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Research article
Habib Zeighami
Zanjan University of Medical Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7467-1147
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Enterotoxigenic Bacteroides fragilis (ETBF) is an enterotoxin-producing bacterium that possibily has a role in the occurrence and progression of colorectal cancer (CRC) by modulating the mucosal immune response and inducing epithelial cell changes. The aim of this study was to investigate the frequency of ETBF in stool samples of CRC patients and healthy volunteers.
Methods
A total of 60 stool samples from confirmed CRC patients and 60 stool samples from healthy volunteers with no personal or familial history or diagnosis of colorectal disease were collected. Stool samples were screened for direct detection of B. fragilis using PCR targeting the marker genes of neu and bft. Enterotoxin isotypes bft-1, bft-2 and bft-3 were also detected in B. fragilis positive samples.
Results
The frequency of B. fragilis among CRC and control cases was 58.3% and 26.6%, respectively (P<0.05). The rate of bft gene in CRC cases was significantly higher than in controls (P<0.05). Also, the presence of bft gene in CRC patients stage III was significantly higher than stages I and II (P< 0.05). Enterotoxin isotype bft-2 was detected with higher frequency among CRC patients than healthy control (P<0.05).
Conclusion
Our results show the association between fecal ETBF and CRC, and we suggest that detection of ETBF may be a potential marker for colorectal cancer diagnosis. However, additional investigations on tumor and paired normal tissue samples are required to substantiate this possible correlation.
Keywords
Bacteroides fragilis, Enterotoxin, bft gene, Colorectal cancer, Stool
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CURRENT STATUS: Published
View the published article at BMC Cancer.
No community comments so far
Submission checks complete
On 18 Nov, 2019
Editorial decision: Accept
On 30 Aug, 2019
Editor assigned
On 29 Aug, 2019
Editor invited
On 28 Aug, 2019
No comments provided
Editorial decision: Minor revision
On 29 Aug, 2019
Editor assigned
On 15 Aug, 2019
Submission checks complete
On 14 Aug, 2019
Editor invited
On 14 Aug, 2019
Version 1
Posted 01 Jul, 2019
No comments provided
Editorial decision: Minor revision
On 13 Aug, 2019
Review #1 received
Received 01 Aug, 2019
Reviewer #1 agreed
On 22 Jul, 2019
Submission checks complete
On 28 Jun, 2019
Reviewers invited
Invitations sent on 25 Jun, 2019
Editor assigned
On 24 Jun, 2019
Editor invited
On 23 Jun, 2019
First submitted
On 31 May, 2019
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PDF Downloads: ...
HTML Views: ...
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Cancer.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Habib Zeighami
Zanjan University of Medical Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7467-1147
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Cancer.
No community comments so far
Submission checks complete
On 18 Nov, 2019
Editorial decision: Accept
On 30 Aug, 2019
Editor assigned
On 29 Aug, 2019
Editor invited
On 28 Aug, 2019
No comments provided
Editorial decision: Minor revision
On 29 Aug, 2019
Editor assigned
On 15 Aug, 2019
Submission checks complete
On 14 Aug, 2019
Editor invited
On 14 Aug, 2019
Version 1
Posted 01 Jul, 2019
No comments provided
Editorial decision: Minor revision
On 13 Aug, 2019
Review #1 received
Received 01 Aug, 2019
Reviewer #1 agreed
On 22 Jul, 2019
Submission checks complete
On 28 Jun, 2019
Reviewers invited
Invitations sent on 25 Jun, 2019
Editor assigned
On 24 Jun, 2019
Editor invited
On 23 Jun, 2019
First submitted
On 31 May, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Cancer.
Background
Enterotoxigenic Bacteroides fragilis (ETBF) is an enterotoxin-producing bacterium that possibily has a role in the occurrence and progression of colorectal cancer (CRC) by modulating the mucosal immune response and inducing epithelial cell changes. The aim of this study was to investigate the frequency of ETBF in stool samples of CRC patients and healthy volunteers.
Methods
A total of 60 stool samples from confirmed CRC patients and 60 stool samples from healthy volunteers with no personal or familial history or diagnosis of colorectal disease were collected. Stool samples were screened for direct detection of B. fragilis using PCR targeting the marker genes of neu and bft. Enterotoxin isotypes bft-1, bft-2 and bft-3 were also detected in B. fragilis positive samples.
Results
The frequency of B. fragilis among CRC and control cases was 58.3% and 26.6%, respectively (P<0.05). The rate of bft gene in CRC cases was significantly higher than in controls (P<0.05). Also, the presence of bft gene in CRC patients stage III was significantly higher than stages I and II (P< 0.05). Enterotoxin isotype bft-2 was detected with higher frequency among CRC patients than healthy control (P<0.05).
Conclusion
Our results show the association between fecal ETBF and CRC, and we suggest that detection of ETBF may be a potential marker for colorectal cancer diagnosis. However, additional investigations on tumor and paired normal tissue samples are required to substantiate this possible correlation.
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