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Background Landscape industry has been recognized as the "eternal sunrise industry", however, the introduction and cultivation of colored-leaf trees in southern China is relatively vacant.
Results The results showed that the induction effect of the stem was the best for the embryogenic callus, and the regenerated plant with genetic stability was obtained. The MS (Murashige&Skoog) +0.8 mg/L TDZ (Thidiazuron) +1.0 mg/L 6-BA (6-Benzylaminopurine) +0.5 mg/L NAA (1-naphthlcetic acid) +35 g/L sucrose+7.5 g/L semi-fixed medium was the best for callus formation. When selecting type VI callus as embryonic callus induction material, MS+0.6 mg/L TDZ+0.5 mg/L 6-BA +2.0 mg/L IAA (Indole-3-acetic acid) +35 g/L sucrose+7.5 g/L semi-fixed medium can get embryonic callus. The optimal medium for adventitious bud induction is MS+1.0 mg/L TDZ+3.0 mg/L 6-BA+0.2 mg/L NAA+1.2 mg/L IAA+35 g/L sucrose+7.5 g/L semi-fixed medium.The induction rate of adventitious roots in MS+0.6 mg/L TDZ+1.0 mg/L 6-BA+3 mg/L NAA+35 g/L sucrose+7.5 g/L semi-fixed medium was the highest, reaching 76%.
Conclusion In the course of our research, we found that PGRs play an important role in the callus induction stage, and the effect of TDZ is particularly obvious; The callus cells grow and proliferate according to the "S" growth curve, and can be subcultured when the highest growth point is reached to maintain the rapid proliferation of the callus cells and to avoid inactivation of callus caused by tight niche.
Keywords
Acer rubrum L. "October Glory ", tissue culture, embryogenic callus, regeneration
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View the published article at BMC Plant Biology.
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Editor assigned
On 28 May, 2020
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On 27 May, 2020
Editor invited
On 27 May, 2020
Version 1
Posted 31 Mar, 2020
No comments provided
Editorial decision: Major revision
On 27 Apr, 2020
Review #2 received
Received 26 Apr, 2020
Review #3 received
Received 26 Apr, 2020
Reviewer #3 agreed
On 06 Apr, 2020
Reviewer #4 agreed
On 06 Apr, 2020
Review #1 received
Received 04 Apr, 2020
Reviewer #2 agreed
On 03 Apr, 2020
Reviewers invited
Invitations sent on 03 Apr, 2020
Reviewer #1 agreed
On 03 Apr, 2020
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On 25 Mar, 2020
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On 24 Mar, 2020
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A new preprint design is coming!
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See the published version of this preprint at BMC Plant Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Plant Biology.
No community comments so far
Editor assigned
On 28 May, 2020
Submission checks complete
On 27 May, 2020
Editor invited
On 27 May, 2020
Version 1
Posted 31 Mar, 2020
No comments provided
Editorial decision: Major revision
On 27 Apr, 2020
Review #2 received
Received 26 Apr, 2020
Review #3 received
Received 26 Apr, 2020
Reviewer #3 agreed
On 06 Apr, 2020
Reviewer #4 agreed
On 06 Apr, 2020
Review #1 received
Received 04 Apr, 2020
Reviewer #2 agreed
On 03 Apr, 2020
Reviewers invited
Invitations sent on 03 Apr, 2020
Reviewer #1 agreed
On 03 Apr, 2020
Editor assigned
On 25 Mar, 2020
First submitted
On 24 Mar, 2020
Submission checks complete
On 24 Mar, 2020
Editor invited
On 24 Mar, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Plant Biology.
Background Landscape industry has been recognized as the "eternal sunrise industry", however, the introduction and cultivation of colored-leaf trees in southern China is relatively vacant.
Results The results showed that the induction effect of the stem was the best for the embryogenic callus, and the regenerated plant with genetic stability was obtained. The MS (Murashige&Skoog) +0.8 mg/L TDZ (Thidiazuron) +1.0 mg/L 6-BA (6-Benzylaminopurine) +0.5 mg/L NAA (1-naphthlcetic acid) +35 g/L sucrose+7.5 g/L semi-fixed medium was the best for callus formation. When selecting type VI callus as embryonic callus induction material, MS+0.6 mg/L TDZ+0.5 mg/L 6-BA +2.0 mg/L IAA (Indole-3-acetic acid) +35 g/L sucrose+7.5 g/L semi-fixed medium can get embryonic callus. The optimal medium for adventitious bud induction is MS+1.0 mg/L TDZ+3.0 mg/L 6-BA+0.2 mg/L NAA+1.2 mg/L IAA+35 g/L sucrose+7.5 g/L semi-fixed medium.The induction rate of adventitious roots in MS+0.6 mg/L TDZ+1.0 mg/L 6-BA+3 mg/L NAA+35 g/L sucrose+7.5 g/L semi-fixed medium was the highest, reaching 76%.
Conclusion In the course of our research, we found that PGRs play an important role in the callus induction stage, and the effect of TDZ is particularly obvious; The callus cells grow and proliferate according to the "S" growth curve, and can be subcultured when the highest growth point is reached to maintain the rapid proliferation of the callus cells and to avoid inactivation of callus caused by tight niche.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
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