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Research Article
Kunihiro Shiomi
Shinshu University
Corresponding Author
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The bivoltine strain of the domestic silkworm, Bombyx mori, has two generations per year. It shows a facultative diapause phenotype determined by environmental conditions, including photoperiod and temperature, and nutrient conditions during embryonic and larval development of the mother. However, it remains unclear how the environmental signals received during development are selectively utilized as cues to determine alternative diapause phenotypes. We performed a comparative analysis between the Kosetsu strain of B. mori and a Japanese population of the wild mulberry silkworm B. mandarina concerning the hierarchical molecular mechanisms in diapause induction. Our results showed that for the Kosetsu, temperature signals during the mother’s embryonic development predominantly affected diapause determination through the thermosensitive transient receptor potential ankyrin 1 (TRPA1) and diapause hormone (DH) signaling pathways. However, embryonic diapause in B. mandarina was photoperiod-dependent, although the DH signaling pathway and thermal sensitivity of TRPA1 were conserved within both species. Based on these findings, we hypothesize that TRPA1-activated signals are strongly linked to the signaling pathway participating in diapause induction in Kosetsu to selectively utilize the temperature information as the cue because temperature-dependent induction was replaced by photoperiodic induction in the TRPA1 knockout mutant.
Keywords
bivoltine strain, domestic silkworm, Bombyx mori, facultative diapause phenotype
Figure 1
Figure 2
Figure 3
Figure 4
No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.jpg
- FigS1.jpg
Supplementary Figure S1. Alignments of amino acid sequences of diapause hormone (DH) and DH receptor (DHR). The deduced amino acid sequences of DH-PBAN (a) and DHR (Acc. No. LC594680) (b) cDNAs in Bombyx mori (Bmo) and B. mandarina (Bma) were aligned. Identical amino acids are indicated by asterisks. The putative transmembrane domains (TM1~TM7) are indicated.
- FigS2.jpg
Supplementary Figure S2. Alignment of amino acid sequences of Bombyx mori and B. mandarina TRPA1. The deduced amino acid sequences of the TRPA1 cDNA (Acc. Nos. AB703646.1, LC597009.1, and LC597010.1) in B. mori (Bmo) and B. mandarina (Bma) were aligned. Identical amino acids are indicated by asterisks. The TAL repeat variable diresidues (RVDs), ankyrin repeat (1~17), linker region, transmembrane (1~6), and pore-loop domains are indicated.
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CURRENT STATUS: Published
View the published article at Scientific Reports.
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Posted 12 Feb, 2021
No community comments so far
Editorial decision: Major revision
On 02 Mar, 2021
Reviews received
Received 27 Feb, 2021
Reviewers agreed
On 17 Feb, 2021
Reviewers invited
Invitations sent on 17 Feb, 2021
Editor assigned
On 09 Feb, 2021
Editor invited
On 02 Feb, 2021
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A new preprint design is coming!
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See the published version of this preprint at Scientific Reports.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Kunihiro Shiomi
Shinshu University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Scientific Reports.
Version 1
Posted 12 Feb, 2021
No community comments so far
Editorial decision: Major revision
On 02 Mar, 2021
Reviews received
Received 27 Feb, 2021
Reviewers agreed
On 17 Feb, 2021
Reviewers invited
Invitations sent on 17 Feb, 2021
Editor assigned
On 09 Feb, 2021
Editor invited
On 02 Feb, 2021
Submission checks complete
On 02 Feb, 2021
First submitted
On 30 Jan, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Scientific Reports.
The bivoltine strain of the domestic silkworm, Bombyx mori, has two generations per year. It shows a facultative diapause phenotype determined by environmental conditions, including photoperiod and temperature, and nutrient conditions during embryonic and larval development of the mother. However, it remains unclear how the environmental signals received during development are selectively utilized as cues to determine alternative diapause phenotypes. We performed a comparative analysis between the Kosetsu strain of B. mori and a Japanese population of the wild mulberry silkworm B. mandarina concerning the hierarchical molecular mechanisms in diapause induction. Our results showed that for the Kosetsu, temperature signals during the mother’s embryonic development predominantly affected diapause determination through the thermosensitive transient receptor potential ankyrin 1 (TRPA1) and diapause hormone (DH) signaling pathways. However, embryonic diapause in B. mandarina was photoperiod-dependent, although the DH signaling pathway and thermal sensitivity of TRPA1 were conserved within both species. Based on these findings, we hypothesize that TRPA1-activated signals are strongly linked to the signaling pathway participating in diapause induction in Kosetsu to selectively utilize the temperature information as the cue because temperature-dependent induction was replaced by photoperiodic induction in the TRPA1 knockout mutant.
Figure 1
Figure 2
Figure 3
Figure 4
No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.jpg
- FigS1.jpg
Supplementary Figure S1. Alignments of amino acid sequences of diapause hormone (DH) and DH receptor (DHR). The deduced amino acid sequences of DH-PBAN (a) and DHR (Acc. No. LC594680) (b) cDNAs in Bombyx mori (Bmo) and B. mandarina (Bma) were aligned. Identical amino acids are indicated by asterisks. The putative transmembrane domains (TM1~TM7) are indicated.
- FigS2.jpg
Supplementary Figure S2. Alignment of amino acid sequences of Bombyx mori and B. mandarina TRPA1. The deduced amino acid sequences of the TRPA1 cDNA (Acc. Nos. AB703646.1, LC597009.1, and LC597010.1) in B. mori (Bmo) and B. mandarina (Bma) were aligned. Identical amino acids are indicated by asterisks. The TAL repeat variable diresidues (RVDs), ankyrin repeat (1~17), linker region, transmembrane (1~6), and pore-loop domains are indicated.
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