The present study aims to disclose the novel activity of cellulose in nanoparticles as antioxidants, antimicrobials, antitumors and its effect on lymphocyte proliferation activity as an index of immunity. Results show that surface charge & particle size distribution of nano cellulose reveal positive zeta potentials with average values +27 mv, and size range 4.4 nm in diameter. While IC50 of antioxidants capacity was 42.1µg/mL, meanwhile antimicrobial activity impact on G-ve strains at different concentration, but not affected on tested G+ve strains and tested fungi. Also, cytotoxicity show minority for nanoparticles of cellulose. Moreover, lymphocyte proliferation assay appears highly significant effect for nano cellulose. On the other hand, antitumor power of nano cellulose on Caco-2 cancer cells was dose dependent. In addition, comet values show significant influence at dose 500 μg/mL. These findings show that cellulose play a critical role in nanoparticles when compared to conventional molecules, and that they have a remarkable physical and biological activity, indicating a promising trend for using these nano molecules in a variety of biological applications.