Enhanced Virulence of Salmonella enterica serovar Enteritidis ATCC13076 under Acid Stress by Global Transcriptomics
Salmonella enterica serovar Enteritidis is a primary pathogen causing foodborne diseases and intestinal inflammatory responses. Acid tolerance response (ATR), as a strategy of adaption and resistance to acid stress, may contribute to enhanced virulence. In this study, there was a moderately acid adaption (pH 5.0) for S. Enteritidis cells prior to treatment with acid stress (pH 3.0). To figure out whether S. Enteritidis up-regulated the virulence or not, a global transcriptomic analysis was carried out by high-throughout RNA-sEq. The results showed 74 differentially expressed genes (DEGs) involved in virulence were identified after acid stress, among which, 62 DEGs were up-regulated and 12 DEGs were down-regulated. Afterwards, those virulence-linked DEGs were discussed and classified into four aspects based on the steps of infection, including flagellar functions, fimbrial adhesins, T3SS-mediated invasion and other virulent determinants. In conclusion, S. Enteritidis seemed to exhibit a trend of virulent genes towards high-expression under acid stress, revealing risks of Salmonella in acid-containing food. To our knowledge, there were few studies on comprehensively analyzing virulent genes expression changes of Salmonella, but it’s novel to put forward pathogenicity as the highest priority under acid environment.
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Posted 23 Feb, 2021
Invitations sent on 18 Feb, 2021
Received 18 Feb, 2021
On 16 Feb, 2021
On 06 Feb, 2021
Enhanced Virulence of Salmonella enterica serovar Enteritidis ATCC13076 under Acid Stress by Global Transcriptomics
Posted 23 Feb, 2021
Invitations sent on 18 Feb, 2021
Received 18 Feb, 2021
On 16 Feb, 2021
On 06 Feb, 2021
Salmonella enterica serovar Enteritidis is a primary pathogen causing foodborne diseases and intestinal inflammatory responses. Acid tolerance response (ATR), as a strategy of adaption and resistance to acid stress, may contribute to enhanced virulence. In this study, there was a moderately acid adaption (pH 5.0) for S. Enteritidis cells prior to treatment with acid stress (pH 3.0). To figure out whether S. Enteritidis up-regulated the virulence or not, a global transcriptomic analysis was carried out by high-throughout RNA-sEq. The results showed 74 differentially expressed genes (DEGs) involved in virulence were identified after acid stress, among which, 62 DEGs were up-regulated and 12 DEGs were down-regulated. Afterwards, those virulence-linked DEGs were discussed and classified into four aspects based on the steps of infection, including flagellar functions, fimbrial adhesins, T3SS-mediated invasion and other virulent determinants. In conclusion, S. Enteritidis seemed to exhibit a trend of virulent genes towards high-expression under acid stress, revealing risks of Salmonella in acid-containing food. To our knowledge, there were few studies on comprehensively analyzing virulent genes expression changes of Salmonella, but it’s novel to put forward pathogenicity as the highest priority under acid environment.
Figure 1
Figure 2
Figure 3
Figure 4