1 Kaur S, Dhillon GS. Recent trends in biological extraction of chitin from marine shell wastes, A review. Crit Rev Biotechnol. 2015, 35:44-61.
2 Yan N, Chen X. Don't waste seafood waste. Nature 2015, 524:155-157.
3 Wei G, Zhang A, Chen K, Ouyang P. Enzymatic production of N-acetyl-D-glucosamine from crayfish shell wastes pretreated via high pressure homogenization. Carbohyd Polym. 2017, 171:236-241.
4 Duan B, Huang Y, Lu A, Zhang LN. Recent advances in chitin based materials constructed via physical methods. Prog Polym Sci. 2018, 82:1-33.
5 Miguel SP, Moreira AF, Correia IJ. Chitosan based-asymmetric membranes for wound healing: A review. Int J Biol Macromol. 2019, 127:460-475.
6 Shanmuganathan R, Edison TNJI, LewisOscar F, Kumar P, Shanmugam S, Pugazhendhi A. Chitosan nanopolymers: An overview of drug delivery against cancer. Int J Biol Macromol. 2019, 130:727-736.
7 Chen JK, Shen CR, Liu CL. N-acetylglucosamine: Production and applications. Mar Drugs. 2010, 8:2493-2516.
8 Jung W-J, Park R-D. Bioproduction of chitooligosaccharides: present and perspectives. Mar Drugs. 2014, 12:5328-5356.
9 Bezouska K, Sklenar J, Dvorakova J , Havlicek V, Pospisil M, Thiem J, Kren, V. Nkr-p1a protein, an activating receptor of rat natural killer cells, binds to the chitobiose core of uncompletely glycosylated N-linked glycans, and to linear chitooligomers. Biochem Bioph Res Co. 1997, 238:149-153.
10 Samain E, Drouillard S, Heyraud A, Driguez H, Geremia RA. Gram-scale synthesis of recombinant chitooligosaccharides in Escherichia coli. Carbohyd Res. 1997, 302:35-42.
11 Suzuki K, Mikami T, Okawa Y, Tokoro A, Suzuki S, Suzuki M. Antitumor effect of hexa-N-acetyl-chitohexose and chitohexose. Carbohyd Res. 1986, 151:403-408.
12 Roby D, Gadelle A. Chitin oligosaccharides as elicitors of chitinase activity in melon plants. Biochem Bioph Res Co. 1987, 143:885-892.
13 Gao C, Zhang A, Chen K, Hao Z, Tong J, Ouyang P: Characterization of extracellular chitinase from Chitinibacter sp GC72 and its application in GlcNAc production from crayfish shell enzymatic degradation. Biochem Eng J. 2015, 97:59-64.
14 Zhang A, Wei GG, Mo XF, Zhou N, Chen KQ, Ouyang PK. Enzymatic hydrolysis of chitin pretreated by bacterial fermentation to obtain pure N-acetyl-D-glucosamine. Green Chem. 2018, 20:2320-2327.
15 Guo XX, Xu P, Zong MH, Lou WY. Purification and characterization of alkaline chitinase from Paenibacillus pasadenensis CS 0611. Chin J Catal. 2017, 38:665-672.
16 Adrangi S, Faramarzi MA. From bacteria to human: A journey into the world of chitinases. Biotechnol Adv. 2013, 31:1786-1795.
17 Yang SQ, Song S, Yan QJ, Fu X, Jiang ZQ, Yang XB. Biochemical characterization of the first fungal glycoside hydrolyase family 3 beta-N-acetylglucosaminidase from Rhizomucor miehei. J Agr Food Chem. 2014, 62:5181-5190.
18 Vaaje-Kolstad G, Westereng B, Horn SJ, Liu ZL, Zhai H, Sorlie M, Eijsink VGH. An oxidative enzyme boosting the enzymatic conversion of recalcitrant polysaccharides. Science 2010, 330:219-222.
19 Tsujibo H, Kondo N, Tanaka K, Miyamoto K, Baba N, Inamori Y. Molecular analysis of the gene encoding a novel transglycosylative enzyme from Alteromonas sp strain O-7 and its physiological role in the chitinolytic system. J Bacteriol. 1999, 181:5461-5466.
20 Nyffenegger C, Nordvang RT, Zeuner B, Lezyk M, Difilippo E, Logtenberg MJ, Schols HA, Meyer AS, Mikkelsen JD. Backbone structures in human milk oligosaccharides: Trans-glycosylation by metagenomic beta-N-acetylhexosaminidases. Appl Microbiol Biotechnol. 2015, 99:7997-8009.
21 Visnapuu T, Teze D, Kjeldsen C, Lie A, Duus JO, Andre-Miral C, Pedersen LH, Stougaard P, Svensson B. Identification and characterization of a beta-N-acetylhexosaminidase with a biosynthetic activity from the marine bacterium Paraglaciecola hydrolytica S66(t). Int J Mol Sci. 2020, 21.
22 Zhang R, Zhou J, Song Z, Huang Z. Enzymatic properties of β-N-acetylglucosaminidases. Appl Microbiol Biotechnol. 2018, 102: 93–103.
23 Chen X, Xu L, Jin L, Sun B, Gu G, Lu L, Xiao M. Efficient and regioselective synthesis of beta-GalNAc/GlcNAc-lactose by a bifunctional transglycosylating beta-N-acetylhexosaminidase from Bifidobacterium bifidum. Appl Environ Microbiol. 2016, 82:5642-5652.
24 Muschiol J, Vuillemin M, Meyer AS, Zeuner B. β-N-acetylhexosaminidases for carbohydrate synthesis via Trans-Glycosylation. Catalysts. 2020, 10:365.
25 Fu X, Yan Q, Yang S, Yang X, Guo Y, Jiang Z. An acidic, thermostable exochitinase with beta-N-acetylglucosaminidase activity from Paenibacillus barengoltzii converting chitin to N-acetyl glucosamine. Biotechnol Biofuels. 2014,7.
26 Lemieux MJ, Mark BL, Cherney MM, Withers SG, Mahuran DJ, James MN. Crystallographic structure of human beta-hexosaminidase a: Interpretation of tay-sachs mutations and loss of gm2 ganglioside hydrolysis. J Mol Biol. 2006, 359:913-929.
27 Zhou JP, Song ZF, Zhang R, Chen CH, Wu Q, Li JJ, Tang XH, Xu B, Ding JM, Han NY, Huang ZX. A shinella beta-N-acetylglucosaminidase of glycoside hydrolase family 20 displays novel biochemical and molecular characteristics. Extremophiles 2017, 21:699-709.
28 Singh S, Gallagher R, Derrick PJ, Crout DHG: Glycosidase-catalyzed oligosaccharide synthesis-preparation of the N-acetylchitooligosaccharides penta-N-acetylchitopentaose and hexa-N-acetylchitohexaose using the beta-N-acetylhexosaminidase of Aspergillus-oryzae. Tetrahedron-Asymmetry. 1995, 6:2803-2810.
29 Singh S, Packwood J, Samuel CJ, Critchley P, Crout DHG. Glycosidase-catalysed oligosaccharide synthesis: Preparation of N-acetylchitooligosaccharides using the beta-N-acetylhexosaminidase of Aspergillus oryzae. Carbohyd Res. 1995, 279:293-305.
30 Rauvolfová J, Weignerová L, Kuzma M, Přikrylová V, Macková M, Pišvejcová A, Křen Vr. Enzymatic synthesis of N-acetylglucosaminobioses by reverse hydrolysis: Characterisation and application of the library of fungal β-N-acetylhexosaminidases. J Mol Catalysis B: Enzymatic. 2004, 29:259-264.
31 Rajnochova E, Dvorakova J, Hun kova Z, Kren V. Reverse hydrolysis catalysed by β-N-acetylhexosaminidase from Aspergillus oryzae. Biotechnol Lett. 1997, 19:869-872.
32 Bojarova P, Slamova K, Krenek K, Gazak R, Kulik N, Ettrich R, Pelantova H, Kuzma M, Riva S, Adamek D, Bezouska K, Kren V. Charged hexosaminides as new substrates for beta- N- acetylhexosaminidasecatalyzed synthesis of immunomodulatory disaccharides. Adv Synth Catal. 2014, 356:259-259.
33 Nieder V, Kutzer M, Kren V, Gallego RG, Kamerling JP, Elling LJE. Screening and characterization of β-N-acetylhexosaminidases for the synthesis of nucleotide-activated disaccharides. Enzyme Microb Tech. 2004, 34:407-414.
34 Dvorakova J, Schmidt D, Hunkova Z, Thiem J, Kren V. Enzymatic rearrangement of chitine hydrolysates with beta-N-acetylhexosaminidase from Aspergillus oryzae. J Mol Catalysis B: Enzymatic. 2001, 11:225-232.
35 Tsujibo H, Kondo N, Tanaka K, Miyamoto K, Baba N, Inamori Y. Molecular analysis of the gene encoding a novel transglycosylative enzyme from Alteromonas sp. Strain O-7 and its physiological role in the chitinolytic system. J Bacteriol. 1999, 181:5461-5466.
36 Kurakake M, Goto T, Ashiki K, Suenaga Y, Komaki T. Synthesis of new glycosides by transglycosylation of N-acetylhexosaminidase from Serratia marcescens YS-1. J Agr Food Chem. 2003, 51:1701.
37 Takahashi M, Mashiyama T, Suzuki T: Purification and some characteristics of beta-N-acetylglucosaminidase produced by Vibrio sp. Journal of Fermentation and Bioengineering. 1993, 76:356-360.
38 Hao ZK, Cai YJ, Liao XR, Liang XH, Liu JY, Fang ZY, Hu MM, Zhang DB. Chitinolyticbacter meiyuanensis SYBC-H1(t), gen. Nov., sp nov., a chitin-degrading bacterium isolated from soil. Curr Microbiol. 2011, 62:1732-1738.
39 Perkins DN, Pappin DJC, Creasy DM, Cottrell JS. Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis. 1999, 20:3551-3567.
40 Huang L, Garbulewska E, Sato K, Kato Y, Nogawa M, Taguchi G, Shimosaka M. Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase. J Biosci Bioeng. 2012, 113:293-299.
41 Ueda M, Fujita Y, Kawaguchi T, Arai M. Cloning, nucleotide sequence and expression of the beta-N-acetylglucosaminidase gene from Aeromonas sp no. 10S-24. J Biosci Bioeng. 200, 89:164-169.
42 Tews I, Perrakis A, Oppenheim A, Dauter Z, Wilson KS, Vorgias CE. Bacterial chitobiase structure provides insight into catalytic mechanism and the basis of tay-sachs disease. Nature StrucT Bio. 1996, 3:638-648.
43 Lin H, Xiao X, Zeng X, Wang FP: Expression, characterization and mutagenesis of the gene encoding beta-N-acetylglucosaminidase from Aeromonas caviae CB101. Enzyme Microb Tech. 2006, 38:765-771.
44 Suginta W, Chuenark D, Mizuhara M, Fukamizo T. Novel beta-N-acetylglucosaminidases from Vibrio harveyi 650: Cloning, expression, enzymatic properties, and subsite identification. Bmc Biochem. 2010, 11:12.
45 Matsuo Y, Kurita M, Park JK, Tanaka K, Nakagawa T, Kawamukai M, Matsuda H. Purification, characterization and gene analysis of N-acetylglucosaminidase from Enterobacter sp G-1. Biosci Biotechnol Biochem. 1999, 63:1261-1268.
46 Lan XQ, Ozawa N, Nishiwaki N, Kodaira R, Okazaki M, Shimosaka M. Purification, cloning, and sequence analysis of beta-N-acetylglucosaminidase from the chitinolytic bacterium Aeromonas hydrophila strain SUWA-9. Biosci Biotechnol Biochem. 2004;68:1082-1090.
47 Lonhienne T, Zoidakis J, Vorgias CE, Feller G, Gerday C, Bouriotis V. Modular structure, local flexibility and cold-activity of a novel chitobiase from a Psychrophilic antarctic bacterium. J Mol Bio. 2001, 310:291-297.
48 Prag G, Papanikolau Y, Tavlas G, Vorgias CE, Petratos K, Oppenheim AB. Structures of chitobiase mutants complexed with the substrate di-N-acetyl-d-glucosamine: The catalytic role of the conserved acidic pair, aspartate 539 and glutamate 540. J Mol Bio. 2000, 300:611-617.
49 Zhang R, Zhou J, Song Z, Huang Z. Enzymatic properties of beta-N-acetylglucosaminidases. Appl Microbiol Biotechnol. 2018,102:93-103.
50 Sumida T, Ishii R, Yanagisawa T, Yokoyama S, Ito M. Molecular cloning and crystal structural analysis of a novel beta-N-acetylhexosaminidase from Paenibacillus sp TS12 capable of degrading glycosphingolipids. J Mol Bio. 2009, 392:87-99.
51 Herlihey FA, Moynihan PJ, Clarke AJ.The essential protein for bacterial Flagella formation FLGJ functions as a beta-N-acetylglucosaminidase. J Biol Chem. 2014;289:31029-31042.
52 Mayer C, Vocadlo DJ, Mah M, Rupitz K, Stoll D, Warren RAJ, Withers SG. Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi. Febs J. 2006, 273:2929-2941.
53 Chen F, Chen X-Z, Qin L-N, Tao Y, Dong Z-Y. Characterization and homologous overexpression of an N-acetylglucosaminidase nag1 from Trichoderma reesei. Biochem Bioph Re Co. 2015, 459:184-188.
54 Li H, Morimoto K, Katagiri N, Kimura T, Sakka K, Lun S, Ohmiya K. A novel beta-N-acetylglucosaminidase of Clostridium paraputrificum M-21 with high activity on chitobiose. Appl Microb Biotechnol. 2002, 60:420-427.
55 Tews Vvo RV, Vorgias CE. N-acetylglucosaminidase (chitobiase) from Serratia marcescens: Gene sequence, and protein production and purification in Escherichia coli. Gene (Amsterdam) 1996;170:63-67.
56 Senba M, Kashige N, Miake F, Watanabe K: Purification and properties of three beta-n-acetylglucosaminidases from Lactobacillus casei ATCC 27092. Biosci Biotechnol Biochem. 1998, 62:404-406.
57 Zhou J, Song Z, Zhang R, Ding L, Wu Q, Li J, Tang X, Xu B, Ding J, Han N, Huang Z: Characterization of a NaCl-tolerant beta-N-acetylglucosaminidase from Sphingobacterium sp hwlb1. Extremophiles. 2016, 20:547-557.
58 Tsujibo H, Miyamoto K, Yoshimura M, Takata M, Miyamoto J, Inamori Y: Molecular cloning of the gene encoding a novel beta-N-acetylhexosaminidase from a marine bacterium, Alteromonas sp strain O-7, and characterization of the cloned enzyme. Biosci Biotechnol Biochem. 2002, 66:471-475.
59 Ogawa M, Kitagawa M, Tanaka H, Ueda K, Watsuji T, Beppu T, Kondo A, Kawachi R, Oku T, Nishio T: A beta-n-acetylhexosaminidase from symbiobacterium thermophilum; gene cloning, overexpression, purification and characterization. Enzyme Microb Technol. 2006, 38:457-464.
60 Konno N, Takahashi H, Nakajima M, Takeda T, Sakamoto Y: Characterization of beta-n-acetylhexosaminidase (lehex20a), a member of glycoside hydrolase family 20, from lentinula edodes (shiitake mushroom). Amb Express 2012;2
61 Suginta W, Chuenark D, Mizuhara M, Fukamizo T. Novel beta-N-acetylglucosaminidases from Vibrio harveyi 650: Cloning, expression, enzymatic properties, and subsite identification. Bmc Biochem. 2010,11.
62 Krolicka M, Hinz SWA, Koetsier MJ, Eggink G, van den Broek LAM, Boeriu CG: Beta-N-acetylglucosaminidase MthNAG from Myceliophthora thermophila C1, a thermostable enzyme for production of N-acetylglucosamine from chitin. Appl Microb Biotechnol. 2018, 102:7441-7454.
63 Stleger RJ, Cooper RM, Charnley AK: Characterization of chitinase and chitobiase produced by the entomopathogenic fungus Metarhizium-anisopliae. J Invertebrate Pathology. 1991, 58:415-426.
64 Bhuvanachandra B, Podile AR: A transglycosylating chitinase from Chitiniphilus shinanonensis (CsChil) hydrolyzes chitin in a processive manner. Int J Biol Macromol. 2020, 145:1-10.
65 Usui T, Matsui H, Isobe K. Enzymic-synthesis of useful chito-oligosaccharides utilizing transglycosylation by chitinolytic enzymes in a buffer containing ammonium-sulfate. Carbohydrate Res.1990;203:65-77.
66 Vaikuntapu PR, Mallakuntla MK, Das SN, Bhuvanachandra B, Ramakrishna B, Nadendla SR, Podile AR. Applicability of endochitinase of Flavobacterium johnsoniae with transglycosylation activity in generating long-chain chitooligosaccharides. Int J Biol Macromol. 2018, 117:62-71.
67 Lee HJ, Lee YS, Choi YL. Cloning, purification, and characterization of an organic solvent-tolerant chitinase, MtCh509, from Microbulbifer thermotolerans DAU221. Biotechnol Biofuels. 2018, 11:14.
68 Kurakake M, Goto T, Ashiki K, Suenaga Y, Komaki T: Synthesis of new glycosides by transglycosylation of N-acetylhexosaminidase from Serratia marcescens YS-1. J Agr Food Chem. 2003, 51:1701.
69 Fumio, Nanjo, Mariko, Ishikawa, Ryosuke, Katsumi, Kazuo, Agricultural SJ, Chemistry B: Purification, properties, and transglycosylation reaction of β-N-acetylhexosaminidase from Nocardia orientalis. Agricultural & Biological Chemistry. 2014, 54:1990
70 Zhang AL, Gao C, Wang J, Chen KQ, Ouyang PK. An efficient enzymatic production of N-acetyl-D-glucosamine from crude chitin powders. Green Chem. 2016, 18:2147-2154.
71 Laemmli UK: Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature. 1970, 227:680-685.
72 Gasteiger E, Gattiker A, Hoogland C, Ivanyi I, Appel RD, Bairoch A. The proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res. 2003, 31:3784-3788.
73 Letunic I, Doerks T, Bork P. Smart: Recent updates, new developments and status in 2015. Nucleic Acids Res. 2015, 43:257-260.
74 Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K, Madden TL: Blast plus : Architecture and applications. Bmc Bioinformatics. 2009, 10.
75 Armenteros JJA, Tsirigos KD, Sonderby CK, Petersen TN, Winther O, Brunak S, von Heijne G, Nielsen H. Signalp 5.0 improves signal peptide predictions using deep neural networks. Nature Biotechnol. 2019, 37:420.
76 Shen H-B, Chou K-C. Gneg-mploc: A top-down strategy to enhance the quality of predicting subcellular localization of gram-negative bacterial proteins. J Theor Biol. 2010, 264:326-333.
77 Robert X, Gouet P. Deciphering key features in protein structures with the new endscript server. Nucleic Acids Res. 2014, 42:W320-W324.
78 Letunic I, Bork P. 20 years of the smart protein domain annotation resource. Nucleic Acids Res. 2018, 46:493-496.
79 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976, 72:248-254.
80 Zhang AL, He YM, Wei GG, Zhou J, Dong WL, Chen KQ, Ouyang PK. Molecular characterization of a novel chitinase CmChi1 from Chitinolyticbacter meiyuanensis SYBC-H1 and its use in N-acetyl-D-glucosamine production. Biotechnol Biofuels. 2018, 11:14.
81 Price NC: The determination of km values from lineweaver-burk plots. Biochem Education. 1985, 13:81-81.