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Research Article
Dahai Wei
Jiaxing University
Corresponding Author
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Background: Schistosoma japonicum (S. japonicum) is a parasitic flatworm that is the aetiological agent of human schistosomiasis, an important cause of hepatic fibrosis. Schistosomiasis-induced hepatic fibrosis is a consequence of the highly fibrogenic nature of egg-induced granulomatuous lesions, the main pathogenic factor of schistosomiasis. Although global awareness of the association between schistosomiasis-indued hepatic fibrosis and s. japonicum infection is increasing, little is known about the mechanism mediating the rapid progression to schistosomiasis in cirrhotic patients.
Methods: We systematically used data-independent acquisition (DIA)-based liquid chromatography-mass spectrometry to identify differentially expressed proteins in serum samples from patients with advanced S. japonicum-induced hepatic fibrosis.
Results: On the basis of our analysis, we identified 1,144 proteins, among which 67 were differentially expressed between the healthy control and SHF-F2 groups and 214 were differentially expressed between the SHF-F2 and SHF-F4 groups (up- or downregulation of at least 1.5-fold in serum samples). Furthermore, our results indicated that two selected proteins (C1QA and CFD) are potential biomarkers for distinguishing patients with cirrhosis resulting from S. japonicum infection.
Conclusions: This report is the first to provide a global proteomic profile of serum samples from patients with advanced S. japonicum-induced hepatic fibrosis. Our results shed new light on the molecular mechanisms that are dysregulated in and contribute to the pathogenesis of schistosomiasis-induced hepatic fibrosis. C1QA and CFD are promising diagnostic markers for patients with cirrhosis resulting from S. japonicum infection, although further large-scale studies are needed. Our DIA-based quantitative proteomic analysis revealed molecular differences among individuals with different stages of advanced S. japonicum-induced hepatic fibrosis and might provide fundamental information for further detailed investigations.
Keywords
Quantitative proteomics, SHF, Schistosoma japonicum, C1QA, CFD
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This is a list of supplementary files associated with this preprint. Click to download.
- Table1.xlsx
- Additionalfile1S1S2S3andS4.xlsx
Additional file1: Table S1. Differentially expressed proteins of serum samples between SHF with F2 and healthy controls (sheet 1). Table S2. Differentially expressed proteins of serum samples between SHF with F4 and SHF with F2 (sheet 2). Table S3. KEGG pathway analysis of the differentially expressed proteins of serum samples between SHF with F2 and healthy controls (fold change of ≥± 1.5) (sheet 3). Table S4. KEGG pathway analysis of the differentially expressed proteins of serum samples between SHF with F4 and SHF with F2 (fold change of ≥± 1.5) (sheet 4).
- Additionalfile2.docx
Additional file2: Figure S1. Overall technical route of this project. Figure S2. The qualities of the proteome dataset. (A) Quantitative heatmap of DIA. (B) The scores of Principle Component Ananlysis (PCA). (C) Chart of iRT elution time. (D) Column peak capacity statistics. (E) Protein scores of FDR. (F) The scatterplot of QC. Figure S3. Distribution analysis of differentially expressed proteins. (A) The protein ratio distribution between the healthy control individual and SHF-F2 patient serum samples. (B) The protein ratio distribution between SHF-F4 and SHF-F2 patient serum samples.
- Graphicalabstract.tif
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View the published article at Parasites & Vectors.
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A new preprint design is coming!
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See the published version of this preprint at Parasites & Vectors.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Dahai Wei
Jiaxing University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Parasites & Vectors.
Version 1
Posted 10 Feb, 2021
No community comments so far
Editorial decision: Major Revision
On 26 Feb, 2021
Review #2 received
Received 25 Feb, 2021
Review #1 received
Received 16 Feb, 2021
Reviewer #2 agreed
On 09 Feb, 2021
Reviewers invited
Invitations sent on 04 Feb, 2021
Reviewer #1 agreed
On 04 Feb, 2021
Reviews received
Received 04 Feb, 2021
Editor invited
On 03 Feb, 2021
Editor assigned
On 03 Feb, 2021
Submission checks complete
On 03 Feb, 2021
First submitted
On 03 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Parasites & Vectors.
Background: Schistosoma japonicum (S. japonicum) is a parasitic flatworm that is the aetiological agent of human schistosomiasis, an important cause of hepatic fibrosis. Schistosomiasis-induced hepatic fibrosis is a consequence of the highly fibrogenic nature of egg-induced granulomatuous lesions, the main pathogenic factor of schistosomiasis. Although global awareness of the association between schistosomiasis-indued hepatic fibrosis and s. japonicum infection is increasing, little is known about the mechanism mediating the rapid progression to schistosomiasis in cirrhotic patients.
Methods: We systematically used data-independent acquisition (DIA)-based liquid chromatography-mass spectrometry to identify differentially expressed proteins in serum samples from patients with advanced S. japonicum-induced hepatic fibrosis.
Results: On the basis of our analysis, we identified 1,144 proteins, among which 67 were differentially expressed between the healthy control and SHF-F2 groups and 214 were differentially expressed between the SHF-F2 and SHF-F4 groups (up- or downregulation of at least 1.5-fold in serum samples). Furthermore, our results indicated that two selected proteins (C1QA and CFD) are potential biomarkers for distinguishing patients with cirrhosis resulting from S. japonicum infection.
Conclusions: This report is the first to provide a global proteomic profile of serum samples from patients with advanced S. japonicum-induced hepatic fibrosis. Our results shed new light on the molecular mechanisms that are dysregulated in and contribute to the pathogenesis of schistosomiasis-induced hepatic fibrosis. C1QA and CFD are promising diagnostic markers for patients with cirrhosis resulting from S. japonicum infection, although further large-scale studies are needed. Our DIA-based quantitative proteomic analysis revealed molecular differences among individuals with different stages of advanced S. japonicum-induced hepatic fibrosis and might provide fundamental information for further detailed investigations.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- Table1.xlsx
- Additionalfile1S1S2S3andS4.xlsx
Additional file1: Table S1. Differentially expressed proteins of serum samples between SHF with F2 and healthy controls (sheet 1). Table S2. Differentially expressed proteins of serum samples between SHF with F4 and SHF with F2 (sheet 2). Table S3. KEGG pathway analysis of the differentially expressed proteins of serum samples between SHF with F2 and healthy controls (fold change of ≥± 1.5) (sheet 3). Table S4. KEGG pathway analysis of the differentially expressed proteins of serum samples between SHF with F4 and SHF with F2 (fold change of ≥± 1.5) (sheet 4).
- Additionalfile2.docx
Additional file2: Figure S1. Overall technical route of this project. Figure S2. The qualities of the proteome dataset. (A) Quantitative heatmap of DIA. (B) The scores of Principle Component Ananlysis (PCA). (C) Chart of iRT elution time. (D) Column peak capacity statistics. (E) Protein scores of FDR. (F) The scatterplot of QC. Figure S3. Distribution analysis of differentially expressed proteins. (A) The protein ratio distribution between the healthy control individual and SHF-F2 patient serum samples. (B) The protein ratio distribution between SHF-F4 and SHF-F2 patient serum samples.
- Graphicalabstract.tif
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