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Research article
Ultraviolet irradiation improves the hydrophilicity and osteo-conduction of hydroxyapatite
Yuji Yamamoto
Hirosaki Daigaku Igakubu Daigakuin Igaku Kenkyuka
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7472-363X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Journal of Orthopaedic Surgery and Research.
Background: Treating a titanium or titanium alloy implant with ultraviolet (UV) light is known to improve its associated cell growth and osseointegration. However, little is known about the effect of UV irradiation on hydroxyapatite (HA), which is also used frequently in orthopaedic and dental surgery. Here we examined the effect of UV irradiation on the hydrophilicity of HA, and on its osteoconduction ability in rats.
Methods: HA implants of low and high porosity were treated with UV light, and photofunctionalisation was assessed by the contact angle of a water drop on the surface. HA implants were also inserted into rat femurs, and the rats were killed two or four weeks later. The bone volume and bone area ratio were calculated from microcomputed tomography and histological data.
Results: The contact angle of a water drop on HA implants of both porosities was significantly reduced after UV irradiation. In the rat femurs, there was no significant difference in the bone volume between the UV light-treated and control implants at two or four weeks. The bone area ratio for the UV light-treated versus control implants was significantly increased at two weeks, but there was no significant difference at four weeks.
Conclusions: The surface of UV-irradiated HA disks was hydrophilic, in contrast to that of non-irradiated HA disks. Photofunctionalisation accelerated the increase in the bone area ratio in the early healing stage. This technology can be applied to surgical cases requiring the early fusion of bone and HA.
Keywords
Ultraviolet, Hydroxyapatite, Osseointegration, Photofunctionalisation
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CURRENT STATUS: Published
Version 2
Posted 15 Jun, 2020
Published
On 18 Sep, 2020
No community comments so far
Editorial decision: Accept
On 07 Sep, 2020
Editor assigned
On 12 Jun, 2020
Submission checks complete
On 11 Jun, 2020
Editor invited
On 11 Jun, 2020
No comments provided
Editorial decision: Major revision
On 11 May, 2020
Review #1 received
Received 02 May, 2020
Review #3 received
Received 02 May, 2020
Reviewer #4 agreed
On 01 May, 2020
Reviewer #3 agreed
On 29 Apr, 2020
Editor assigned
On 28 Apr, 2020
Reviewers invited
Invitations sent on 28 Apr, 2020
Reviewer #1 agreed
On 28 Apr, 2020
Reviewer #2 agreed
On 28 Apr, 2020
Editor invited
On 27 Apr, 2020
Submission checks complete
On 17 Apr, 2020
First submitted
On 16 Apr, 2020
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This preprint is under consideration at Journal of Orthopaedic Surgery and Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Ultraviolet irradiation improves the hydrophilicity and osteo-conduction of hydroxyapatite
Yuji Yamamoto
Hirosaki Daigaku Igakubu Daigakuin Igaku Kenkyuka
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7472-363X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 15 Jun, 2020
Published
On 18 Sep, 2020
No community comments so far
Editorial decision: Accept
On 07 Sep, 2020
Editor assigned
On 12 Jun, 2020
Submission checks complete
On 11 Jun, 2020
Editor invited
On 11 Jun, 2020
No comments provided
Editorial decision: Major revision
On 11 May, 2020
Review #1 received
Received 02 May, 2020
Review #3 received
Received 02 May, 2020
Reviewer #4 agreed
On 01 May, 2020
Reviewer #3 agreed
On 29 Apr, 2020
Editor assigned
On 28 Apr, 2020
Reviewers invited
Invitations sent on 28 Apr, 2020
Reviewer #1 agreed
On 28 Apr, 2020
Reviewer #2 agreed
On 28 Apr, 2020
Editor invited
On 27 Apr, 2020
Submission checks complete
On 17 Apr, 2020
First submitted
On 16 Apr, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Journal of Orthopaedic Surgery and Research.
Background: Treating a titanium or titanium alloy implant with ultraviolet (UV) light is known to improve its associated cell growth and osseointegration. However, little is known about the effect of UV irradiation on hydroxyapatite (HA), which is also used frequently in orthopaedic and dental surgery. Here we examined the effect of UV irradiation on the hydrophilicity of HA, and on its osteoconduction ability in rats.
Methods: HA implants of low and high porosity were treated with UV light, and photofunctionalisation was assessed by the contact angle of a water drop on the surface. HA implants were also inserted into rat femurs, and the rats were killed two or four weeks later. The bone volume and bone area ratio were calculated from microcomputed tomography and histological data.
Results: The contact angle of a water drop on HA implants of both porosities was significantly reduced after UV irradiation. In the rat femurs, there was no significant difference in the bone volume between the UV light-treated and control implants at two or four weeks. The bone area ratio for the UV light-treated versus control implants was significantly increased at two weeks, but there was no significant difference at four weeks.
Conclusions: The surface of UV-irradiated HA disks was hydrophilic, in contrast to that of non-irradiated HA disks. Photofunctionalisation accelerated the increase in the bone area ratio in the early healing stage. This technology can be applied to surgical cases requiring the early fusion of bone and HA.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7