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Background: The small intestine, while serving as the main absorption organ, also possesses a unique microbiome environment and holds the critical function of conversion of primary bile acids. Bile acids are, in turn, able to regulate microbiome structure and promote the growth of bacteria that convert primary bile acids to secondary bile acids. However, in beef cattle, few studies have explored the microbiome compositions of the jejunum and its relationships with bile acids. Therefore, the hypothesis is that microbiome compositions in the intestine are influenced by diet style and also related to bile acids.
Results: We demonstrated that the influences of diets on the intestinal microbiome could be observed in young beef cattle after weaning. A significantly higher level of microbial diversity was evidenced in feces of grass-fed animals comparing to grain-fed cattle. Top 20 essential genera were obtained with random forest analysis on the fecal microbiome to identify candidate microbial biomarkers. Moreover, the jejunal microbiome of adult Angus beef cattle exhibited significant differences in microbial composition and metabolic potential under different diets. Global balances and microbiome signatures of bile acids were identified.
Conclusions: The findings from this study provided deep insights into microbiome compositions in fecal and jejunal and identified relationships between jejunal microbiome and bile acids with diets in Angus beef cattle. Our results should help us better understand grass-fed beef in production practice and potential health benefits.
Keywords
16S rRNA gene sequencing, Angus beef cattle, Grass-fed beef, Jejunal microbiome, Microbiome signatures, Bile acids
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CURRENT STATUS: Published
View the published article at Animal Microbiome.
No community comments so far
Editor assigned
On 31 Aug, 2020
Editorial decision: Accept
On 31 Aug, 2020
Submission checks complete
On 30 Aug, 2020
Editor invited
On 30 Aug, 2020
No comments provided
Review #2 received
Received 29 Jul, 2020
Editorial decision: Minor revision
On 29 Jul, 2020
Review #1 received
Received 28 Jul, 2020
Reviewer #2 agreed
On 22 Jul, 2020
Reviewer #1 agreed
On 20 Jul, 2020
Reviewers invited
Invitations sent on 19 Jul, 2020
Submission checks complete
On 17 Jul, 2020
Editor invited
On 17 Jul, 2020
Editor assigned
On 17 Jul, 2020
Version 1
Posted 08 Apr, 2020
No comments provided
Editorial decision: Major revision
On 29 Apr, 2020
Review #1 received
Received 27 Apr, 2020
Review #2 received
Received 23 Apr, 2020
Reviewer #2 agreed
On 14 Apr, 2020
Reviewers invited
Invitations sent on 13 Apr, 2020
Reviewer #1 agreed
On 13 Apr, 2020
Editor assigned
On 06 Apr, 2020
Submission checks complete
On 05 Apr, 2020
Editor invited
On 05 Apr, 2020
First submitted
On 02 Apr, 2020
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A new preprint design is coming!
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See the published version of this preprint at Animal Microbiome.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Animal Microbiome.
No community comments so far
Editor assigned
On 31 Aug, 2020
Editorial decision: Accept
On 31 Aug, 2020
Submission checks complete
On 30 Aug, 2020
Editor invited
On 30 Aug, 2020
No comments provided
Review #2 received
Received 29 Jul, 2020
Editorial decision: Minor revision
On 29 Jul, 2020
Review #1 received
Received 28 Jul, 2020
Reviewer #2 agreed
On 22 Jul, 2020
Reviewer #1 agreed
On 20 Jul, 2020
Reviewers invited
Invitations sent on 19 Jul, 2020
Submission checks complete
On 17 Jul, 2020
Editor invited
On 17 Jul, 2020
Editor assigned
On 17 Jul, 2020
Version 1
Posted 08 Apr, 2020
No comments provided
Editorial decision: Major revision
On 29 Apr, 2020
Review #1 received
Received 27 Apr, 2020
Review #2 received
Received 23 Apr, 2020
Reviewer #2 agreed
On 14 Apr, 2020
Reviewers invited
Invitations sent on 13 Apr, 2020
Reviewer #1 agreed
On 13 Apr, 2020
Editor assigned
On 06 Apr, 2020
Submission checks complete
On 05 Apr, 2020
Editor invited
On 05 Apr, 2020
First submitted
On 02 Apr, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Animal Microbiome.
Background: The small intestine, while serving as the main absorption organ, also possesses a unique microbiome environment and holds the critical function of conversion of primary bile acids. Bile acids are, in turn, able to regulate microbiome structure and promote the growth of bacteria that convert primary bile acids to secondary bile acids. However, in beef cattle, few studies have explored the microbiome compositions of the jejunum and its relationships with bile acids. Therefore, the hypothesis is that microbiome compositions in the intestine are influenced by diet style and also related to bile acids.
Results: We demonstrated that the influences of diets on the intestinal microbiome could be observed in young beef cattle after weaning. A significantly higher level of microbial diversity was evidenced in feces of grass-fed animals comparing to grain-fed cattle. Top 20 essential genera were obtained with random forest analysis on the fecal microbiome to identify candidate microbial biomarkers. Moreover, the jejunal microbiome of adult Angus beef cattle exhibited significant differences in microbial composition and metabolic potential under different diets. Global balances and microbiome signatures of bile acids were identified.
Conclusions: The findings from this study provided deep insights into microbiome compositions in fecal and jejunal and identified relationships between jejunal microbiome and bile acids with diets in Angus beef cattle. Our results should help us better understand grass-fed beef in production practice and potential health benefits.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
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