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Research article
Xu-Fang Liang
Corresponding Author
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Background: As economical traits, food habits domestication can reduce production cost in aquaculture. However, the molecular mechanism underlying food habits domestication has remained elusive. Mandarin fish (Siniperca chuatsi) only feed on live prey fish and refuse artificial diets. In the present study, we domesticated mandarin fish to feed on artificial diets. The two groups were obtained, the fish did not eat artificial diets or ate artificial diets during all of the three domestication processes, named Group W or X, respectively.
Results: Using transcriptome and metabolome analysis, we investigated the differentially expressed genes and metabolites between the two groups, and found three common pathways related to food habit domestication, including retinol metabolism, glycerolipid metabolism, and biosynthesis of unsaturated fatty acids pathways. Furthermore, the western blotting and bisulfite sequencing PCR analysis were performed. The gene expression of TFIIF and histone methyltransferase ezh1 were significantly increased and decreased in the fish of Group X, respectively. The total DNA methylation levels of TFIIF gene and tri-methylation of histone H3 at lysine 27 (H3K27me3) were significantly higher and lower in the fish of Group X, respectively.
Conclusion: It was speculated that mandarin fish which could feed on artificial diets, might be attributed to the lower expression of ezh1, resulting in the decreased level of H3K27me3 and increased level of DNA methylation of TFIIF gene. The high expression of TFIIF gene might up-regulate the expression of genes in retinol metabolism, glycerolipid metabolism and glycerophosphoric metabolism pathways. Our study indicated the relationship between the methylation of DNA and histone and food habits domestication, which might be a novel molecular mechanism of food habits domestication in animals.
Keywords
Mandarin fish, Transcriptome sequencing, Metabolome, H3K27 tri-methylation, DNA methylation, Food habits domestication
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View the published article at BMC Genomics.
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Submission checks complete
On 15 Jan, 2021
Editorial decision: Minor revision
On 12 Jan, 2021
Version 2
Posted 04 Dec, 2020
No comments provided
Editorial decision: Minor revision
On 22 Dec, 2020
Review #1 received
Received 12 Dec, 2020
Reviewer #1 agreed
On 22 Nov, 2020
Editor assigned
On 21 Nov, 2020
Reviewers invited
Invitations sent on 21 Nov, 2020
Submission checks complete
On 21 Nov, 2020
Editor invited
On 21 Nov, 2020
No comments provided
Editorial decision: Major revision
On 26 Sep, 2020
Review #2 received
Received 23 Sep, 2020
Reviewer #2 agreed
On 03 Sep, 2020
Review #1 received
Received 22 Jul, 2020
Reviewer #1 agreed
On 15 Jul, 2020
Reviewers invited
Invitations sent on 02 Jul, 2020
Editor assigned
On 24 Jun, 2020
Submission checks complete
On 16 Jun, 2020
Editor invited
On 15 Jun, 2020
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Subject Areas
Epigenetics & Genomics
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A new preprint design is coming!
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See the published version of this preprint at BMC Genomics.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Xu-Fang Liang
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Genomics.
No community comments so far
Submission checks complete
On 15 Jan, 2021
Editorial decision: Minor revision
On 12 Jan, 2021
Version 2
Posted 04 Dec, 2020
No comments provided
Editorial decision: Minor revision
On 22 Dec, 2020
Review #1 received
Received 12 Dec, 2020
Reviewer #1 agreed
On 22 Nov, 2020
Editor assigned
On 21 Nov, 2020
Reviewers invited
Invitations sent on 21 Nov, 2020
Submission checks complete
On 21 Nov, 2020
Editor invited
On 21 Nov, 2020
No comments provided
Editorial decision: Major revision
On 26 Sep, 2020
Review #2 received
Received 23 Sep, 2020
Reviewer #2 agreed
On 03 Sep, 2020
Review #1 received
Received 22 Jul, 2020
Reviewer #1 agreed
On 15 Jul, 2020
Reviewers invited
Invitations sent on 02 Jul, 2020
Editor assigned
On 24 Jun, 2020
Submission checks complete
On 16 Jun, 2020
Editor invited
On 15 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Genomics.
Background: As economical traits, food habits domestication can reduce production cost in aquaculture. However, the molecular mechanism underlying food habits domestication has remained elusive. Mandarin fish (Siniperca chuatsi) only feed on live prey fish and refuse artificial diets. In the present study, we domesticated mandarin fish to feed on artificial diets. The two groups were obtained, the fish did not eat artificial diets or ate artificial diets during all of the three domestication processes, named Group W or X, respectively.
Results: Using transcriptome and metabolome analysis, we investigated the differentially expressed genes and metabolites between the two groups, and found three common pathways related to food habit domestication, including retinol metabolism, glycerolipid metabolism, and biosynthesis of unsaturated fatty acids pathways. Furthermore, the western blotting and bisulfite sequencing PCR analysis were performed. The gene expression of TFIIF and histone methyltransferase ezh1 were significantly increased and decreased in the fish of Group X, respectively. The total DNA methylation levels of TFIIF gene and tri-methylation of histone H3 at lysine 27 (H3K27me3) were significantly higher and lower in the fish of Group X, respectively.
Conclusion: It was speculated that mandarin fish which could feed on artificial diets, might be attributed to the lower expression of ezh1, resulting in the decreased level of H3K27me3 and increased level of DNA methylation of TFIIF gene. The high expression of TFIIF gene might up-regulate the expression of genes in retinol metabolism, glycerolipid metabolism and glycerophosphoric metabolism pathways. Our study indicated the relationship between the methylation of DNA and histone and food habits domestication, which might be a novel molecular mechanism of food habits domestication in animals.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
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