This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Original Article
Roghayeh Abbasalipourkabir
Hamadan University of Medical Sciences Medical School
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6345-1410
License:
This work is licensed under a CC BY 4.0 License. Read Full License
BACKGROUND: This study aimed to evaluate the effects of iron and ω-3 polyunsaturated fatty acids (PUFA) supplementations in the heart tissue of Wistar male rats with T2DM. After diabetes induced by HFD-STZ in animals, oxidative stress markers, including TOS, TAC, OSI (oxidative stress index), MDA, expression of RAGE, ICAM-1, VCAM-1, TNF-α, tissue level of triglyceride (TG), and total cholesterol in the heart of the rats receiving Ferrous sulfate and ω-3 PUFA supplements were studied. Histopathological analysis was also performed to determine cardiac injury.
RESULTS: Significant changes in the level of oxidative stress and inflammatory factors revealed the positive effect of ω-3 PUFA supplementation on diminishing the inflammatory and oxidative stress status. After comparing the group D.ω.F with the group D.ω, a significant decrease in the expression level of RAGE genes; inflammatory factors of TNF-α, ICAM-1, VCAM-1; the levels of MDA and OSI (P <0.05); and tissue level of TNF-α protein (P <0.01) was observed, indicating a negative effect of iron supplementation on ω-3 PUFA anti-inflammatory properties. In addition, a substantial increment was found in the tissue levels of total cholesterol and triglycerides in groups D and D.ω.F compared to the group CN, which indicates the effect of diabetes on increasing tissue accumulation of these two lipids. So, the consumption of these two supplements at the same time could intensify the effects of diabetes.
CONCLUSION: Ferrous sulfate supplementation neutralizes the anti-inflammatory properties of ω-3 PUFA by increasing the oxidative stress, inflammatory markers, and tissue levels of triglycerides and total cholesterol.
Keywords
Diabetes type 2, PUFAs, Iron, Oxidative stress
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Original Article
Roghayeh Abbasalipourkabir
Hamadan University of Medical Sciences Medical School
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6345-1410
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 04 Feb, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Clinical Pharmacology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
BACKGROUND: This study aimed to evaluate the effects of iron and ω-3 polyunsaturated fatty acids (PUFA) supplementations in the heart tissue of Wistar male rats with T2DM. After diabetes induced by HFD-STZ in animals, oxidative stress markers, including TOS, TAC, OSI (oxidative stress index), MDA, expression of RAGE, ICAM-1, VCAM-1, TNF-α, tissue level of triglyceride (TG), and total cholesterol in the heart of the rats receiving Ferrous sulfate and ω-3 PUFA supplements were studied. Histopathological analysis was also performed to determine cardiac injury.
RESULTS: Significant changes in the level of oxidative stress and inflammatory factors revealed the positive effect of ω-3 PUFA supplementation on diminishing the inflammatory and oxidative stress status. After comparing the group D.ω.F with the group D.ω, a significant decrease in the expression level of RAGE genes; inflammatory factors of TNF-α, ICAM-1, VCAM-1; the levels of MDA and OSI (P <0.05); and tissue level of TNF-α protein (P <0.01) was observed, indicating a negative effect of iron supplementation on ω-3 PUFA anti-inflammatory properties. In addition, a substantial increment was found in the tissue levels of total cholesterol and triglycerides in groups D and D.ω.F compared to the group CN, which indicates the effect of diabetes on increasing tissue accumulation of these two lipids. So, the consumption of these two supplements at the same time could intensify the effects of diabetes.
CONCLUSION: Ferrous sulfate supplementation neutralizes the anti-inflammatory properties of ω-3 PUFA by increasing the oxidative stress, inflammatory markers, and tissue levels of triglycerides and total cholesterol.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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