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Research
Libao Ma
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8454-6996
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Background
Intestinal surface epithelial cells (IECs) have long been considered an effective barrier for maintaining water and electrolyte balance and participating in the absorption of nutrients. When intestinal inflammation occurs, IECs tend to malfunction. Berberine (BBR) is an isoquinoline alkaloid found as the major alkaloid in many medicinal plants, which has been clinically used in China to treat gastrointestinal pathogenic bacterial infection, especially bacteria-induced diarrhea and inflammation.
Methods
We treated rat intestinal epithelial cells IEC-18 with lipopolysaccharide to establish an in vitro model of epithelial cell inflammation and used berberine to treat the cells in order to explore the anti-inflammatory mechanism of berberine. We then used transcriptome data to find the differentially expressed genes (DEGs) in each group, and analyzed DEGs by GO, KEGG, WGCNA and IPATH to find the functions and pathways enriched by DEGs. Finally, we used q-pcr to vertify our transcriptome dates.
Results
We found DEGs between LPS and LPS+BBR groups are enriched in DNA replication, cell cycle, apoptosis, leukocyte migration, NF-κB and Ap-1pathway. The results showed berberine can restrict DNA replication, inhibits cell cycle and promote apoptosis. It can also inhibit the traditional inflammatory pathways such as NF-κB, Ap-1 and the expression of various chemokines to prevent the migration of leukocyte.
Conclusion
According to our transcriptomics dates, berberine can exert anti-inflammatory effect by regulating a variety of cellular physiological activities like cell cycle, apoptosis, inflammation pathways and leukocyte migration.
Keywords
transcriptomics, intestinal inflammation, cell cycle, apoptosis, NF-κB pathway, leukocyte migration
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Libao Ma
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8454-6996
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 08 Apr, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Intestinal surface epithelial cells (IECs) have long been considered an effective barrier for maintaining water and electrolyte balance and participating in the absorption of nutrients. When intestinal inflammation occurs, IECs tend to malfunction. Berberine (BBR) is an isoquinoline alkaloid found as the major alkaloid in many medicinal plants, which has been clinically used in China to treat gastrointestinal pathogenic bacterial infection, especially bacteria-induced diarrhea and inflammation.
Methods
We treated rat intestinal epithelial cells IEC-18 with lipopolysaccharide to establish an in vitro model of epithelial cell inflammation and used berberine to treat the cells in order to explore the anti-inflammatory mechanism of berberine. We then used transcriptome data to find the differentially expressed genes (DEGs) in each group, and analyzed DEGs by GO, KEGG, WGCNA and IPATH to find the functions and pathways enriched by DEGs. Finally, we used q-pcr to vertify our transcriptome dates.
Results
We found DEGs between LPS and LPS+BBR groups are enriched in DNA replication, cell cycle, apoptosis, leukocyte migration, NF-κB and Ap-1pathway. The results showed berberine can restrict DNA replication, inhibits cell cycle and promote apoptosis. It can also inhibit the traditional inflammatory pathways such as NF-κB, Ap-1 and the expression of various chemokines to prevent the migration of leukocyte.
Conclusion
According to our transcriptomics dates, berberine can exert anti-inflammatory effect by regulating a variety of cellular physiological activities like cell cycle, apoptosis, inflammation pathways and leukocyte migration.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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