A wide range of chemicals were measured in drinking water and urine samples through targeted and non-target screening (NTS) methods to estimate human exposure. Tap water samples were collected from 42 locations in Barcelona (Spain) in August-October 2020 and 27 May 2021. Additionally, tap water samples filtered with domestic activated carbon filters (AC, N=6) and reverse osmosis (RO, N=5), commercial bottled water (N=10), and urine samples (N=39) were included. A total of 35 per- and polyfluoroalkyl substances (PFAS) including emerging ether-PFAS, bisphenol A and nonylphenol were analyzed using LC–MS/MS and GC–MS/MS, respectively, and NTS was applied through LC–HRMS. Bisphenol A and nonylphenol were absent in all drinking water samples, 10 PFAS were detected in 69-79% non-filtered tap water, and 5 PFAS were detected in 13% urine samples. PFAS were removed by RO and not by AC filters. NTS tentatively identified pharmaceuticals and other industrial chemicals in drinking water samples.