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Research
Shuo Wang
Beijing Tiantan Hospital
Corresponding Author
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Background
Recently, abundant numbers of studies have revealed many functions of circular RNAs in multiple diseases, however, the role of circular RNA in the rupture of human intracranial aneurysm is still unknown. This study aims to explore the potential functions of circular RNA in the rupture of human intracranial aneurysms.
Results
The differentially expressed circular RNAs between un-ruptured intracranial aneurysms (n = 5) and ruptured intracranial aneurysms (n = 5) were analyzed with the Arraystar human circRNAs microarray and total of 13175 circular RNAs were detected. Among them 63 circular RNAs upregulated and 54 circular RNAs downregulated significantly in ruptured intracranial aneurysms compared with un-ruptured intracranial aneurysms (p < 0.05, Fold Change > 1.5). 5 upregulated circular RNAs were selected for further study (hsa_circ_0001947, hsa_circ_0043001, hsa_circ_0064557, hsa_circ_0058514, hsa_circ_0005505). The results of qPCR showed only hsa_circ_0005505 significantly upregulated (P < 0.05). The expression of hsa_circ_0005505 was higher in ruptured intracranial aneurysm tissues. And our in vitro data showed that hsa_circRNA_005505 promotes the proliferation the migration and apoptosis of vascular smooth muscle cell.
Conclusions
This study revealed an important role of hsa_circ_0005505 in the proliferation, migration and apoptosis of vascular smooth muscle cell, and indicated that hsa_circ_0005505 may associated with the pathological process of intracranial aneurysms.
Keywords
circular RNA, intracranial aneurysm, vascular smooth muscle cells, phenotype modulation
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.pdf
Additional file 1: RNA quantification and quality assurance by NanoDrop ND-1000
- Additionalfile2.pdf
Additional file 2: Labeling efficiency
- Additionalfile3.docx
Additional file 3: qPCR primer sequences
- Additionalfile4.xls
Additional file 4: Significant differentially expressed circRNAs (FC≥1.5 and P≤0.05)
- Additionalfile5.xls
Additional file 5: MicroRNAs binding on hsa_circ_0005505.
- Additionalfile6.xls
Additional file 6：Target genes of microRNAs binding on hsa_circ_0005505.
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Shuo Wang
Beijing Tiantan Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 22 Feb, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Recently, abundant numbers of studies have revealed many functions of circular RNAs in multiple diseases, however, the role of circular RNA in the rupture of human intracranial aneurysm is still unknown. This study aims to explore the potential functions of circular RNA in the rupture of human intracranial aneurysms.
Results
The differentially expressed circular RNAs between un-ruptured intracranial aneurysms (n = 5) and ruptured intracranial aneurysms (n = 5) were analyzed with the Arraystar human circRNAs microarray and total of 13175 circular RNAs were detected. Among them 63 circular RNAs upregulated and 54 circular RNAs downregulated significantly in ruptured intracranial aneurysms compared with un-ruptured intracranial aneurysms (p < 0.05, Fold Change > 1.5). 5 upregulated circular RNAs were selected for further study (hsa_circ_0001947, hsa_circ_0043001, hsa_circ_0064557, hsa_circ_0058514, hsa_circ_0005505). The results of qPCR showed only hsa_circ_0005505 significantly upregulated (P < 0.05). The expression of hsa_circ_0005505 was higher in ruptured intracranial aneurysm tissues. And our in vitro data showed that hsa_circRNA_005505 promotes the proliferation the migration and apoptosis of vascular smooth muscle cell.
Conclusions
This study revealed an important role of hsa_circ_0005505 in the proliferation, migration and apoptosis of vascular smooth muscle cell, and indicated that hsa_circ_0005505 may associated with the pathological process of intracranial aneurysms.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.pdf
Additional file 1: RNA quantification and quality assurance by NanoDrop ND-1000
- Additionalfile2.pdf
Additional file 2: Labeling efficiency
- Additionalfile3.docx
Additional file 3: qPCR primer sequences
- Additionalfile4.xls
Additional file 4: Significant differentially expressed circRNAs (FC≥1.5 and P≤0.05)
- Additionalfile5.xls
Additional file 5: MicroRNAs binding on hsa_circ_0005505.
- Additionalfile6.xls
Additional file 6：Target genes of microRNAs binding on hsa_circ_0005505.
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