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Research article
Takashige KASHIMOTO
Kitasato Univ.
Corresponding Author
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Background: Vibrio vulnificus hemolysin (VVH) is a pore-forming toxin secreted by Vibrio vulnificus . Cellular cholesterol was believed to be the receptor for VVH, because cholesterol could bind to VVH and preincubation with cholesterol inhibited cytotoxicity. It has been reported that specific glycans such as N-acetyl-D-galactosamine and N-acetyl-D-lactosamine bind to VVH, however, it has not been known whether these glycans could inhibit the cytotoxicity of VVH without oligomer formation. Thus, to date, binding mechanisms of VVH to cellular membrane, including specific receptors have not been elucidated. Results: We show here that VVH associates with ganglioside GM1a, Fucosyl-GM1, GD1a, GT1c, and GD1b by glycan array. Among them, GM1a could pulldown VVH. Moreover, the GD1a inhibited the cytotoxicity of VVH without the formation of oligomers. Conclusion: This is the first report of a molecule able to inhibit the binding of VVH to target cells without oligomerization of VVH.
Keywords
Gangliosides, Hemolysin, Receptor, Vibrio
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CURRENT STATUS: Published
View the published article at BMC Microbiology.
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Posted 24 Mar, 2020
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On 20 Mar, 2020
Editorial decision: Accept
On 19 Mar, 2020
No comments provided
Submission checks complete
On 13 Mar, 2020
Editorial decision: Minor revision
On 13 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 10 Mar, 2020
Review #3 received
Received 06 Mar, 2020
Reviewer #3 agreed
On 20 Feb, 2020
Reviewer #2 agreed
On 17 Feb, 2020
Review #2 received
Received 17 Feb, 2020
Reviewers invited
Invitations sent on 14 Feb, 2020
Reviewer #1 agreed
On 14 Feb, 2020
Review #1 received
Received 14 Feb, 2020
Editor assigned
On 12 Feb, 2020
Submission checks complete
On 11 Feb, 2020
Editor invited
On 11 Feb, 2020
No comments provided
Editorial decision: Major revision
On 04 Dec, 2019
Review #3 received
Received 26 Nov, 2019
Review #2 received
Received 25 Nov, 2019
Reviewer #3 agreed
On 18 Nov, 2019
Reviewer #2 agreed
On 14 Nov, 2019
Review #1 received
Received 28 Oct, 2019
Reviewer #1 agreed
On 18 Oct, 2019
Reviewers invited
Invitations sent on 14 Jul, 2019
Submission checks complete
On 01 Jul, 2019
Editor assigned
On 24 Jun, 2019
Editor invited
On 23 Jun, 2019
First submitted
On 16 Jun, 2019
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Subject Areas
General Microbiology
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Microbiology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Takashige KASHIMOTO
Kitasato Univ.
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Microbiology.
Version 4
Posted 24 Mar, 2020
No community comments so far
Submission checks complete
On 20 Mar, 2020
Editorial decision: Accept
On 19 Mar, 2020
No comments provided
Submission checks complete
On 13 Mar, 2020
Editorial decision: Minor revision
On 13 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 10 Mar, 2020
Review #3 received
Received 06 Mar, 2020
Reviewer #3 agreed
On 20 Feb, 2020
Reviewer #2 agreed
On 17 Feb, 2020
Review #2 received
Received 17 Feb, 2020
Reviewers invited
Invitations sent on 14 Feb, 2020
Reviewer #1 agreed
On 14 Feb, 2020
Review #1 received
Received 14 Feb, 2020
Editor assigned
On 12 Feb, 2020
Submission checks complete
On 11 Feb, 2020
Editor invited
On 11 Feb, 2020
No comments provided
Editorial decision: Major revision
On 04 Dec, 2019
Review #3 received
Received 26 Nov, 2019
Review #2 received
Received 25 Nov, 2019
Reviewer #3 agreed
On 18 Nov, 2019
Reviewer #2 agreed
On 14 Nov, 2019
Review #1 received
Received 28 Oct, 2019
Reviewer #1 agreed
On 18 Oct, 2019
Reviewers invited
Invitations sent on 14 Jul, 2019
Submission checks complete
On 01 Jul, 2019
Editor assigned
On 24 Jun, 2019
Editor invited
On 23 Jun, 2019
First submitted
On 16 Jun, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Microbiology.
Background: Vibrio vulnificus hemolysin (VVH) is a pore-forming toxin secreted by Vibrio vulnificus . Cellular cholesterol was believed to be the receptor for VVH, because cholesterol could bind to VVH and preincubation with cholesterol inhibited cytotoxicity. It has been reported that specific glycans such as N-acetyl-D-galactosamine and N-acetyl-D-lactosamine bind to VVH, however, it has not been known whether these glycans could inhibit the cytotoxicity of VVH without oligomer formation. Thus, to date, binding mechanisms of VVH to cellular membrane, including specific receptors have not been elucidated. Results: We show here that VVH associates with ganglioside GM1a, Fucosyl-GM1, GD1a, GT1c, and GD1b by glycan array. Among them, GM1a could pulldown VVH. Moreover, the GD1a inhibited the cytotoxicity of VVH without the formation of oligomers. Conclusion: This is the first report of a molecule able to inhibit the binding of VVH to target cells without oligomerization of VVH.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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