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Research Article
Michał Kwiatek
Instytut Genetyki Roslin Polskiej Akademii Nauk
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9442-3124
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Triticale (×Triticosecale Wittmack) is a commercial hybrid harboring wheat (Triticum sp.) and rye (Secale cereale L.) genomes. The limited genetic diversity of this crop resulted in the collapse of fungal disease resistance. Leaf rust disease, caused by Puccinia triticina Eriks. is reported to reduce the triticale yield significantly (more than 30%). There is a need to enlarge the genetic variability of this crop including leaf resistance genes. The main aim of this research was to transfer Lr39 and Lr54 leaf rust resistance genes into triticale from Aegilops tauschii and Ae. kotschyi, respectively. A reaction of seedlings of 200 plants of two triticale-Aegilops translocation lines (Bogo-2Dt.2R and Sekundo-2Sk.2R) was compared after inoculation with a natural mixture of P. triticina races, specific to triticale. Before inoculation, each plant was screened using molecular cytogenetics and molecular markers linked to leaf rust resistance genes. Presence of Aegilops chromosome segments was confirmed using genomic in situ hybridization (GISH). Lr39 and Lr54 leaf rust resistance genes were identified using Xgdm35 and S14 molecular markers, respectively. After inoculation, a significant improvement of resistance severity was observed in Sekundo-2Sk.2R in comparison with triticale cv. Sekundo plants. The resistance level of Bogo-2Dt.2R did not differ compared to triticale cv. Bogo plants. It was shown, that Lr39 gene did not increase the leaf rust resistance level of triticale cv. Bogo.
Keywords
Aegilops, genomic in situ hybridization, leaf rust, molecular markers, resistance genes, triticale
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
- SupportingInformation1.docx
Supporting Information 1. Analysis of variance (ANOVA) and Tukey’s HSD test for leaf rust infection scores (independent samples) of three groups of plants: 1) triticale cv. Bogo, 2) triticale cv. Sekundo and 3) wheat cv. Michgan Amber. HSD - the absolute (unsigned) difference between any two sample means required for significance at the designated level HSD[.05] for the .05 level; HSD[.01] for the .01 level.
- SupportingInformation2.docx
Supporting Information 2. Analysis of variance (ANOVA) and Tukey’s HSD test for leaf rust infection scores (independent samples) of Bogo-2Dt.2R translocation line after: 1) 5, 2) 10 and 3) 15 days post inoculation (dpi). HSD - the absolute (unsigned) difference between any two sample means required for significance at the designated level HSD[.05] for the .05 level; HSD[.01] for the .01 level.
- SupportingInformation3.docx
Supporting Information 3. Analysis of variance (ANOVA) and Tukey’s HSD test for leaf rust infection scores (independent samples) of Sekundo-2Sk.2R translocation line after: 1) 5, 2) 10 and 3) 15 days post inoculation (dpi).
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CURRENT STATUS: Under Review
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Posted 18 Feb, 2021
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Received 08 Feb, 2021
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Subject Areas
Molecular Genetics
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This preprint is under consideration at Journal of Applied Genetics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Michał Kwiatek
Instytut Genetyki Roslin Polskiej Akademii Nauk
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9442-3124
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 18 Feb, 2021
No community comments so far
Reviews received
Received 08 Feb, 2021
Reviewers invited
Invitations sent on 05 Feb, 2021
Editor assigned
On 03 Feb, 2021
First submitted
On 03 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Molecular Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Triticale (×Triticosecale Wittmack) is a commercial hybrid harboring wheat (Triticum sp.) and rye (Secale cereale L.) genomes. The limited genetic diversity of this crop resulted in the collapse of fungal disease resistance. Leaf rust disease, caused by Puccinia triticina Eriks. is reported to reduce the triticale yield significantly (more than 30%). There is a need to enlarge the genetic variability of this crop including leaf resistance genes. The main aim of this research was to transfer Lr39 and Lr54 leaf rust resistance genes into triticale from Aegilops tauschii and Ae. kotschyi, respectively. A reaction of seedlings of 200 plants of two triticale-Aegilops translocation lines (Bogo-2Dt.2R and Sekundo-2Sk.2R) was compared after inoculation with a natural mixture of P. triticina races, specific to triticale. Before inoculation, each plant was screened using molecular cytogenetics and molecular markers linked to leaf rust resistance genes. Presence of Aegilops chromosome segments was confirmed using genomic in situ hybridization (GISH). Lr39 and Lr54 leaf rust resistance genes were identified using Xgdm35 and S14 molecular markers, respectively. After inoculation, a significant improvement of resistance severity was observed in Sekundo-2Sk.2R in comparison with triticale cv. Sekundo plants. The resistance level of Bogo-2Dt.2R did not differ compared to triticale cv. Bogo plants. It was shown, that Lr39 gene did not increase the leaf rust resistance level of triticale cv. Bogo.
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
- SupportingInformation1.docx
Supporting Information 1. Analysis of variance (ANOVA) and Tukey’s HSD test for leaf rust infection scores (independent samples) of three groups of plants: 1) triticale cv. Bogo, 2) triticale cv. Sekundo and 3) wheat cv. Michgan Amber. HSD - the absolute (unsigned) difference between any two sample means required for significance at the designated level HSD[.05] for the .05 level; HSD[.01] for the .01 level.
- SupportingInformation2.docx
Supporting Information 2. Analysis of variance (ANOVA) and Tukey’s HSD test for leaf rust infection scores (independent samples) of Bogo-2Dt.2R translocation line after: 1) 5, 2) 10 and 3) 15 days post inoculation (dpi). HSD - the absolute (unsigned) difference between any two sample means required for significance at the designated level HSD[.05] for the .05 level; HSD[.01] for the .01 level.
- SupportingInformation3.docx
Supporting Information 3. Analysis of variance (ANOVA) and Tukey’s HSD test for leaf rust infection scores (independent samples) of Sekundo-2Sk.2R translocation line after: 1) 5, 2) 10 and 3) 15 days post inoculation (dpi).
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