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Research
Environmental shaping of the bacterial and fungal community in infant bed dust and correlations with the airway microbiota
Søren Johannes Sørensen
University of Copenhagen, Faculty of Science
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6227-9906
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background From early life children are exposed to a multitude of environmental exposures, which may be of crucial importance for a healthy development. Here, the environmental microbiota may be of particular interest as it represents the interface between environmental factors and the child. As infants in modern societies spend a considerable amount of time indoors, we hypothesize that the indoor bed dust microbiota might be an important factor for the child and for the colonization of the early airway microbiome. To explore this hypothesis, we analysed the influence of environmental exposures on 577 dust samples from children’s beds (age 6 months) together with 542 airway samples (age 3 months) from the Copenhagen Prospective Studies on Asthma in Childhood 2010 cohort. Results The bed dust and airway microbiota were both profiled with bacterial amplicon sequencing while also fungal community was profiled from bed dust. Bacterial and fungal diversity in the dust was positively correlated. We observed that bacterial bed dust microbiota was influenced by multiple environmental factors, such as type of home (house or apartment), rural or urban living environment and pets (cat and/or dog), whereas fungal bed dust microbiota was majorly influenced by the sampling season. We further observed minor associations between bed dust and airway microbiota compositions among infants, but no evidence of transfer of individual taxa between the two departments.
Conclusions Our finding demonstrate that bed dust microbiota is influenced by environmental exposures and could represent an interface between environment and child.
Keywords
House dust, bacterial microbiome, fungal microbiome, airway microbiome, infant microbiome
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This is a preprint. It has not completed peer review.
Research
Environmental shaping of the bacterial and fungal community in infant bed dust and correlations with the airway microbiota
Søren Johannes Sørensen
University of Copenhagen, Faculty of Science
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6227-9906
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 14 Apr, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background From early life children are exposed to a multitude of environmental exposures, which may be of crucial importance for a healthy development. Here, the environmental microbiota may be of particular interest as it represents the interface between environmental factors and the child. As infants in modern societies spend a considerable amount of time indoors, we hypothesize that the indoor bed dust microbiota might be an important factor for the child and for the colonization of the early airway microbiome. To explore this hypothesis, we analysed the influence of environmental exposures on 577 dust samples from children’s beds (age 6 months) together with 542 airway samples (age 3 months) from the Copenhagen Prospective Studies on Asthma in Childhood 2010 cohort. Results The bed dust and airway microbiota were both profiled with bacterial amplicon sequencing while also fungal community was profiled from bed dust. Bacterial and fungal diversity in the dust was positively correlated. We observed that bacterial bed dust microbiota was influenced by multiple environmental factors, such as type of home (house or apartment), rural or urban living environment and pets (cat and/or dog), whereas fungal bed dust microbiota was majorly influenced by the sampling season. We further observed minor associations between bed dust and airway microbiota compositions among infants, but no evidence of transfer of individual taxa between the two departments.
Conclusions Our finding demonstrate that bed dust microbiota is influenced by environmental exposures and could represent an interface between environment and child.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the manuscript can be downloaded and accessed as a PDF.