Investıgatıon of The Dıagnostıc and Prognostıc Values of Some Specıfıc mıcroRNAs In Menıngıom Tumors

doi:10.21203/rs.3.rs-1944804/v1

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Investıgatıon of The Dıagnostıc and Prognostıc Values of Some Specıfıc mıcroRNAs In Menıngıom Tumors

https://doi.org/10.21203/rs.3.rs-1944804/v1

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Objective

Primary brain tumors are classified as glial or non-glial and benign or malignant. Menenjiomas are common benign intracranial tumors. Although the name meningioma refers to a tumor of the lining of the brain called the 'Meninx', it has actually been shown to originate from the spider web-shaped 'arachnoid' membrane (arachnoid cover cells). MicroRNAs are 18–22 nucleotide long, endogenous, non-protein-coding RNA molecules that negatively regulate gene expression at the post-transcriptional level. In this study, we applied a genome-wide array screen comparing the expression of miR-145, miR-34a-3p, miR-200a, miR-335, miR-106a-5p, miR-219-5p, miR-375, miR-409-3p miR-197 and miR-224 in meningiomas.

Patients and methods

A total of 40 meningioma patients (13 men, 27 women) and healthy control individuals (12 men, 18 women) aged between 30 and 65 were inclusives in the study. The research was conducted at Gazi University Hospital.

Results

In our study, miR-197 identified as the most highly expressed miRNA in meningiomas compared to other miRNAs. miR-197, miR-34a, miR-375, miR-219 a and miR-224 stand out as potential biomarkers in human serum samples of meningiomas patients. Moreover, as per WHO classification miR-197, miR-34a, miR-375 might be used as potentail biomarkes for grade I meningioma while miR-375 for grade II meningioma.

Conclusion

The role of miRNAs in meningiomas is gaining importance each day. Therefore, our study examining the role of miRNAs in meningiomas will shed more light and pave the way for future therapeutic strategy.

Biomarker

Meningioma

miRNA

miR-197

miR-34a-3p

Meningiomas are diffuse central nervous system (CNS) tumors that account for approximately one-third of all intracranial tumors[1; 2]. According to the World Health Organization (WHO) classification, the vast majority of menenjiomas are grade 1 (typical/benign), less than 10% grade II (atypical / moderate) and III (anaplastic / malignant) tumors[3]. Their incidence increases with age with a female : male ratio of 2 : 1. Furthermore, findings from autopsy and imaging studies show that the prevalence of subclinical meningiomas constitutes roughly 3% of the population[4]. It has been implicated in the pathogenesis of many tumors, such as inactivation of tumor suppressor genes or overexpression of oncogenes and specific gene dysfunctions[5]. The most common abnormality identified in meningiomas is deletions in chromosome 22. Loss of chromosomes 1p, 3p, 6q, 10q and 14q were also observed, especially in atypical or malignant meningiomas. It has already been suggested that these deletions can be used as a marker to predict tumor progression[6]. MicroRNAs are 18–22 nucleotide long, endogenous, non-protein-coding RNA molecules that negatively regulate gene expression at the post-transcriptional level[7]. In recent years, there has been an increasing research on miRNAs as potential biomarkers for various pathological conditions, including tumors[8]. MiRNAs can function as oncogenes or tumor suppressors under certain conditions. There are evidences that they play roles in many cellular processes that contribute to tumor formation and development, from proliferation to invasion, from metastasis to angiogenesis[8; 9]. Many studies analyzing the profile and function of miRNA have yielded important insights into the pathogenesis of different types of tumors, including glioblastoma multiforme (GBM), breast cancer, pancreatic cancer, lung cancer, prostate cancer, and colon cancer[10; 11; 12; 13; 14]. The recent findings indicating that miRNAs are expressed in human blood holds great promise that circulating miRNAs can serve as novel molecular biomarkers for cancer[14; 15]. In present study, the association between meningioma and gene expression of miR-145, miR-34a-3p, miR-200a, miR-335, miR-106a-5p, miR-219-5p, miR-375, miR-409-3p miR-197 and miR-224 were examined in tumor samples obtained from patients with meningioma and healthy individual samples as controls.

Study populations

A total of 40 patients who applied to the Department of Neurosurgery and diagnosed with meningioma from brain tumors and 30 healthy volunteers were included in the study. Healthy group were selected from those who applied to gazi hospital's check-up center and whose blood came to the central laboratory, and those who met our admission and exclusion criteria. Some of the serums obtained from the routine blood of these people were reserved for the study. Likewise, some of the serums obtained from the blood samples taken from meningioma patients at the time of diagnosis were separated. All separated serums were stored at -80 degrees until analysis.

For the qRT-PCR assay, a fixed constant of synthetic cel-miR-39 (Genepharm, China) was added to 100 µl serum sample as the internal control. Total RNA isolation from samples was performed using the QIAGEN miRNeasy Serum/Plasma Advanced (Cat.no:217204) kit. Overall, total RNA was obtained from 200µl serum in a yield of approximately 75–100µng. The cDNA synthesis was made from the RNA samples using the kit (Cat.No:339340, miRCURY LNA RT Kit, Qiagen). qRT-PCR assay was performed by BioMark HD system (Fluidigm, South San Francisco, CA, USA) according to the manufacturer’s instructions. All reactions, was performed in triplicate, including controls without template RNA, The expression levels of selected miRNAs were normalized to cel-miR-39[15].

Statistical analyses

For the statistical evaluation, the conformity of the variables to the normal distribution was examined with the Shapiro-Wilk test. Normally distributed t-test was used to compare the groups. The data which were not normally distributed, Mann-Whitney U test for comparison analyzes between two independent groups, Kruskal Wallis test for comparison analyzes between three groups [post hoc test: Mann-Whitney U test with Bonferroni correction descriptive statistics are expressed as Mean ± SE and Mean ± SD. One-Way Anova:Post-hoc:Tukey HSD Test, tamhane t2 test, Dunnett test, Kruskal Wallis H test, Post hoc:Dunn Sidak tests were used to compare the expression levels of miRNAs. Statistical significance was accepted as p < 0.05. IBM SPSS package program version 22 was used in the evaluation of the data.

A total of 40 patients who applied to the Department of Neurosurgery and diagnosed with meningioma from brain tumors and 30 healthy volunteers were included in the study. Having 40 meningioma patients with a mean age of 48.77 ± 13.09 and a healthy control with a mean age of 30 44.34 ± 15.04 years. A total of 70 participants were included in this study [Table 1].

A multiphase study was designed to identify markedly altered miRNAs in serum that could distinguish meningioma from healthy controls and serve as potential biomarkers for meningioma. The study was conduceted to compare the expression of human miRNAs in pooled serum samples from 40 preoperative meningioma participants with those from 30 healthy controls for initial screening. The serum fold change of the preoperative group compared with the healthy control group is shown in Fig. 1.

The expression levels of the 10 miRNAs in the 40 pre-operative serum samples were stratified using 3 types of clinicopathological parameters (age, sex and WHO tumor grad. When classified according to tumor grade, miR-197-5p, miR-34a-3p and miR-375 showed significant differences associated with pathological grades (Fig. 2).

ROC curve analyzes were performed to evaluate the diagnostic accuracy of miRNAs in the serum between meningioma patients and normal subjects. The AUCs and 95% confidence intervals [CI] for miR-145, miR-34a-3p, miR-200a, miR-335, miR-106a-5p, miR-219-5p, miR-375, miR-409-3p miR-197 and miR-224 is shown in Fig. 3

Table 1

Age and gender data of groups
	Age (Mean ± SD)	Sex
	Age (Mean ± SD)	Male (Mean ± SD)	Female (Mean ± SD)
Meningiom	48,77 ± 13, 9	46,92 ± 10, 9 (n:13)	49, 81 ± 11, 4 (n:27)
Healty	44,34 ± 15, 4	47,72 ± 12, 7 (n:12)	40, 16 ± 16, 7 (n:18)
P value	p > 0,05	p > 0,05	p > 0,05

MicroRNAs play important roles in post-transcriptional gene regulation. It is known to regulate many pathways involved in cell proliferation, morphogenesis and differentiation[9]. Saydam et al examined the relationship between different types of miRNAs and meningiomas and found that it altered miRNA levels in different types of meningiomas, with increased expression in some and decreased expression in others. They found an increase in the expression levels of miR-335, miR-98 and miR-181a, and a decrease in the expression levels of miRNA-200a, miRNA-373 and miRNA-575 in different meningioma cases. Moreover, miRNA-200a inhibited meningioma growth during in vitro studies[16]. Zhi et al. Various miRNAs (including miR-106a-5p, miR-219 − 5p, miR-375, miR-197, miR-224 and miR-409 − 3p) are known to be dysregulated in meningioma patients[17].

In previous studies, downregulation of miR-34a-3p in high-grade meningiomas has been reported[18; 19]. miR-34a-3p targets the Bcl-2 gene in HBL-52 cells. It has been suggested that high expression of Bcl-2 may contribute to increased cell proliferation and decreased apoptosis of tumor cells[20]. Overexpression of miR-34a-3p in meningioma inhibits cell proliferation and induces apoptosis. Therefore, it is thought that miR-34a-3p can be used as an anticancer drug in meningiomas[21]. In our study, similar results were obtained which complements previous studies on the miR-34a-3p expression in meningiomas. We found that miR-34a-3p expression was lower in the meningioma patient group compared to the healthy control group (p < 0.001).

miR-145 expression was seen in atypical and anaplastic tumours as crosschecked to benign meningiomas[22]. Moreover, meningioma cells with high miR-145 levels had impaired migratory and invasive potential in vitro and in vivo. PCR-array studies of miR145- overexpressing cells suggested that collagen type V alpha (COL5A1) expression was down regulated as a consequence of miR-145 over expression. Accordingly, COL5A1 expression was significantly upregulated in atypical and anaplastic meningiomas [23].

miR-197 has been extensively studied in the carcinogenesis progression of cancers through a variety of mechanisms, including apoptosis, proliferation, angiogenesis, metastasis, drug resistance and tumor suppressor, and has also been implicated in the prognosis of cancers[24; 25; 26]. El-Gewely et al. found that miR-197 was lowly expressed in meningioma cells, while IGFBP5 was highly expressed[19]. They demonstrated that miR-197 inhibits the pro-apoptotic IGFBP5 gene by binding to conserved binding sites in its 3′UTR[19]. In a study conducted by Zhi et al[27]. in meningiomas, it was found that miR-197 expression was lower than in healthy controls. In our study, meningioma patients showed higher expression of miR-197 than controls (p = 0.003) which is contrary to the study of Zhi et al[17]. The expression of miR-197 in our study may differ from previous studies for reasons such as sample size, study method, gender, race and environmental factors.

miR-375-5p is highly expressed in meningiomas[17]. therewithal, it is thought that miR-375-5p may be an important biomarker in meningioma patients pre- and post-operatively[17]. In our study, miR-375 expression was found to be lower in meningioma patients compared to controls (p = 0.003). We think that our results would be an important milestone for future studies, since no studies of miR-375 related to meningioma have been found in the literature till date.

There is evidence that miR-219-5p, which can individuate meningioma patients from healthy group with high sensitivity, can discriminate pre- and post-operatively from meningioma patients, can help monitor the effect of surgical resection in clinical practice[27]. The widespread use of this miRNA panel is believed to have significant potential as aa conbined diagnostic and monitoring biomarker for meningioma[27; 28]. Similar results were obtained for the miR-219a-5p in our study. It was observed that miR-219a-5p was expressed more in the meningioma patient group than in the healthy control group (p = 0.0012).

In a study investigating miR-224 expression and its relationship with histological grading and postoperative recurrence in patients with meningioma, it was found that IOMM-Lee and CH157 inhibited miR-224 expression in meningioma cells, and that miR-224 inhibition increased apoptosis and suppressed cell proliferation[29]. In addition, this study identified ERG2 as a new target of miR-224 and indicated that the miR-224-ERG2 axis played a critical role in regulating the apoptosis and proliferation of meningioma cells[29]. Considering the grades, miR-224 in grade I was found to be lower and therefore it is thought to be an important biomarker when separated by grades [17]. Wang et al.[29] studied miRNA-224 in meningioma and found significantly higher miRNA-224 expression in meningioma and a positive correlation between tumor grade and miRNA-224 expression in tissues as well as compared to normal tissues was found. Patients with low miRNA-224 expression had a significantly longer disease course and lower recurrence rate compared to other cases of meningiomas. In our study, the expression of meningioma patiens was found to be lower than healthy controls (p = 0.03) indicating that our results are consistent with the literature and expression of miR-224 has been found to be lower in grade I.

In summary, we tried to demonstrate that miRNAs can be an important potential biomarker target for meningiomas in our present study. In particular, miR-197 was expressed more than other miRNAs in this group of patients. Therefore, it may be a potential biomarker target in the pre-operative process. miR-197, miR-34a, miR-375, miR-219a and miR-224 stand out as potential biomarkers in meningiomas in human serum samples. It also strengthens the possibility that miR-197, miR-34a, miR-375 in grade I and miR-375 in grade II could be used as a potential biomarker in our meningioma.

There is no known biomarker for the diagnosis and evaluation of the prognosis of meningioma disease yet. In the last decade, it has been shown that epigenetic factors play important roles in tumor pathogenesis. In particular, non-coding RNA molecules such as miRNAs may be potential new therapeutic targets in meningiomas. In addition, thanks to the potential use of miRNAs, tumor diagnosis can be made easily in the early period non-invasively, and pre-surgical planning can be made in terms of prognosis, and some of the intraoperative difficulties (waiting for biopsy results, etc.) will be eliminated. In our study, miR-197, miR-219a expressions were higher in meningioma patients compared to controls, and miR-34a, miR-224 and miR-375 expressions were lower in controls. We observed that the expressions of miR-335, miR-409, miR-106 and miR-145 did not show any significant difference. However, miR-197 was found to be the most highly expressed miRNA in meningiomas compared to other miRNAs. miR-197, miR-34a, miR-375, miR-219a and miR-224 stand out as potential biomarker targets in human serum samples of meningiomas patients. In addition, according to the WHO classification, miR-197, miR-34a, miR-375 grade I and miR-375 grade II also strengthened the possibilities that they could be used as a potential biomarker for meningiomas. However, there is a need for further studies on these miRNAs with more comprehensive inclusion of patients.

Acknowledgements The authors would like to thank Gazi University Scientific Research Council for financial support (Project No: TDK-2021-6994) and the present study is a part from the Phd thesis entitled: Investıgatıon of The Dıagnostıc And Prognostıc Values of Some Specıfıc mıcroRNAs In Menıngıom Tumors.

Author contributions All authors contributed to the study conception and design. All authors read and approved the final manuscript.

Data availability Data are available upon reasonable request.

Conflict of interest There is no conflict of interest among the authors.

Ethics approval The study was approved by the Clinical Research Ethics Committee of Gazi University, Ankara, Turkey.

Consent to participate Informed consent was obtained from all individual participants included in the study.

There is no conflict of interest among the authors.

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Investıgatıon of The Dıagnostıc and Prognostıc Values of Some Specıfıc mıcroRNAs In Menıngıom Tumors

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Abstract

Objective

Patients and methods

Results

Conclusion

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Introduction

Materials And Methods

Results

Discussion

Conclusion

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References

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Version 1