Qualitative and quantitative assay of glucose 6 phosphate dehydrogenase in patients attending tertiary care center
Objectives: The study was carried out with the aim to find out the frequency of Glucose 6 phosphate dehydrogenase (G6PD) deficiency among the patients attending the hospital and to rationalize the qualitative methemoglobin reduction test in reference to the quantitative spectrophotometric assay. Timely screening of the patients for G6PD with appropriate screening method can play an important role in preventing hemolytic crisis that arises from therapeutic use of oxidative drugs like primaquine.
Result: The frequency of G6PD deficient cases was 3% by both of the employed tests. The mean ± SD of G6PD activity in the patients under study was 15.34 ± 4.7 IU/g Hb in males and 16.01 ± 3.74 IU/g Hb in females. G6PD activity was positively associated with reticulocyte count (r = 0.289, p-value = 0.004) and negatively with mean corpuscular hemoglobin concentration (r = -0.220, p-value = 0.028). The correlation of red blood corpuscular count and G6PD was statistically significant (p-value = 0.048).
Posted 26 May, 2020
On 22 Jun, 2020
On 16 Jun, 2020
Received 10 Jun, 2020
Received 05 Jun, 2020
On 02 Jun, 2020
On 02 Jun, 2020
Invitations sent on 01 Jun, 2020
On 16 May, 2020
On 15 May, 2020
On 15 May, 2020
On 24 Apr, 2020
Received 22 Apr, 2020
On 19 Apr, 2020
Received 19 Apr, 2020
On 14 Apr, 2020
Invitations sent on 14 Apr, 2020
On 14 Apr, 2020
On 13 Apr, 2020
On 13 Apr, 2020
On 10 Apr, 2020
Qualitative and quantitative assay of glucose 6 phosphate dehydrogenase in patients attending tertiary care center
Posted 26 May, 2020
On 22 Jun, 2020
On 16 Jun, 2020
Received 10 Jun, 2020
Received 05 Jun, 2020
On 02 Jun, 2020
On 02 Jun, 2020
Invitations sent on 01 Jun, 2020
On 16 May, 2020
On 15 May, 2020
On 15 May, 2020
On 24 Apr, 2020
Received 22 Apr, 2020
On 19 Apr, 2020
Received 19 Apr, 2020
On 14 Apr, 2020
Invitations sent on 14 Apr, 2020
On 14 Apr, 2020
On 13 Apr, 2020
On 13 Apr, 2020
On 10 Apr, 2020
Objectives: The study was carried out with the aim to find out the frequency of Glucose 6 phosphate dehydrogenase (G6PD) deficiency among the patients attending the hospital and to rationalize the qualitative methemoglobin reduction test in reference to the quantitative spectrophotometric assay. Timely screening of the patients for G6PD with appropriate screening method can play an important role in preventing hemolytic crisis that arises from therapeutic use of oxidative drugs like primaquine.
Result: The frequency of G6PD deficient cases was 3% by both of the employed tests. The mean ± SD of G6PD activity in the patients under study was 15.34 ± 4.7 IU/g Hb in males and 16.01 ± 3.74 IU/g Hb in females. G6PD activity was positively associated with reticulocyte count (r = 0.289, p-value = 0.004) and negatively with mean corpuscular hemoglobin concentration (r = -0.220, p-value = 0.028). The correlation of red blood corpuscular count and G6PD was statistically significant (p-value = 0.048).