Background Archaea are the least-studied members of the gut-dwelling autochthonous microbiota. Few studies have reported the exclusive dominance of methanogens in the archaeal microbiome (archaeome) of the human gut, although information regarding the diversity and abundance of other archaeal phylotypes is limited.
Results We surveyed the archaeome in the faecal samples collected from 897 normal East Asian subjects living in South Korea. In total, 42.47% faecal samples were positive for archaeal colonization, which were subsequently subjected to archaeal 16S rRNA gene deep sequencing and abundance estimation. The mean archaeal abundance was 9.89 ± 4.48% of the total bacterial and archaeal abundance. We observed extensive colonization of haloarchaea (95.53%) in the Korean gut, with 9.63% mean relative abundance in archaeal communities. Haloarchaea were relatively more abundant than methanogens in certain samples. The presence of haloarchaea was also verified by fluorescence in situ hybridization analysis. Owing to large inter-individual variations, we categorized the human gut archaeome into four archaeal enterotypes.
Conclusions The study demonstrated that the human gut archaeome is indigenous, responsive, and functional, expanding our understanding of the archaeal signature in the gut of normal healthy individuals.
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On 04 Aug, 2020
On 02 Jun, 2020
On 01 Jun, 2020
On 01 Jun, 2020
Posted 17 Mar, 2020
On 04 May, 2020
Received 28 Apr, 2020
Received 24 Apr, 2020
On 28 Mar, 2020
On 26 Mar, 2020
Invitations sent on 25 Mar, 2020
On 13 Mar, 2020
On 12 Mar, 2020
On 12 Mar, 2020
On 12 Mar, 2020
On 04 Aug, 2020
On 02 Jun, 2020
On 01 Jun, 2020
On 01 Jun, 2020
Posted 17 Mar, 2020
On 04 May, 2020
Received 28 Apr, 2020
Received 24 Apr, 2020
On 28 Mar, 2020
On 26 Mar, 2020
Invitations sent on 25 Mar, 2020
On 13 Mar, 2020
On 12 Mar, 2020
On 12 Mar, 2020
On 12 Mar, 2020
Background Archaea are the least-studied members of the gut-dwelling autochthonous microbiota. Few studies have reported the exclusive dominance of methanogens in the archaeal microbiome (archaeome) of the human gut, although information regarding the diversity and abundance of other archaeal phylotypes is limited.
Results We surveyed the archaeome in the faecal samples collected from 897 normal East Asian subjects living in South Korea. In total, 42.47% faecal samples were positive for archaeal colonization, which were subsequently subjected to archaeal 16S rRNA gene deep sequencing and abundance estimation. The mean archaeal abundance was 9.89 ± 4.48% of the total bacterial and archaeal abundance. We observed extensive colonization of haloarchaea (95.53%) in the Korean gut, with 9.63% mean relative abundance in archaeal communities. Haloarchaea were relatively more abundant than methanogens in certain samples. The presence of haloarchaea was also verified by fluorescence in situ hybridization analysis. Owing to large inter-individual variations, we categorized the human gut archaeome into four archaeal enterotypes.
Conclusions The study demonstrated that the human gut archaeome is indigenous, responsive, and functional, expanding our understanding of the archaeal signature in the gut of normal healthy individuals.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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