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Research
Baosen Zhou
Shengjing Hospital of China Medical University
Corresponding Author
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Background
Exosomes play an important role in the regulation of various processes in the tumor microenvironment. In this study, we explored the mechanisms of exosomal miR-149-5p in the pathogenesis of lung adenocarcinoma.
Methods
Raw GEO data were downloaded and normalized using the R package. Exosomal miRNAs that were found to be significantly expressed were subjected to WGCNA co-expression network analysis. The proliferation, migration and apoptotic abilities of tumor cells were assessed by the MTS, Transwell and apoptosis assays. Univariate and multivariate analyses were performed to identify the independent factors of target genes.
Results
Results showed that exosomal miR-149-5p was enriched in peripheral serum and tumor cells. The upregulation of exosomal miR-149-5p promoted the growth and migration of tumor cells, and inhibited apoptosis of tumor cells. Notably, target genes of exosomal miR-149-5p, AMOTL2 and MSRB3, were significantly downregulated in lung adenocarcinoma and thus may be considered as independent risk factors of poor survival. In TCGA-LUAD cohort, miR-149-5p was found to be a negative regulator of AMOTL2 and MSRB3 genes.
Conclusion
Collectively, these results provide novel insights for further mechanistic studies on lung adenocarcinoma.
Keywords
exosomal miR-149-5p, WGCNA, AMOTL2, MSRB3
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Baosen Zhou
Shengjing Hospital of China Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
View the published article at Aging.
Version 1
Posted 17 Apr, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Pulmonology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Aging.
Background
Exosomes play an important role in the regulation of various processes in the tumor microenvironment. In this study, we explored the mechanisms of exosomal miR-149-5p in the pathogenesis of lung adenocarcinoma.
Methods
Raw GEO data were downloaded and normalized using the R package. Exosomal miRNAs that were found to be significantly expressed were subjected to WGCNA co-expression network analysis. The proliferation, migration and apoptotic abilities of tumor cells were assessed by the MTS, Transwell and apoptosis assays. Univariate and multivariate analyses were performed to identify the independent factors of target genes.
Results
Results showed that exosomal miR-149-5p was enriched in peripheral serum and tumor cells. The upregulation of exosomal miR-149-5p promoted the growth and migration of tumor cells, and inhibited apoptosis of tumor cells. Notably, target genes of exosomal miR-149-5p, AMOTL2 and MSRB3, were significantly downregulated in lung adenocarcinoma and thus may be considered as independent risk factors of poor survival. In TCGA-LUAD cohort, miR-149-5p was found to be a negative regulator of AMOTL2 and MSRB3 genes.
Conclusion
Collectively, these results provide novel insights for further mechanistic studies on lung adenocarcinoma.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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