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Research article
Bita Bakhshi
Tarbiat Modares University Faculty of Medical Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7523-7905
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Aims: The aim of this study was to assess the distribution of GBS-related lipo-oligosaccharide (LOS) classes and capsular genotypes among Iranian clinical C. jejuni strains and to assess its relation with dnaK gene expression. Moreover, a comprehensive study of C. jejuni MLST-genotypes and global comparison with peer sequences worldwide was intended.
Methods: Distribution of sialylated-LOS classes and specific capsular genotypes were investigated in C.jejuni of clinical origin. The expression of dnaK in C. jejuni strains was measured by Real-Time-PCR. MLST-genotyping was performed to investigate the clonal relationship of clinical C. jejuni strains and comparison with global sequences worldwide.
Results: C. jejuni HS23/36c was the predominant genotype (45%), followed by HS2 (20%), and HS19 and HS4 (each 10%). A total of 80% of isolates were assigned to LOS class B and C. Higher expression level of dnaK gene was detected in strains with HS23/36c, HS2 and HS4 capsular genotypes and sialylated LOS classes B or C in this study. MLST analysis showed that isolates were highly diverse and represented 6 different sequence types and 3 clonal complexes. ST-21 and ST-257 complexes were dominants (75%) in our C.jejuni strains. The CC21 was the largest CC in our collection which is consistent with global C.jejuni strains worldwide. No new ST and no common ST with our neighbor countries was detected in this study.
Conclusion: Global analysis of MLST results demonstrated that ST-50 (CC21) was widely distributed in different countries, while ST-19 (CC21) and ST-257 (CC257) was less ubiquitously spread. Overall, CC21 and CC353 complexes were most frequent and most widely distributed clonal complexes around the world; although, CC353 was not detected in this study. This shows a picture of movement of dominant Campylobacter strains worldwide. The occurrence of identical clonal complexes with different capsular types and LOS classes in this study is consistent with genetic variation in circulating identical genotypes and their evolution toward different pathotypes probably through acquisition of different genetic elements including LOS and CPS gene clusters.
Keywords
C. jejuni, LOS class, Capsular genotype, dnaK, MLST, Iran.
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On 06 Jan, 2021
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Editorial decision: Minor revision
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On 01 Jan, 2021
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On 01 Jan, 2021
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Editorial decision: Minor revision
On 29 Dec, 2020
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On 26 Dec, 2020
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Editor invited
On 26 Dec, 2020
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Posted 09 Dec, 2020
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On 09 Dec, 2020
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On 09 Dec, 2020
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Version 1
Posted 04 Aug, 2020
No comments provided
Editorial decision: Major revision
On 21 Oct, 2020
Review #4 received
Received 21 Sep, 2020
Reviewer #3 agreed
On 20 Sep, 2020
Reviewer #4 agreed
On 20 Sep, 2020
Review #3 received
Received 20 Sep, 2020
Review #2 received
Received 15 Sep, 2020
Review #1 received
Received 15 Aug, 2020
Reviewer #2 agreed
On 10 Aug, 2020
Reviewer #1 agreed
On 07 Aug, 2020
Reviewers invited
Invitations sent on 04 Aug, 2020
Editor assigned
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A new preprint design is coming!
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See the published version of this preprint at BMC Infectious Diseases.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Bita Bakhshi
Tarbiat Modares University Faculty of Medical Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7523-7905
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Infectious Diseases.
No community comments so far
Submission checks complete
On 07 Jan, 2021
Editorial decision: Accept
On 06 Jan, 2021
Version (no preprint)
Posted 03 Jan, 2021
Editorial decision: Minor revision
On 03 Jan, 2021
Editor assigned
On 01 Jan, 2021
Submission checks complete
On 01 Jan, 2021
Editor invited
On 01 Jan, 2021
This version was not preprinted
Version (no preprint)
Posted 29 Dec, 2020
Editorial decision: Minor revision
On 29 Dec, 2020
Editor assigned
On 26 Dec, 2020
Submission checks complete
On 26 Dec, 2020
Editor invited
On 26 Dec, 2020
This version was not preprinted
Version (no preprint)
Posted 09 Dec, 2020
Editor assigned
On 09 Dec, 2020
Submission checks complete
On 09 Dec, 2020
Editor invited
On 09 Dec, 2020
This version was not preprinted
Version 1
Posted 04 Aug, 2020
No comments provided
Editorial decision: Major revision
On 21 Oct, 2020
Review #4 received
Received 21 Sep, 2020
Reviewer #3 agreed
On 20 Sep, 2020
Reviewer #4 agreed
On 20 Sep, 2020
Review #3 received
Received 20 Sep, 2020
Review #2 received
Received 15 Sep, 2020
Review #1 received
Received 15 Aug, 2020
Reviewer #2 agreed
On 10 Aug, 2020
Reviewer #1 agreed
On 07 Aug, 2020
Reviewers invited
Invitations sent on 04 Aug, 2020
Editor assigned
On 30 Jul, 2020
Submission checks complete
On 29 Jul, 2020
Editor invited
On 29 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Infectious Diseases
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Infectious Diseases.
Aims: The aim of this study was to assess the distribution of GBS-related lipo-oligosaccharide (LOS) classes and capsular genotypes among Iranian clinical C. jejuni strains and to assess its relation with dnaK gene expression. Moreover, a comprehensive study of C. jejuni MLST-genotypes and global comparison with peer sequences worldwide was intended.
Methods: Distribution of sialylated-LOS classes and specific capsular genotypes were investigated in C.jejuni of clinical origin. The expression of dnaK in C. jejuni strains was measured by Real-Time-PCR. MLST-genotyping was performed to investigate the clonal relationship of clinical C. jejuni strains and comparison with global sequences worldwide.
Results: C. jejuni HS23/36c was the predominant genotype (45%), followed by HS2 (20%), and HS19 and HS4 (each 10%). A total of 80% of isolates were assigned to LOS class B and C. Higher expression level of dnaK gene was detected in strains with HS23/36c, HS2 and HS4 capsular genotypes and sialylated LOS classes B or C in this study. MLST analysis showed that isolates were highly diverse and represented 6 different sequence types and 3 clonal complexes. ST-21 and ST-257 complexes were dominants (75%) in our C.jejuni strains. The CC21 was the largest CC in our collection which is consistent with global C.jejuni strains worldwide. No new ST and no common ST with our neighbor countries was detected in this study.
Conclusion: Global analysis of MLST results demonstrated that ST-50 (CC21) was widely distributed in different countries, while ST-19 (CC21) and ST-257 (CC257) was less ubiquitously spread. Overall, CC21 and CC353 complexes were most frequent and most widely distributed clonal complexes around the world; although, CC353 was not detected in this study. This shows a picture of movement of dominant Campylobacter strains worldwide. The occurrence of identical clonal complexes with different capsular types and LOS classes in this study is consistent with genetic variation in circulating identical genotypes and their evolution toward different pathotypes probably through acquisition of different genetic elements including LOS and CPS gene clusters.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
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