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Huizhen Cai
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2507-3631
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Scope The aim of this study is to examine whether lycium barbarum polysaccharide (LBP) supplementation improves hyperglycemia and inflammation in diabetic KKAy mice.
Methods The successfully established diabetic KKAy mice are randomized into five groups: diabetic model, metformin, low‐dose LBP, middle‐dose LBP, and high‐dose LBP . C57BL/6J mice are fed a chow diet as normal control . The blood glucose and body weight of mice were measured at different time points. At the end of 90 days, serum inflammatory factors were determined with ELISA kits. The expression of TLR4, MyD88, TRAF6, IκKβ, IκB, P-IκB and nuclear NF-κB proteins in mouse peritoneal macrophages were detected by Western Blotting.
Results Blood glucose decreased significantly after the intervention among low-, medium-dose LBP groups and Met group ( P <0.05). Met (40 mg/kg) inhibited the levels of IL-1β, TNF-α and IL-6 and elevated IL-10 level ( P <0.05). ELISA results showed that LBP promoted serum levels of IL-10 and decreased TNF-α level ( P <0.05). Compared with model group, KKAy mice in Met group expressed lower protein levels of MyD88, TRAF6, IκKβ, nuclear NF-κB and higher expression of IκB ( P <0.05); The expression of TLR4, MyD88, TRAF6, IκKβ and nuclear NF-κB protein in low- and medium-doses LBP groups were significantly declined ( P <0.05).
Conclusion These findings indicate that dietary supplementation with LBP can improve hyperglycemia and inflammation in diabetic KKAy mice, which can be associated with potential benefits to human health.
Keywords
Lycium barbarum polysaccharide, Type 2 diabetes mellitus, Inflammatory factor, MyD88-dependent pathway
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Huizhen Cai
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2507-3631
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
The most recent version of this article is available here.
No community comments so far
Version 1
Posted 14 Apr, 2020
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
Scope The aim of this study is to examine whether lycium barbarum polysaccharide (LBP) supplementation improves hyperglycemia and inflammation in diabetic KKAy mice.
Methods The successfully established diabetic KKAy mice are randomized into five groups: diabetic model, metformin, low‐dose LBP, middle‐dose LBP, and high‐dose LBP . C57BL/6J mice are fed a chow diet as normal control . The blood glucose and body weight of mice were measured at different time points. At the end of 90 days, serum inflammatory factors were determined with ELISA kits. The expression of TLR4, MyD88, TRAF6, IκKβ, IκB, P-IκB and nuclear NF-κB proteins in mouse peritoneal macrophages were detected by Western Blotting.
Results Blood glucose decreased significantly after the intervention among low-, medium-dose LBP groups and Met group ( P <0.05). Met (40 mg/kg) inhibited the levels of IL-1β, TNF-α and IL-6 and elevated IL-10 level ( P <0.05). ELISA results showed that LBP promoted serum levels of IL-10 and decreased TNF-α level ( P <0.05). Compared with model group, KKAy mice in Met group expressed lower protein levels of MyD88, TRAF6, IκKβ, nuclear NF-κB and higher expression of IκB ( P <0.05); The expression of TLR4, MyD88, TRAF6, IκKβ and nuclear NF-κB protein in low- and medium-doses LBP groups were significantly declined ( P <0.05).
Conclusion These findings indicate that dietary supplementation with LBP can improve hyperglycemia and inflammation in diabetic KKAy mice, which can be associated with potential benefits to human health.
Figure 1
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