See the published version of this preprint at BMC Ophthalmology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Research article
Wang liya
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2164-0512
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background Fungal Keratitis (FK) is an infective keratopathy with extremely high blindness rate. The damaging effect of this disease is not only the destruction of corneal tissue during fungal infection, but also the cornea scar formed during the healing period after infection control, which can also seriously affect a patient's vision. The purpose of the study was to observe the effect of umbilical cord mesenchymal stem cells (uMSCs) on corneal scar formation in FK. Methods The FK mouse model was made according to a previously reported method. Natamycin eye drops were used for antifungal treatment 24 hours after modeling. There are four groups involved in the study, including control group, FK group, vehicleinj FK group and uMSCsinj FK group. Mice in uMSCsinj FK group received repeated subconjunctival injections of uMSCs for 3 times at the 1d, 4d and 7d after FK modeling. At 14d, 21d and 28d after trauma, clinical observation, histological examination, second harmonic generation and molecular assays were performed. Results The uMSCs topical administration reduced corneal scar formation area and corneal opacity, accompanying with decreased corneal thickness and inflammatory cell infiltration, following down-regulated fibrotic-related factors α-SMA, TGFβ1, CTGF, and COLI and finally inhibited phosphorylation of TGFβ1/Smad2 signaling pathway during FK corneal fibrosis. Conclusion The results confirmed that uMSCs can improve corneal opacity during the scar formation stage of FK, and exert anti-inflammatory and anti-fibrotic effects.
Keywords
Fungal keratitis, corneal fibrosis, umbilical cord mesenchymal stem cells
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Ophthalmology.
No community comments so far
Editorial decision: Minor revision
On 25 Oct, 2019
Review #1 received
Received 23 Aug, 2019
Editor assigned
On 31 Jul, 2019
Reviewers invited
Invitations sent on 31 Jul, 2019
Reviewer #1 agreed
On 31 Jul, 2019
Submission checks complete
On 30 Jul, 2019
Editor invited
On 30 Jul, 2019
Version 2
Posted 25 Jun, 2019
No comments provided
Editorial decision: Major revision
On 15 Jul, 2019
Review #2 received
Received 09 Jul, 2019
Review #1 received
Received 27 Jun, 2019
Reviewer #1 agreed
On 25 Jun, 2019
Reviewer #2 agreed
On 25 Jun, 2019
Reviewers invited
Invitations sent on 25 Jun, 2019
Submission checks complete
On 20 Jun, 2019
Editor invited
On 20 Jun, 2019
Editor assigned
On 20 Jun, 2019
No comments provided
Editorial decision: Major revision
On 30 May, 2019
Review #2 received
Received 22 May, 2019
Reviewer #2 agreed
On 13 May, 2019
Review #1 received
Received 14 Mar, 2019
Reviewers invited
Invitations sent on 18 Feb, 2019
Reviewer #1 agreed
On 18 Feb, 2019
Submission checks complete
On 08 Jan, 2019
Editor invited
On 08 Jan, 2019
Editor assigned
On 04 Jan, 2019
First submitted
On 27 Dec, 2018
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Internal Medicine Specialties
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Ophthalmology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Wang liya
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2164-0512
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Ophthalmology.
No community comments so far
Editorial decision: Minor revision
On 25 Oct, 2019
Review #1 received
Received 23 Aug, 2019
Editor assigned
On 31 Jul, 2019
Reviewers invited
Invitations sent on 31 Jul, 2019
Reviewer #1 agreed
On 31 Jul, 2019
Submission checks complete
On 30 Jul, 2019
Editor invited
On 30 Jul, 2019
Version 2
Posted 25 Jun, 2019
No comments provided
Editorial decision: Major revision
On 15 Jul, 2019
Review #2 received
Received 09 Jul, 2019
Review #1 received
Received 27 Jun, 2019
Reviewer #1 agreed
On 25 Jun, 2019
Reviewer #2 agreed
On 25 Jun, 2019
Reviewers invited
Invitations sent on 25 Jun, 2019
Submission checks complete
On 20 Jun, 2019
Editor invited
On 20 Jun, 2019
Editor assigned
On 20 Jun, 2019
No comments provided
Editorial decision: Major revision
On 30 May, 2019
Review #2 received
Received 22 May, 2019
Reviewer #2 agreed
On 13 May, 2019
Review #1 received
Received 14 Mar, 2019
Reviewers invited
Invitations sent on 18 Feb, 2019
Reviewer #1 agreed
On 18 Feb, 2019
Submission checks complete
On 08 Jan, 2019
Editor invited
On 08 Jan, 2019
Editor assigned
On 04 Jan, 2019
First submitted
On 27 Dec, 2018
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Internal Medicine Specialties
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Ophthalmology.
Background Fungal Keratitis (FK) is an infective keratopathy with extremely high blindness rate. The damaging effect of this disease is not only the destruction of corneal tissue during fungal infection, but also the cornea scar formed during the healing period after infection control, which can also seriously affect a patient's vision. The purpose of the study was to observe the effect of umbilical cord mesenchymal stem cells (uMSCs) on corneal scar formation in FK. Methods The FK mouse model was made according to a previously reported method. Natamycin eye drops were used for antifungal treatment 24 hours after modeling. There are four groups involved in the study, including control group, FK group, vehicleinj FK group and uMSCsinj FK group. Mice in uMSCsinj FK group received repeated subconjunctival injections of uMSCs for 3 times at the 1d, 4d and 7d after FK modeling. At 14d, 21d and 28d after trauma, clinical observation, histological examination, second harmonic generation and molecular assays were performed. Results The uMSCs topical administration reduced corneal scar formation area and corneal opacity, accompanying with decreased corneal thickness and inflammatory cell infiltration, following down-regulated fibrotic-related factors α-SMA, TGFβ1, CTGF, and COLI and finally inhibited phosphorylation of TGFβ1/Smad2 signaling pathway during FK corneal fibrosis. Conclusion The results confirmed that uMSCs can improve corneal opacity during the scar formation stage of FK, and exert anti-inflammatory and anti-fibrotic effects.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
Loading...