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Research Article
Hongbo Qi
Chongqing Medical University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3776-0487
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective
To investigate the effect of IL-27 on Th1 cells infiltration in human fetal membranes (FMs) and the underlying mechanisms in preterm labor.
Methods
The expression of IL-27 receptor a subunit (IL-27R𝛼) and Th1 cells were studied in human FMs from pregnant women with preterm labor (PL) and term labor (TL). In vitro, we investigated the effects of IL-27 in human amniotic cell lineage on the Th1 related chemokines and adhesive molecules and the underlying intracellular signaling molecules.
Results
The expression of IL-27R𝛼 was higher in human fetal membranes from PL group compared with TL group. Compared with TL group, the PL group had more Th1 cells infiltration in human FMs. Meanwhile, it was confirmed the expression of CXCL9, CXCL10, CXCL11 and ICAM-1 were higher in FMs from PL group to TL group. Moreover, IL-27 stimulation can up-regulate the expression of those chemokines in human amniotic cell lineage via JAK2/STAT1/STAT3 signaling pathway.
Conclusions
The expression of IL-27R𝛼 and number of Th1 cells infiltration were higher at FMs of PL group than TL group. IL-27 could promote Th1 related chemokines and adhesive molecules in human amniotic cell lineage mainly through JAK2/STAT1/STAT3 signaling pathway.
Keywords
IL-27, Preterm Labor, Th1 cells, maternal-fetal interface
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A new preprint design is coming!
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This preprint is under consideration at Inflammation. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Hongbo Qi
Chongqing Medical University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3776-0487
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 09 Mar, 2021
No community comments so far
Reviewers invited
Invitations sent on 01 Mar, 2021
First submitted
On 15 Jan, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective
To investigate the effect of IL-27 on Th1 cells infiltration in human fetal membranes (FMs) and the underlying mechanisms in preterm labor.
Methods
The expression of IL-27 receptor a subunit (IL-27R𝛼) and Th1 cells were studied in human FMs from pregnant women with preterm labor (PL) and term labor (TL). In vitro, we investigated the effects of IL-27 in human amniotic cell lineage on the Th1 related chemokines and adhesive molecules and the underlying intracellular signaling molecules.
Results
The expression of IL-27R𝛼 was higher in human fetal membranes from PL group compared with TL group. Compared with TL group, the PL group had more Th1 cells infiltration in human FMs. Meanwhile, it was confirmed the expression of CXCL9, CXCL10, CXCL11 and ICAM-1 were higher in FMs from PL group to TL group. Moreover, IL-27 stimulation can up-regulate the expression of those chemokines in human amniotic cell lineage via JAK2/STAT1/STAT3 signaling pathway.
Conclusions
The expression of IL-27R𝛼 and number of Th1 cells infiltration were higher at FMs of PL group than TL group. IL-27 could promote Th1 related chemokines and adhesive molecules in human amniotic cell lineage mainly through JAK2/STAT1/STAT3 signaling pathway.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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