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Research
Yanfeng Hu
Department of general surgery, Nanfang Hospital, Southern Medical University, Guangzhou
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5608-3587
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Paclitaxel (PTX) has been suggested to be a promising front-line drug for gastric cancer (GC), while P-glycoprotein (P-gp) could lead to drug resistance by pumping PTX out of GC cells. Consequently, it might be a hopeful way to combat drug resistance by inhibiting the out-pumping function of P-gp.
Results
In this study, we developed a drug delivery system incorporating PTX onto polyethylene glycol (PEG)-modified and oxidized sodium alginate (OSA) -functionalized graphene oxide (GO) nanosheets (NSs), called [email protected] Owing to pH/thermal-sensitive drug release properties, [email protected] could induced more obvious antitumor effects on GC, compared to free PTX. With near infrared (NIR)-irradiation, [email protected] could generate excessive reactive oxygen species (ROS), attack mitochondrial respiratory chain complex enzyme, reduce adenosine-triphosphate (ATP) supplement for P-gp, and effectively inhibit P-gp’s efflux pump function. Since that, [email protected] achieved better therapeutic effect on PTX-resistant GC without evident toxicity (Scheme 1).
Conclusions
In conclusion, [email protected] could serve as a desirable strategy to reverse PTX’s resistance, combined with chemo/photothermal/photodynamic therapy.
Keywords
Graphene oxide (GO), drug resistance, P-glycoprotein (P-gp), chemo/photothermal (PTT)/ photodynamic (PDT) therapy, mitochondrial respiratory chain
Figure 1
Figure 2
Figure 3
Figure 4
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Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
- scheme1.jpg
The mechanism of [email protected] NSs during the treatment of PTX-resistant GC cells.
- scheme2.jpg
Schematic for the preparation of [email protected] NSs
- Supplementarymaterials.doc
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CURRENT STATUS: Under Review
Version 1
Posted 22 Feb, 2021
No community comments so far
Editor invited
On 01 Dec, 2021
Editor assigned
On 01 Dec, 2021
Editorial decision: Major revision
On 03 Apr, 2021
Review #2 received
Received 18 Mar, 2021
Reviewer #2 agreed
On 06 Mar, 2021
Review #1 received
Received 04 Mar, 2021
Reviews received
Received 21 Feb, 2021
Reviewers invited
Invitations sent on 21 Feb, 2021
Reviewer #1 agreed
On 20 Feb, 2021
Submission checks complete
On 11 Feb, 2021
First submitted
On 07 Feb, 2021
Metrics
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Subject Areas
Nanoscience
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This preprint is under consideration at Journal of Nanobiotechnology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Yanfeng Hu
Department of general surgery, Nanfang Hospital, Southern Medical University, Guangzhou
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5608-3587
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 22 Feb, 2021
No community comments so far
Editor invited
On 01 Dec, 2021
Editor assigned
On 01 Dec, 2021
Editorial decision: Major revision
On 03 Apr, 2021
Review #2 received
Received 18 Mar, 2021
Reviewer #2 agreed
On 06 Mar, 2021
Review #1 received
Received 04 Mar, 2021
Reviews received
Received 21 Feb, 2021
Reviewers invited
Invitations sent on 21 Feb, 2021
Reviewer #1 agreed
On 20 Feb, 2021
Submission checks complete
On 11 Feb, 2021
First submitted
On 07 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Nanoscience
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Paclitaxel (PTX) has been suggested to be a promising front-line drug for gastric cancer (GC), while P-glycoprotein (P-gp) could lead to drug resistance by pumping PTX out of GC cells. Consequently, it might be a hopeful way to combat drug resistance by inhibiting the out-pumping function of P-gp.
Results
In this study, we developed a drug delivery system incorporating PTX onto polyethylene glycol (PEG)-modified and oxidized sodium alginate (OSA) -functionalized graphene oxide (GO) nanosheets (NSs), called [email protected] Owing to pH/thermal-sensitive drug release properties, [email protected] could induced more obvious antitumor effects on GC, compared to free PTX. With near infrared (NIR)-irradiation, [email protected] could generate excessive reactive oxygen species (ROS), attack mitochondrial respiratory chain complex enzyme, reduce adenosine-triphosphate (ATP) supplement for P-gp, and effectively inhibit P-gp’s efflux pump function. Since that, [email protected] achieved better therapeutic effect on PTX-resistant GC without evident toxicity (Scheme 1).
Conclusions
In conclusion, [email protected] could serve as a desirable strategy to reverse PTX’s resistance, combined with chemo/photothermal/photodynamic therapy.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
- scheme1.jpg
The mechanism of [email protected] NSs during the treatment of PTX-resistant GC cells.
- scheme2.jpg
Schematic for the preparation of [email protected] NSs
- Supplementarymaterials.doc
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