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Komariah Komariah
Sebelas Maret University Faculty of Agriculture: Universitas Sebelas Maret Fakultas Pertanian
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7704-0754
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This work is licensed under a CC BY 4.0 License. Read Full License
Purpose: The secondary metabolites of Java tea (Orthosiphon aristatus B) are effective to treat kidney inflammation, kidney stones, and other diseases. Abiotic factors have been known to determine the secondary metabolites contents of Java tea. This study aimed at evaluating the effects of soil temperature resulted from soil mulching and harvesting period on total phenol, flavonoid and antioxidant contents of Java tea.
Methods: The research was carried out using nested with completely randomized design under a screen house at Karanganyar, Indonesia from July to December 2019. The first factor was soil mulching (control; black plastic mulch, transparent plastic mulch, biodegradable mulch and rice straw mulch) with three repetitions. The first factor was nested in second factor, namely harvesting period which consisted of two levels, i.e.: 80 and 100 days. The observation parameters were soil temperature of 10-minutes (maximum; mean; minimum and soil temperature based- growing degree days, GDD) with sensors and logger; plant growth (plant height, number of leaves, fresh and dry weight); and secondary metabolites including phenol, flavonoids and antioxidant.
Results: The results confirmed the order of the highest to the lowest mean soil temperature was resulted under the transparent plastic mulch> straw > black plastic mulch >control > biodegradable plastic mulch (26.69 > 26.29 > 26.10 > 26.07 > 25.68 oC, respectively). The total phenol, flavonoids and antioxidant produced ranging from 193.75 – 412.50 mg GAE/ 100 g DW; 81.13 – 141.47 mg QE/ 100 g DW; and 1875.5 – 2144.4 µmol TE/g DW.
Conclusion: Higher maximum soil temperature resulted into lower phenol content, while higher minimum soil temperature and shorter harvest period increased total flavonoid. Longer harvest period produced more total phenol and antioxidant due to bigger soil temperature based- Growing Degree Day (GDD).
Keywords
medicinal plants, secondary metabolite, microclimate, biodegradable plastic mulch
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Manuscript
Komariah Komariah
Sebelas Maret University Faculty of Agriculture: Universitas Sebelas Maret Fakultas Pertanian
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7704-0754
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 05 Feb, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Purpose: The secondary metabolites of Java tea (Orthosiphon aristatus B) are effective to treat kidney inflammation, kidney stones, and other diseases. Abiotic factors have been known to determine the secondary metabolites contents of Java tea. This study aimed at evaluating the effects of soil temperature resulted from soil mulching and harvesting period on total phenol, flavonoid and antioxidant contents of Java tea.
Methods: The research was carried out using nested with completely randomized design under a screen house at Karanganyar, Indonesia from July to December 2019. The first factor was soil mulching (control; black plastic mulch, transparent plastic mulch, biodegradable mulch and rice straw mulch) with three repetitions. The first factor was nested in second factor, namely harvesting period which consisted of two levels, i.e.: 80 and 100 days. The observation parameters were soil temperature of 10-minutes (maximum; mean; minimum and soil temperature based- growing degree days, GDD) with sensors and logger; plant growth (plant height, number of leaves, fresh and dry weight); and secondary metabolites including phenol, flavonoids and antioxidant.
Results: The results confirmed the order of the highest to the lowest mean soil temperature was resulted under the transparent plastic mulch> straw > black plastic mulch >control > biodegradable plastic mulch (26.69 > 26.29 > 26.10 > 26.07 > 25.68 oC, respectively). The total phenol, flavonoids and antioxidant produced ranging from 193.75 – 412.50 mg GAE/ 100 g DW; 81.13 – 141.47 mg QE/ 100 g DW; and 1875.5 – 2144.4 µmol TE/g DW.
Conclusion: Higher maximum soil temperature resulted into lower phenol content, while higher minimum soil temperature and shorter harvest period increased total flavonoid. Longer harvest period produced more total phenol and antioxidant due to bigger soil temperature based- Growing Degree Day (GDD).
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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