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Research Article
Hongxu Sun
jiangnan university
Corresponding Author
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Sleep disturbance is common in patients with Alzheimer’s disease (AD), and orexin A is a pivotal neurotransmitter for bidirectional regulating the amyloid-β (Aβ) deposition of AD brain and poor sleep. In the present study, we examined the characteristic of sleep-wake architecture in APPswe/PSldE9 (APP/PS1) and Aβ-treated mice using electroencephalogram (EEG) and electromyographic (EMG) analysis. We compared the expression of orexin A, distribution, and morphology of the corresponding orexin A neurons using innovative methods including three-dimensional reconstruction and brain tissue clearing between Wild type (WT) and APP/PS1 mice. Results from our study demonstrated that increased wakefulness and reduced NREM sleep were seen in APP/PS1 and Aβ treated mice while the expression of orexin A was significantly upregulated. Higher density and distribution of orexin A activated neurons were seen in APP/PS1 mice, with a location of 1.06 mm to 2.30 mm away from the anterior fontanelle compared to 1.34 mm to 2.18 mm away from the anterior fontanelle in WT mice. These results suggested that the population and distribution of orexin A may play an important role in the progression of AD.
Keywords
Alzheimer’s disease, sleep, three-dimensional reconstruction, orexin A neurons, amyloid-β
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CURRENT STATUS: Under Review
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Posted 07 Jun, 2021
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Received 06 Jun, 2021
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This preprint is under consideration at Brain Structure and Function. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Hongxu Sun
jiangnan university
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 07 Jun, 2021
No community comments so far
Reviews received
Received 06 Jun, 2021
Reviewers invited
Invitations sent on 05 Jun, 2021
Editor invited
On 04 Jun, 2021
First submitted
On 03 Jun, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Sleep disturbance is common in patients with Alzheimer’s disease (AD), and orexin A is a pivotal neurotransmitter for bidirectional regulating the amyloid-β (Aβ) deposition of AD brain and poor sleep. In the present study, we examined the characteristic of sleep-wake architecture in APPswe/PSldE9 (APP/PS1) and Aβ-treated mice using electroencephalogram (EEG) and electromyographic (EMG) analysis. We compared the expression of orexin A, distribution, and morphology of the corresponding orexin A neurons using innovative methods including three-dimensional reconstruction and brain tissue clearing between Wild type (WT) and APP/PS1 mice. Results from our study demonstrated that increased wakefulness and reduced NREM sleep were seen in APP/PS1 and Aβ treated mice while the expression of orexin A was significantly upregulated. Higher density and distribution of orexin A activated neurons were seen in APP/PS1 mice, with a location of 1.06 mm to 2.30 mm away from the anterior fontanelle compared to 1.34 mm to 2.18 mm away from the anterior fontanelle in WT mice. These results suggested that the population and distribution of orexin A may play an important role in the progression of AD.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
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