Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters
Background: A bacterial biosensor refers to genetically engineered bacteria that produce an assessable signal in the presence of a physical or chemical agent in the environment.
Methods: We have designed and evaluated a bacterial biosensor expressing a luciferase-reporter gene controlled by pbr and cadA promoters in Cupriavidus metallidurans (previously termed Ralstonia metallidurans) containing the CH34 and pI258 plasmids of Staphylococcus aureus, respectively, and that can be used for the detection of heavy metals. In the present study, we have produced and evaluated biosensor plasmids designated pGL3-luc/pbr-biosensor and pGL3-luc/cad-biosensor, that were based on the expression of luc+ under the control of the cad promoter and the cadC gene of S. aureus plasmid pI258 and pbr promoter and pbrR gene from plasmid pMOL30 of Cupriavidus metallidurans.
Results: We found that the pGL3-luc/pbr-biosensor may be used to measure lead concentrations between 1-100 μM in the presence of other metals, including: zinc, cadmium, tin and nickel. The latter metals did not result in any significant signal. The pGL3-luc/cad-biosensor could detect lead concentrations between 10 nM to 10 μM.
Conclusions: This biosensor was found to be a specific for measuring lead ions in both environmental and biological samples.
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On 19 Oct, 2020
On 05 Sep, 2020
On 02 Sep, 2020
On 01 Sep, 2020
On 01 Sep, 2020
On 30 Aug, 2020
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On 28 Aug, 2020
On 01 Aug, 2020
On 29 Jul, 2020
On 28 Jul, 2020
On 28 Jul, 2020
Posted 22 Jun, 2020
On 21 Jun, 2020
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On 18 Jun, 2020
On 18 May, 2020
On 18 May, 2020
On 17 May, 2020
On 17 May, 2020
On 18 Apr, 2020
On 10 Apr, 2020
Received 09 Apr, 2020
On 07 Apr, 2020
Received 31 Mar, 2020
On 30 Mar, 2020
On 27 Mar, 2020
Invitations sent on 27 Mar, 2020
On 26 Mar, 2020
On 25 Mar, 2020
On 25 Mar, 2020
On 19 Mar, 2020
On 18 Mar, 2020
On 17 Mar, 2020
On 17 Mar, 2020
On 17 Mar, 2020
Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters
On 19 Oct, 2020
On 05 Sep, 2020
On 02 Sep, 2020
On 01 Sep, 2020
On 01 Sep, 2020
On 30 Aug, 2020
On 29 Aug, 2020
On 28 Aug, 2020
On 28 Aug, 2020
On 01 Aug, 2020
On 29 Jul, 2020
On 28 Jul, 2020
On 28 Jul, 2020
Posted 22 Jun, 2020
On 21 Jun, 2020
On 19 Jun, 2020
On 18 Jun, 2020
On 18 Jun, 2020
On 18 May, 2020
On 18 May, 2020
On 17 May, 2020
On 17 May, 2020
On 18 Apr, 2020
On 10 Apr, 2020
Received 09 Apr, 2020
On 07 Apr, 2020
Received 31 Mar, 2020
On 30 Mar, 2020
On 27 Mar, 2020
Invitations sent on 27 Mar, 2020
On 26 Mar, 2020
On 25 Mar, 2020
On 25 Mar, 2020
On 19 Mar, 2020
On 18 Mar, 2020
On 17 Mar, 2020
On 17 Mar, 2020
On 17 Mar, 2020
Background: A bacterial biosensor refers to genetically engineered bacteria that produce an assessable signal in the presence of a physical or chemical agent in the environment.
Methods: We have designed and evaluated a bacterial biosensor expressing a luciferase-reporter gene controlled by pbr and cadA promoters in Cupriavidus metallidurans (previously termed Ralstonia metallidurans) containing the CH34 and pI258 plasmids of Staphylococcus aureus, respectively, and that can be used for the detection of heavy metals. In the present study, we have produced and evaluated biosensor plasmids designated pGL3-luc/pbr-biosensor and pGL3-luc/cad-biosensor, that were based on the expression of luc+ under the control of the cad promoter and the cadC gene of S. aureus plasmid pI258 and pbr promoter and pbrR gene from plasmid pMOL30 of Cupriavidus metallidurans.
Results: We found that the pGL3-luc/pbr-biosensor may be used to measure lead concentrations between 1-100 μM in the presence of other metals, including: zinc, cadmium, tin and nickel. The latter metals did not result in any significant signal. The pGL3-luc/cad-biosensor could detect lead concentrations between 10 nM to 10 μM.
Conclusions: This biosensor was found to be a specific for measuring lead ions in both environmental and biological samples.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7