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Research article
Baolong Xing
Shanxi Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1360-5338
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Mung bean (Vigna radiata) is a warm-season legume crop and belongs to papilionoid subfamily of the Fabaceae. China is the leading producer of mung bean in the world. It has significant economic and health benefits and is a promising species with broad adaptation and high tolerance to stress environments. The OSCA family members play an important role in the modulation of hypertonic stresses, such as drought and salinity. However, genome-wide analysis of the OSCA family in mung bean is lacking.
Results
We identified a total of 13 OSCA genes in the mung bean genome and named according to their homology with AtOSCAs. All the OACAs were phylogenetically splitted into four clades. Phylogenetic relationship and synteny analyses showed that the VrOSCAs in mung bean and soybean shared a relatively conserved evolutionary history. In addition, three duplicated VrOSCA gene pairs were identified and the duplicated VrOSCA shave mainly undergone purifying selection pressure during evolution. Protein domain, motif and transmembrane analysis indicated that most of the VrOSCAs shared similar structures with their homologs. The expression pattern showed that exception of VrOSCA2.1, the other 12VrOSCAs were up-regulated expression under treatment with ABA, PEG and NaCl, among which VrOSCA1.4 showed the largest increased expression levels. The duplicated genes VrOSCA2.1/VrOSCA2.2 showed divergence expression, which might experience functionalization during subsequent evolution. The expression profiles under ABA, PEG and NaCl stress revealed a functional divergence of VrOSCA genes, which agreed with the cis-acting elements analysis in the promoter of VrOSCA genes.
Conclusions
Collectively, the study provided a systematic analysis of the VrOSCA family in mung bean. Our results would lay an important foundation for functional and evolutionary analysis of VrOSCAs, and provide promising genes for further investigation of abiotic stress tolerance in mung bean.
Keywords
Mung bean (Vigna radiata), OSCA gene family, Evolutionary analysis, Expression patterns, Abiotic stresses
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.xlsx
The OSCAs amino acid sequences in mung bean, soybean, Arabidopsis and rice.
- Additionalfile2.xlsx
Synteny analysis of OSCA genes between mung bean and other plant species.
- Additionalfile3.xlsx
The position of the transmembrane region of VrOSCAs.
- Additionalfile4.xlsx
Sequences of the 20 motifs in the VrOSCAs.
- Additionalfile5.xlsx
Positions of abiotic stress-responsive cis-acting elements in the 1.5 kb upstream promoter of VrOSCA genes.
- Additionalfile6.xlsx
Expression patterns of VrOSCA genes in response to ABA, PEG and NaCl treatments.
- Additionalfile7.xlsx
PCR primers used for qRT-PCR in this study.
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CURRENT STATUS: Under Review
Version 1
Posted 01 Feb, 2021
No community comments so far
Review #2 received
Received 07 Mar, 2021
Editorial decision: Major revision
On 07 Mar, 2021
Review #3 received
Received 03 Mar, 2021
Review #1 received
Received 15 Feb, 2021
Reviewer #3 agreed
On 10 Feb, 2021
Reviewer #2 agreed
On 06 Feb, 2021
Reviewer #1 agreed
On 04 Feb, 2021
Editor assigned
On 30 Jan, 2021
Reviewers invited
Invitations sent on 30 Jan, 2021
Submission checks complete
On 30 Jan, 2021
Editor invited
On 24 Jan, 2021
First submitted
On 18 Jan, 2021
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Baolong Xing
Shanxi Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1360-5338
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 01 Feb, 2021
No community comments so far
Review #2 received
Received 07 Mar, 2021
Editorial decision: Major revision
On 07 Mar, 2021
Review #3 received
Received 03 Mar, 2021
Review #1 received
Received 15 Feb, 2021
Reviewer #3 agreed
On 10 Feb, 2021
Reviewer #2 agreed
On 06 Feb, 2021
Reviewer #1 agreed
On 04 Feb, 2021
Editor assigned
On 30 Jan, 2021
Reviewers invited
Invitations sent on 30 Jan, 2021
Submission checks complete
On 30 Jan, 2021
Editor invited
On 24 Jan, 2021
First submitted
On 18 Jan, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Mung bean (Vigna radiata) is a warm-season legume crop and belongs to papilionoid subfamily of the Fabaceae. China is the leading producer of mung bean in the world. It has significant economic and health benefits and is a promising species with broad adaptation and high tolerance to stress environments. The OSCA family members play an important role in the modulation of hypertonic stresses, such as drought and salinity. However, genome-wide analysis of the OSCA family in mung bean is lacking.
Results
We identified a total of 13 OSCA genes in the mung bean genome and named according to their homology with AtOSCAs. All the OACAs were phylogenetically splitted into four clades. Phylogenetic relationship and synteny analyses showed that the VrOSCAs in mung bean and soybean shared a relatively conserved evolutionary history. In addition, three duplicated VrOSCA gene pairs were identified and the duplicated VrOSCA shave mainly undergone purifying selection pressure during evolution. Protein domain, motif and transmembrane analysis indicated that most of the VrOSCAs shared similar structures with their homologs. The expression pattern showed that exception of VrOSCA2.1, the other 12VrOSCAs were up-regulated expression under treatment with ABA, PEG and NaCl, among which VrOSCA1.4 showed the largest increased expression levels. The duplicated genes VrOSCA2.1/VrOSCA2.2 showed divergence expression, which might experience functionalization during subsequent evolution. The expression profiles under ABA, PEG and NaCl stress revealed a functional divergence of VrOSCA genes, which agreed with the cis-acting elements analysis in the promoter of VrOSCA genes.
Conclusions
Collectively, the study provided a systematic analysis of the VrOSCA family in mung bean. Our results would lay an important foundation for functional and evolutionary analysis of VrOSCAs, and provide promising genes for further investigation of abiotic stress tolerance in mung bean.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.xlsx
The OSCAs amino acid sequences in mung bean, soybean, Arabidopsis and rice.
- Additionalfile2.xlsx
Synteny analysis of OSCA genes between mung bean and other plant species.
- Additionalfile3.xlsx
The position of the transmembrane region of VrOSCAs.
- Additionalfile4.xlsx
Sequences of the 20 motifs in the VrOSCAs.
- Additionalfile5.xlsx
Positions of abiotic stress-responsive cis-acting elements in the 1.5 kb upstream promoter of VrOSCA genes.
- Additionalfile6.xlsx
Expression patterns of VrOSCA genes in response to ABA, PEG and NaCl treatments.
- Additionalfile7.xlsx
PCR primers used for qRT-PCR in this study.
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