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Research
Seong Woon Roh
World Institute of Kimchi
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0609-6130
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Microbiome.
Background Archaea are the least-studied members of the gut-dwelling autochthonous microbiota. Few studies have reported the exclusive dominance of methanogens in the archaeal microbiome (archaeome) of the human gut, although information regarding the diversity and abundance of other archaeal phylotypes is limited.
Results We surveyed the archaeome in the faecal samples collected from 897 normal East Asian subjects living in South Korea. In total, 42.47% faecal samples were positive for archaeal colonization, which were subsequently subjected to archaeal 16S rRNA gene deep sequencing and abundance estimation. The mean archaeal abundance was 9.89 ± 4.48% of the total bacterial and archaeal abundance. We observed extensive colonization of haloarchaea (95.53%) in the Korean gut, with 9.63% mean relative abundance in archaeal communities. Haloarchaea were relatively more abundant than methanogens in certain samples. The presence of haloarchaea was also verified by fluorescence in situ hybridization analysis. Owing to large inter-individual variations, we categorized the human gut archaeome into four archaeal enterotypes.
Conclusions The study demonstrated that the human gut archaeome is indigenous, responsive, and functional, expanding our understanding of the archaeal signature in the gut of normal healthy individuals.
Keywords
Human gut, Population-level metataxonomic analysis, Archaeome, Haloarchaea, Archaeal enterotype
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This preprint is under consideration at Microbiome. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Seong Woon Roh
World Institute of Kimchi
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0609-6130
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Published
On 04 Aug, 2020
No community comments so far
Editor assigned
On 02 Jun, 2020
Submission checks complete
On 01 Jun, 2020
Editor invited
On 01 Jun, 2020
Version 1
Posted 17 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 04 May, 2020
Review #2 received
Received 28 Apr, 2020
Review #1 received
Received 24 Apr, 2020
Reviewer #2 agreed
On 28 Mar, 2020
Reviewer #1 agreed
On 26 Mar, 2020
Reviewers invited
Invitations sent on 25 Mar, 2020
Editor assigned
On 13 Mar, 2020
First submitted
On 12 Mar, 2020
Submission checks complete
On 12 Mar, 2020
Editor invited
On 12 Mar, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Microbiome.
Background Archaea are the least-studied members of the gut-dwelling autochthonous microbiota. Few studies have reported the exclusive dominance of methanogens in the archaeal microbiome (archaeome) of the human gut, although information regarding the diversity and abundance of other archaeal phylotypes is limited.
Results We surveyed the archaeome in the faecal samples collected from 897 normal East Asian subjects living in South Korea. In total, 42.47% faecal samples were positive for archaeal colonization, which were subsequently subjected to archaeal 16S rRNA gene deep sequencing and abundance estimation. The mean archaeal abundance was 9.89 ± 4.48% of the total bacterial and archaeal abundance. We observed extensive colonization of haloarchaea (95.53%) in the Korean gut, with 9.63% mean relative abundance in archaeal communities. Haloarchaea were relatively more abundant than methanogens in certain samples. The presence of haloarchaea was also verified by fluorescence in situ hybridization analysis. Owing to large inter-individual variations, we categorized the human gut archaeome into four archaeal enterotypes.
Conclusions The study demonstrated that the human gut archaeome is indigenous, responsive, and functional, expanding our understanding of the archaeal signature in the gut of normal healthy individuals.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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