In the current study a drug delivering bilayer porous/nanofibrous wound dressing was developed using a combination of electrospinning and freeze-drying methods. The wound dressings were prepared by lyophilization of 1:1 weight ratios of calcium alginate and carboxymethyl cellulose (CMC) solutions. Drug delivering nanofibrous sheets were fabricated by electrospinning of polyvinyl alcohol (PVA) solution incorporated with 1%,3%,5%, and 10% of Propionyl-L-carnitine. The dressings were studied regarding their microstructure, swelling capacity, mechanical strength, surface wettability, water vapor permeability, drug release profile, in vitro degradation, cell viability assay, hemocompatibility, porosity measurement, microbial penetration assay, and protein adsorption assay. Based on in vitro studies, PVA sheets loaded with 5% Propionyl-L-carnitine was chosen for the preparation of wound dressings. The healing potential of the produced constructs was studied in rat model of diabetic wound. Our results showed that the drug delivering dressings demonstrated significantly higher wound closure and better histological regeneration compared to drug free constructs and sterile gauze. Our results suggest potential applicability of Propionyl-L-carnitine delivering Calcium Alginate/CMC/PVA dressing for the treatment of diabetic wounds in clinic.