C group (P<0.05). Moreover, the FF malondialdehyde (MDA), total oxidant status (TOS), oxidative stress index (OSI) and ROS levels in GC were significantly higher,whereas superoxide dismutase (SOD) and total antioxidant capacity (TAC) were significantly lower in POR-C group compared with non-POR group (P<0.05). Furthermore, the FF TAC was significantly increased, whereas TOS, OSI and intracellular ROS levels were significantly reduced in POR-GH group compared with POR-C group (P<0.05).
Conclusion(s) Pretreatment with GH alleviated OS and improved oocyte quality and IVF outcomes in patients with POR.
Clinical Trial Registration: Chinese Clinical Trial Registry. ChiCTR1900021269. Registered 8 February 2019, http://www.chictr.org.cn/edit.aspx?pid=35837&htm=4.
Figure 1
Figure 2