ImmunoPET imaging of multiple myeloma with [68Ga]Ga-NOTA-Nb1053

Multiple myeloma (MM) remains incurable and its diagnosis relies heavily on bone marrow aspiration and biopsy. CD38 is a glycoprotein highly specific for MM. Antibody therapeutics (e.g., daratumumab) targeting CD38 have shown encouraging efficacy in treating MM, either as a monotherapy agent or in combination with other regimens. However, efficient stratification of patients who might benefit from daratumumab therapy and timely monitoring of the therapeutic responses are still clinical challenges. This work aims to devise a CD38-targeted imaging strategy and assess its value in diagnosing MMs. By labeling a CD38-specific single domain antibody (Nb1053) with 68Ga (t1/2 = 1.1 h), we developed a CD38-targeted immuno-positron emission tomography (immunoPET) imaging probe [68Ga]Ga-NOTA-Nb1053. The probe was developed with good radiochemical yield (> 50%), excellent radiochemical purity (> 99%), and immunoreactivity (> 95%). The diagnostic accuracy of the probe was thoroughly investigated in preclinical MM models. ImmunoPET imaging with [68Ga]Ga-NOTA-Nb1053 specifically depicted all the subcutaneous and orthotopic MM lesions, outperforming the traditional 18F-fluorodeoxyglucose PET and the nonspecific [68Ga]Ga-NOTA-NbGFP immunoPET. More importantly, daratumumab preloading significantly reduced [68Ga]Ga-NOTA-Nb1053 uptake in the disseminated bone lesions, indicating the overlapping targeting epitopes of [68Ga]Ga-NOTA-Nb1053 with that of daratumumab. Furthermore, premedication with sodium maleate or fructose significantly decreased kidney retention of [68Ga]Ga-NOTA-Nb1053 and improved the diagnostic value of the probe in lymphoma models. This work successfully developed a novel CD38-targeted immunoPET imaging approach that enabled precise visualization of CD38 and diagnosis of MMs. Upon clinical translation, [68Ga]Ga-NOTA-Nb1053 immunoPET may serve as a valuable CD38-targeted molecular imaging toolbox, facilitating early diagnosis of MM and precise assessment of the therapeutic responses.


Introduction
Multiple myeloma (MM) is a hematological neoplasm of B cell lineage and is characterized by uniform overexpression of CD38 [1]. Daratumumab is a monoclonal antibody (mAb) targeting CD38 and has been approved for treating patients with either newly diagnosed or relapsed/refractory MMs [2]. Several factors synergistically dictate the efficacy of daratumumab, predominantly the CD38 expression levels [3]. Besides efficiently selecting patients before the onset of CD38-targeted therapies, precisely monitoring the therapeutic responses is equally important. At the present stage, flow cytometry examination of the biopsied samples is the only way to detect the CD38 expression. However, the biopsy is invasive and limited to assess the localized myeloma lesion. Moreover, current imaging methods are less robust in detecting and quantifying minimal residual disease (MRD) [4]. To this end, there is an urgent need to develop sensitive new techniques that can noninvasively annotate CD38 expression and describe the response depth following CD38targeted therapies.
Immuno-positron emission tomography (immunoPET) is a paradigm-shifting molecular imaging technique that can detect human malignancies, inflammatory diseases, and immune cells [5][6][7]. We have developed several immunoPET probes to facilitate the early diagnosis of advanced thyroid cancer and melanoma, among others [8][9][10][11]. In the meanwhile, daratumumab has been radiolabeled for imaging CD38 in preclinical MM models [12,13], and more recently in clinical trials [14,15]. However, the size of mAb (150 kDa) exceeds the cutoff value of glomerular filtration (60 kDa) and therefore long-lived radionuclides such as 89 Zr (t 1/2 = 78.4 h) and 64 Cu (t 1/2 = 12.7 h) are needed to match the lengthy circulation. Besides, repeated imaging at several time-points is obligatory to yield optimal tumor-to-background ratio (TBR). From a clinical perspective, a feasible and reproducible imaging protocol needs to be established before carrying out mAb-based immunoPET imaging. It should not be neglected that unbound 89 Zr is a bone-seeking radiometal [9], potentially compromising the accuracy of 89 Zr-mAb in detecting disseminated MMs [14]. In this setting, molecular imaging probes with enhanced stability and smaller size would be more beneficial for clinical translation.
Owing to their smaller size (~15 kDa) and excellent antigen recognition ability, Nanobody (a trade name of Ablynx) or singl e-dom ain antibody (sdAb) has emer ged as powerful alternatives for molecular imaging [16]. Nanobody-derived immunoPET probes targeting human epidermal growth factor receptor 2 and programmed death ligand-1 have been successfully translated to the bedside [17,18]. Over the past years, we have been focusing on investigating the catalytic functions of CD38 and developing CD38-targeted therapeutics [19][20][21]. In this process, we produced a CD38-targeting sdAb (i.e., Nb1053) that shares similar binding epitopes with the daratumumab [19], indicating that Nb1053-based molecular imaging probes may select patients suitable for daratumumab treatment. In this work, we aim to develop [ 68 Ga]Ga-NOTA-Nb1053 and characterize the diagnostic value of [ 68 Ga]Ga-NOTA-Nb1053 immunoPET in preclinical MM and lymphoma models.

Cell lines and flow cytometry
A few solid cancer cell lines (i.e., A375, A549, BCPAP, TPC-1, FTC-133, and THJ-16T) and hematological neoplasm cell lines (i.e., Daudi and MM.1S) were used in the work. All the cell lines were cultured under the recommended protocols. To detect the expression of CD38 on the surface of the included cell lines, flow cytometry was carried out following the protocol we previously described [9,10]. Briefly, 1 × 10 6 cells for each sample were collected and washed with cold phosphatebuffered saline (PBS, HyClone). The washed cells were resuspended in flow cytometry staining buffer (Invitrogen) and incubated with a FITC-conjugated anti-human CD38 monoclonal antibody (Catalog#: 11-0388-42; eBioscience™) on ice for 45 min in the dark, with a concentration of 0.25 μg/mL or 0.5 μg/mL, respectively. The samples were washed again, resuspended in PBS, and analyzed with a CytoFLEX Flow Cytometer (Beckman Coulter, Inc). The results were analyzed with the FlowJo software (FlowJo LLC).

Production and characterization of radiolabeled sdAbs
Production details and sequences of Nb1053 and NbGFP are described in our previous report [19] and in the work by Kubala et al. [22]. Briefly, a llama was immunized with recombinant human CD38 (residue 45-300) and phage display technology was used to obtain the positive clones. The sequences of Nb1053, a positive clone, and NbGFP, a sdAb against the green fluorescent protein (GFP) serving as the negative control, were cloned into pET28a. The proteins with His 6 -tags were expressed in E. coli BL21 (DE3) and purified by affinity chromatography (Ni-Excel, GE Cytiva) and anionexchange chromatography (HiTrap Q, GE Cytiva). To facilitate 68 Ga-labeling, the sdAbs were first conjugated with a bifunctional chelator 2-S-(4-Isothiocyanatobenzyl)-1,4,7triazacyclononane-1,4,7-triacetic acid (p-SCN-Bn-NOTA; Macrocyclics). Briefly, 1 mg of sdAb (i.e., Nb1053 or NbGFP) in the PBS was prepared and the pH of the sdAb solution was adjusted to 9.0-10 by adding 0.1 M Na 2 CO 3 buffer. The chelator was freshly dissolved in dimethyl sulfoxide (DMSO) and immediately added to the sdAb solution with a chelator/sdAb molar ratio of 10:1. The reaction was incubated at room temperature for 2 h, followed by purification with equilibrated PD-10 desalting columns (GE Healthcare) and concentration with Amicon™ Ultra Centrifugal Filter (10 kDa, Merck). The concentrated antibody solution was stored at 4°C and was stable for at least 3 months.
For radiolabeling, 370-555 MBq of freshly eluted 68 Ga in 0.1 M hydrogen chloride (pH = 1) was mixed with equal volume of 1 M sodium acetate (pH = 5). The radiometal solution with a final volume of 2 mL (pH = 4.0-4.5) was added to 100-200 μg of NOTA-sdAb, followed by incubation of the mixture at 37°C for 5-10 min under constant shaking (600 rpm). The final radiopharmaceuticals were purified using equilibrated PD-10 columns and the radiochemical purity was assessed by instant thin-layer chromatography (iTLC; Eckert & Ziegler Radiopharma Inc.) and high-performance liquid chromatography (HPLC; Agilent). Analytical HPLC has been performed with Agilent 1260 Infinity II with a Superdex™ 75 Increase 5/ 15 GL column (5 × 153-158 mm 2 ,~9 μm). Absorbance was detected at 280 nm and radioactive signal was detected with an in-line Packard A-100 detector. To assess the immunoreactivity of [ 68 Ga]Ga-NOTA-Nb1053, 200-250 μCi of the probe in 200 μL of PBS was added to 50 μg of recombinant human CD38 protein (CD8-H5224; ACROBiosystems). The binding reaction was incubated for 15 min followed by HPLC analysis with PBS as the mobile phase at a flow rate of 0.5 mL/min.

Subcutaneous and disseminated multiple myeloma models
All animal experimental procedures and protocols had been approved by the Institutional Animal Care and Use Committee (Renji Hospital, School of Medicine, Shanghai Jiao Tong University). To establish subcutaneous and disseminated MM models, the well-recognized MM cell line MM.1S was used [23]. For subcutaneous models, 2 × 10 6 MM.1S cells were suspended in sterile PBS and matrigel matrix (Corning) with a volume ratio of 1:1 and then injected into the right posterior flanks of female NOD-Prkdc em26Cd52 Il2rg em26Cd22 / Nju mice (NCG, 4-5 weeks; GemPharmatech). For orthotopic MM models, 0.5 × 10 6 MM.1S cells in 100 μL of sterile PBS were injected via tail vein to the NCG mice. The subcutaneous and orthotopic models were ready for PET imaging 3 weeks after tumor cell inoculation. For the subcutaneous models, the average diameter of the tumor was 0.6-1.0 cm before imaging.

PET imaging and data analyses
On average, 9.05 ± 3.47 MBq (n = 16) of [ 68 Ga]Ga-NOTA-Nb1053 or 3.31 ± 0.654 MBq (n = 5) of [ 68 Ga]Ga-NOTA-NbGFP was administered to the tumor-bearing NCG mice. For mAb premedication studies, daratumumab (1 mg/mouse; Janssen Biotech, Inc.) was administered to the disseminated MM models 10 h before [ 68 Ga]Ga-NOTA-Nb1053 injection. For one specific disseminated MM model, daratumumab was administered 2 days before injection of the radiotracer. On average, 5.56 ± 0.179 MBq (n = 5) of 18 F-fluorodeoxyglucose ( 18 F-FDG) was injected via tail vein to the fasted mice, and images were acquired 1 h after tracer injection. 18 F-FDG PET imaging was performed according to a previously reported protocol with minor modification [9]. In the 1-h uptake phase, the fasted mice were warmed and kept awake to reduce brown tissue uptake and minimize unexpected deaths.
The mice were anesthetized and placed in the prone position on the scanning bed. PET/CT data were acquired in sequence with an IRIS PET/CT system (Inviscan Imaging Systems). PET data were reconstructed using a non-scattercorrected 3D-ordered subset expectation optimization/ maximum a posteriori (OSEM3D/MAP) algorithm and subsequently analyzed using the OsiriX Lite software (Pixmeo SARL) and the Inveon Research Workplace (Siemens Preclinical Solutions).

Biodistribution and histopathological studies
After termination of the immunoPET imaging studies, the mice were sacrificed and samples including blood were freshly collected and weighed. The radioactivity of the samples was counted using an automated γ-counter (Perkin-Elmer) and the uptake of the radiotracers in different organs/ tissues was calculated and given as percent of injected dose per gram of tissue (%ID/g, mean ± SD).
To rigorously evaluate the tumor burden in the MM models, both subcutaneous tumors and bone tissues (humerus, femur, and tibia) of representative NCG mice were fixed in 10% neutral buffered formalin and used for hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining. The bone tissues were decalcified in ethylene diamine tetraacetic acid and then processed in a similar way to the subcutaneous tumor samples. Sections of 10 μm were cut and stained for H&E, CD38 (Catalog#: 10818-R128, SinoBiological, and ab183326, Abcam), and CD138 (MCA2459GA, BIO-RAD) using standard protocols. To detect the location of the preinjected daratumumab (also served as the primary antibody), a horseradish peroxidase (HRP)-labeled rabbit anti-human IgG H&L (ab6759, Abcam) was used as the secondary antibody.

Statistical analysis
Statistical analysis was performed using the GraphPad software. Data are presented as means ± SD as stated in the figure legends. P < 0.05 was considered statistically significant.

Flow cytometry screening
We screened a range of cancer cell lines by flow cytometry and found that CD38 was positively expressed on two hematological malignancy cell lines (MM.1S and Daudi) (Supplemental Fig. 1). In line with the result from a previous work [25], positive expression of CD38 was also found on the A549 cell line. Besides, CD38 was negative on four thyroid cancer cell lines (BCPAP, TPC-1, FTC-133, and THJ-16T) and a melanoma cell line (A375).

Development and characterization of radiotracers
To design immunoPET imaging probes, two sdAbs targeting either GFP or human CD38 were first modified with the chelator NOTA and then radiolabeled with 68 Ga (t 1/2 = 1.1 h). The non-decay corrected radiolabeling yield of [ 68 Ga]Ga-NOTA-Nb1053 and [ 68 Ga]Ga-NOTA-NbGFP was 47.84 ± 12.2% (n = 8) and 26.43% (n = 1), respectively. The radiochemical purities of the two radiotracers were > 99% and remained >99% in the saline in 3 h (Supplemental Fig. 2). The in vivo stability of these two tracers was tested at different time points post-injection. The results showed that both the tracers were intact at 30 min and 1 h in blood and urine (data not shown). The stability of [ 68 Ga]Ga-NOTA-Nb1053 meets translational requirements because PET imaging with 68 Ga-labeled tracers usually finishes within 2 h. As shown by HPLC analysis (Fig.  1), more than 95% of [ 68 Ga]Ga-NOTA-Nb1053 complexed with its target protein CD38 and migrated rapidly than the standalone [ 68 Ga]Ga-NOTA-Nb1053 in the size exclusion chromatography study. The antibody and radioactivity retention time of [ 68 Ga]Ga-NOTA-Nb1053 were 3.506 min and 3.872 min, respectively (Fig. 1a, b). In comparison, the corresponding retention time of [ 68 Ga]Ga-NOTA-Nb1053 incubated with an excess of the extracellular domain of CD38 were 2.646 min and 2.806 min, respectively (Fig. 1c, d). These results together indicated the excellent stability and immunoreactivity of the developed [ 68 Ga]Ga-NOTA-Nb1053, warranting subsequent in vivo imaging studies.
H&E staining of a representative humerus showed diffuse bone marrow involvement by plasma cells (Fig. 5a). As illustrated by the IHC staining results (Fig. 5b), the CD138 and CD38 double-positive plasma cells diffusely replaced the normal bone marrow. We then asked if the pre-injected daratumumab dispersed into the bone marrow and bound to the plasma cells. As expected, staining of a representative humerus of the daratumumab treatment group showed bone marrow involvement by massive plasma cells (Fig. 5c). Deposition of daratumumab-bound plasma cells was found in the osteoepiphysis and diaphysis of the humerus (Fig. 5d), indicating the deposition of daratumumab in the disseminated MMs. These results together demonstrated the successful establishment of the orthotopic MM.1S models and the superior accuracy of [ 68 Ga]Ga-NOTA-Nb1053 immunoPET in delineating disseminated MMs.

Strategies reducing kidney accumulation of [ 68 Ga]Ga-NOTA-Nb1053
Although high kidney accumulation of a radioligand may not affect the diagnostic decision, it will cause unexpected nephrotoxicity when the radioligand is used for therapeutic purposes. A recent clinical trial has shown the therapeutic potential of 131 I-labeled sdAb [26]. The high kidney accumulation of [ 68 Ga]Ga-NOTA-Nb1053 largely resided in the renal cortex (Fig. 6a), which may still appear when swapping 68 Ga with therapeutic radionuclides (e.g., 177 Lu). We explored several strategies to circumvent this potential drawback at the diagnostic stage (Supplemental Table 1). It has been reported that co-injection of gelofusine and lysine could reduce kidney uptake [27]. We tested but failed to observe such an effect in our case (Supplemental Fig. 6). The osmotic diuretic mannitol also did not affect the overall and kidney uptake of [ 68 Ga]Ga-NOTA-Nb1053 (Fig. 6b). We further tested the potential role of sodium maleate and fructose in manipulating the kidney were used to stain and cross-validate the CD38 expression. ROI and biodistribution data were presented in terms of Mean ± SD uptake, both of which can decrease ATP production in the kidneys. Administration of these two compounds substantially reduced kidney uptake of [ 68 Ga]Ga-NOTA-Nb1053 (Fig.  6c, d). A higher dose of maleate (14.4 mg/mouse compared to 9.6 mg/mouse) more robustly reduced kidney accumulation of [ 68 Ga]Ga-NOTA-Nb1053 (Supplemental Fig. 7) but was associated with severe toxicity.
As revealed by the ROI analysis (Fig. 7a), the kidney uptake in the fructose intervention group and the maleate intervention group was 26.150 ± 7.948%ID/g (n = 4) and 18.725 ± 11.092%ID/g (n = 4), respectively. The uptake in the above two groups was significantly lower than that in the control group (39.100 ± 1.153%ID/g, n = 3) or the mannitol intervention group (39.467 ± 5.918%ID/g, n = 3). Moreover, sodium maleate decreased the kidney accumulation of the radiotracer more prominently than fructose (P = 0.012). These results were further consolidated by the biodistribution studies (Fig.  7b). Intraperitoneal injection of fructose slightly prolonged the circulation of [ 68 Ga]Ga-NOTA-Nb1053 and the retention of the radiotracer in several organs such as the lung, but the increase was not statistically significant compared to the other groups. These data suggest that sodium maleate is preferred when it is necessary to reduce the undesired kidney uptake of radiometal-labeled Nb1053.

Optimized [ 68 Ga]Ga-NOTA-Nb1053 immunoPET imaging of lymphomas
A previous study has indicated the potency of CD38-targeted immunoPET in imaging lymphomas [28]. Our flow cytometry results also revealed positive CD38 expression on Daudi cells. With the above information in mind, we explored the diagnostic utility of [ 68 Ga]Ga-NOTA-Nb1053 immunoPET in lymphoma xenografts in the context of maleate premedication. As shown in Supplemental Fig. 8a, [ 6 8 Ga]Ga-NOTA-Nb1053 immunoPET readily delineated subcutaneous Daudi tumors. The overall distribution and uptake features of [ 68 Ga]Ga-NOTA-Nb1053 were similar to that observed in the subcutaneous MM.1S models. Owing to the The liver, kidney, and bladder were indicated by the red arrow, yellow arrows, and white arrow, respectively. b Region of interest (ROI) analysis of the immunoPET data (n = 5). c 18 F-FDG PET imaging of subcutaneous multiple myelomas. Nonspecific uptake of 18 F-FDG in the bones (white dashed circles), muscles (red dashed circles), and brown adipose tissue compromised the specificity of 18 F-FDG in imaging the tumor (blue arrow). d Region of interest analysis of the PET data (n = 5). ROI and biodistribution data were presented in terms of Mean ± SD maleate premedication, the kidney uptake reduced substantially in the Daudi models when compared to that in the subcutaneous MM.1S models, with the value dropped from 39.460 ± 11.770%ID/g (n = 5) to 14.167 ± 4.609%ID/g (n = 3) (Supplemental Fig. 8b). The effect of maleate premedication on the kidney uptake of [ 68 Ga]Ga-NOTA-Nb1053 was further confirmed by comparing the biodistribution data of the two groups (Supplemental Fig. 8c), where the kidney uptake decreased more than 3.5 times (52.9 ± 28.9%ID/g [n = 3] vs. 187 ± 43.0%ID/g [n = 5], P = 0.0032). Although tumor uptake of [ 68 Ga]Ga-NOTA-Nb1053 was higher in the Daudi tumors than in the MM.1S tumors based on the biodistribution data (3.43 ± 1.61%ID/g [n = 3] vs. 3.02 ± 0.509%ID/g [n = 5]), the increase was not statistically significant (P = 0.5954). Finally, IHC staining further revealed intense CD38 and CD138 expression on the Daudi tumor (Supplemental Fig. 8d, e), visually more intensive than the staining intensity of the subcutaneous MM.1S tumor (Fig. 2e). These results together demonstrated the value of [ 68 Ga]Ga-NOTA-Nb1053 immunoPET in diagnosing lymphomas and the potency of maleate premedication in optimizing the TBR.

Discussion
Currently, whole-body low-dose computed tomography is preferred for detecting lytic bone lesions and magnetic resonance imaging is the method of choice for detecting bone marrow involvement by MM [29]. 18 F-FDG PET is also used to detect MRD [30,31], but its value is limited when a low hexokinase-2 expression or high background uptake occurs [32]. With the development and future clinical translation of [ 68 Ga]Ga-NOTA-Nb1053, clinicians can detect MM at earlier stages. [ 68 Ga]Ga-NOTA-Nb1053 immunoPET may detect MM-involved bones without obvious bone destruction, allowing for efficient patient stratification and selection. Additionally, this novel imaging approach can help assess treatment response and disease recurrence in a CD38dependent manner. Different from traditional mAb-based probes, the sdAb-derived [ 68 Ga]Ga-NOTA-Nb1053 will permit same-day imaging with higher TBR. This improvement eliminates the concern of nonspecific bone accumulation in the course of tedious 89 Zr-mAb immunoPET imaging [9,14]. The spleen may not capture sdAbs due to the lack of Fc fragments. Therefore, another potential merit of [ 68 Ga]Ga-NOTA-Nb1053 is that there is no need to administrate unlabeled CT images showed decreased [ 68 Ga]Ga-NOTA-Nb1053 uptake in the abovementioned bones following daratumumab premedication. The livers, kidneys, and bladders were indicated by the red arrows, yellow arrows, and white arrows, respectively Nb1053 to saturate the extremely high spleen uptake. As a companion diagnostic tool, [ 68 Ga]Ga-NOTA-Nb1053 i m m u n o P E T m a y o p t i m i z e C D 3 8 -t a r g e t e d radioimmunotherapy as well [33,34]. Considering that CD38 is an alternative tumor marker for other hematological malignancies [28], it is rational to expand the application scenarios of [ 68 Ga]Ga-NOTA-Nb1053. Indeed, [ 68 Ga]Ga-NOTA-Nb1053 immunoPET realized the precise diagnosis of lymphomas in the current work, it may also aid in the diagnosis and stratification of other solid tumors [25,35].
As seen from the data, [ 68 Ga]Ga-NOTA-Nb1053 was largely excreted from the urinary system, reabsorbed in the proximal tubule cells, and degraded by the lysosomes. The metabolic mechanism resulted in the accumulation of the radioactive catabolites in the renal cortex ( Fig. 6 and Supplemental Fig. 6). Megalin is an endocytic receptor in the proximal tubule cells and is involved in the reabsorption of proteins and peptides [36]. The plasma expander gelofusine and the positively charged basic amino acid lysine are the targets of megalin. Co-injection of gelofusine and lysine inhibited renal uptake of 99m Tc-labeled sdAb [37], but failed to reduce the kidney uptake of 68 Ga-NOTA-Nb1053 in our study. Of the several compounds tested, sodium maleate and fructose showed a robust effect in reducing kidney accumulation of [ 68 Ga]Ga-NOTA-Nb1053, presumably by decreasing ATP production in the proximal tubule cells. These observations indicate that different mechanisms are involved in the uptake of differently radiolabeled sdAbs. This knowledge may help develop new strategies to reduce renal retention of Nb1053derived radioligands and alleviate the nephrotoxicity. We are exploring other strategies (e.g., PEGylation) to increase the tumor uptake while simultaneously reducing the kidney uptake [38]. Besides, our unpublished data showed that 18 F-labeled Nb1053 shifted its excretion pathway from the urinary system to the hepatobiliary system. Such improvement is clinically relevant when radiolabeled Nb1053 is used for treatment purposes.
The treatment landscape of MM has incrementally improved in the past three decades. This success was achieved through the clinical use of proteasome inhibitors and immunomodulatory drugs, including the daratumumab. Despite the encouraging results, 40% of MM patients responded poorly to daratumumab treatment. In this setting, [ 68 Ga]Ga-NOTA-Nb1053 immunoPET may help visualize fluctuating CD38 expression, select patients, predict response, and monitor drug resistance. Since Nb1053 competes with daratumumab in binding to CD38, radiotracers binding to CD38 independently of the daratumumab (or isatuximab) will more precisely monitor the dynamic change of CD38 following the antibody therapies [39]. Although the rapid clinical translation of staining studies. a Macroscopic (left) and microscopic (black asterisk, right) H&E staining of a representative humerus. b IHC staining of the same humerus showed bone marrow involvement by CD138 and CD38 double-positive plasma cells. A monoclonal antibody (Catalog#: 10818-R128, Clone#: 128, SinoBiological) was used to stain CD38 expression and another monoclonal antibody (ab183326, Clone#: SP149, Abcam) was used to confirm the expression. c H&E staining of a representative humerus of the daratumumab treatment group (left). d For the IHC staining, the pre-loaded daratumumab served as the primary antibody. Positive staining of daratumumab was found in the osteoepiphysis (blue asterisk, middle) and diaphysis (red asterisk, right) two mAb-based CD38-targeted immunoPET probes [14,15], the development of immunoPET imaging technique with sdAbs is still in this infancy. We have demonstrated the feasibility of [ 68 Ga]Ga-NOTA-Nb1053 immunoPET in imaging MMs in preclinical models. Clinical translation of this novel CD38-targeted imaging strategy is underway. Along with the progress, we and others have developed B cell maturation antigen (BCMA)-targeting chimeric antigen receptor-  immunoPET imaging and biodistribution data. a Region of interest analysis of immunoPET imaging data. ****: P < 0.0001. *: P = 0.0120. b Ex vivo biodistribution data showing detailed uptake of [ 68 Ga]Ga-NOTA-Nb1053 in the blood, major organs, and tissues. ****: P < 0.0001. *: P = 0.0199. Data were presented in terms of Mean ± SD modified T (CAR-T) and treatment of relapsed and refractory MMs with CAR-T cell infusion resulted in a revolutionary overall response rate [40]. Therefore, alternative MM biomarkers such as BCMA and CD138 may also be leveraged in developing MM-specific molecular imaging tracers. Development and clinical translation of these state-of-the-art companion diagnostic agents will hopefully improve the clinical management of patients with MM.
To summarize, we have successfully developed [ 68 Ga]Ga-NOTA-Nb1053 and immunoPET imaging with this radiotracer visualized MM niduses with excellent clarity and accuracy.