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Bin Wu
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ORCiD: https://orcid.org/0000-0001-9078-386X
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Background: Enterococcus faecalis is an opportunistic pathogen can cause a variety of diseases, such as urinary tract infections and wound infections in human and animals. Recently, bacteriophages and their derivatives represent a good effect on fighting against bacterial infections.
Methods: We isolated virulent bacteriophages of E. faecalis using the double-layer plate method. The bio-activities of the phage isolated were determined via one-step growth curve testing and bacterial killing assays. Illumina HiSeq sequencing was performed to determine the genetic characteristics and the lysins of the phage. Protein expression and antibiofilm assays were also performed to highlight the bio-activities of the phage lysins.
Results: We isolated a virulent bacteriophage vB_EfaS_PHB08 (thereafter PHB08) from the sewage nearby hospital. PHB08 possessed a linear double-stranded DNA genome with 55,244 bp in length, which encoded 91 putative coding sequences (CDS). We found that PHB08 could inhibit the growth of host bacteria for 12 h. In vegetable models, PHB08 can reduce 1´105 Colony Forming Units (CFU) of E. faecalis per square centimeter at room temperature (25 °C) for 24 h. In addition, PHB08 and its endolysin can remove the biofilm formed by E. faecalis.
Conclusions: A virulent phage and endolysin displayed a good effect on reducing and/or eradicating E. faecalis infection and biofilm.
Keywords
Enterococcus faecalis, bacteriophage, bacterial killing, endolysin, biofilms
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Bin Wu
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9078-386X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 09 Apr, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Virology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Enterococcus faecalis is an opportunistic pathogen can cause a variety of diseases, such as urinary tract infections and wound infections in human and animals. Recently, bacteriophages and their derivatives represent a good effect on fighting against bacterial infections.
Methods: We isolated virulent bacteriophages of E. faecalis using the double-layer plate method. The bio-activities of the phage isolated were determined via one-step growth curve testing and bacterial killing assays. Illumina HiSeq sequencing was performed to determine the genetic characteristics and the lysins of the phage. Protein expression and antibiofilm assays were also performed to highlight the bio-activities of the phage lysins.
Results: We isolated a virulent bacteriophage vB_EfaS_PHB08 (thereafter PHB08) from the sewage nearby hospital. PHB08 possessed a linear double-stranded DNA genome with 55,244 bp in length, which encoded 91 putative coding sequences (CDS). We found that PHB08 could inhibit the growth of host bacteria for 12 h. In vegetable models, PHB08 can reduce 1´105 Colony Forming Units (CFU) of E. faecalis per square centimeter at room temperature (25 °C) for 24 h. In addition, PHB08 and its endolysin can remove the biofilm formed by E. faecalis.
Conclusions: A virulent phage and endolysin displayed a good effect on reducing and/or eradicating E. faecalis infection and biofilm.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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