Hexachromium Cr (VI) is environmentally mobile and causes ecotoxicological hazard. Chrome utilizing industries are a major source of Cr (VI) pollution. Adsorption using activated carbon prepared from biomaterials is a new technology for the treatment of heavy metals rich industrial effluents. Opuntia Ficus Indicus (OFI) activated with H3PO4 (45%) was evaluated for Cr (VI) removal capability from synthetic and tannery wastewater. The study employed batch experiment and residual Cr (VI) concentrations were analyzed using UV-Vis spectrophotometer. FTIR spectroscopy suggested contributions of hydroxyls and carboxyls in Cr (VI) adsorption. Maximum Cr (VI) removal efficiency of 99.5% was achieved by OFI. The % removal of Cr (VI) onto OFI increased with increase of the amount of sorbent, contact time, agitating speed, while observed increased at lower values of initial concentration of Cr (VI) and PH. Optimized values of sorbent dose, adsorbate concentration, pH, contact time and stirring speed were 0.125g, 6mg/L, 2, 60minuets and 150rpm at a temperature of 250C, respectively. Langmuir isotherm with higher R2 was best fitted to the experimental values with monolayer adsorption capacity of 4.587mg/g. RL values were in range of 0 < RL < 1, confirmed the favorability of adsorption process. About 88.7% Cr (VI) removal was detected when applied to the real wastewater. The reduction in percentage of removal might be due the matrix effect of the competing ions. The study revealed; activated OFI carbon is potent biosorbent of plant origin that holds distinct position for complete removal of Cr (VI)) from aqueous solution.