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Research Article
Renu Pandey
Indian Agricultural Research Institute
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9244-8579
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Aims The mobility of phosphorus (P) depends on availability of water in soil; both are limited resources for crop production. We studied the mechanisms governing cross tolerance in the contrasting mungbean accessions for drought and low P stress.
Methods Tolerant (IC333090 and IC507340) and sensitive (IC488526 and EC397142) mungbean accessions were grown in soil with treatments: control (sufficient P, irrigated), low P (no P, irrigated), drought (sufficient P, irrigation withheld), and combined stress (no P, irrigation withheld) as well as recovery.
Results Drought reduced the relative water content and membrane stability index, affecting overall plant growth. Combined stress (low P and drought) significantly increased root growth, leaf area, and biomass in tolerant accessions, which was attributed to enhanced nutrient uptake and symbiotic N2-fixation. Combined stress also increased osmolyte concentration, antioxidative compounds, and scavenging activity of antioxidant enzymes in tolerant accessions while recovery from drought significantly reduced osmolyte concentration. Transcript abundance of candidate genes related to drought and low P was significantly higher in leaves of IC333090 than IC488526. Conversely, low-P-induced genes (VrSPX1, VrPHO1, VrSQD1, VrPEPCase, and VrMDH) in IC488526 were either downregulated or did not significantly change under combined stress. The drought recovery was better in IC333090 due to enhanced expression of stress-responsive genes.
Conclusions Tolerant mungbean accession could be used as potential donor parents in breeding programs. Traits imparting cross tolerance to drought and low P stress may facilitate better varietal selection for increased crop productivity under low P, drought, and the combined stress.
Keywords
Drought, Low phosphorus, Osmolytes, Oxidative metabolism, Biological N2-fixation, Relative gene expression, Vigna radiata
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CURRENT STATUS: Under Review
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Posted 22 Feb, 2021
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Reviews received
Received 22 Feb, 2021
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This preprint is under consideration at Plant and Soil. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Renu Pandey
Indian Agricultural Research Institute
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9244-8579
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 22 Feb, 2021
No community comments so far
Reviews received
Received 22 Feb, 2021
Reviewers invited
Invitations sent on 19 Feb, 2021
Editor invited
On 09 Feb, 2021
Editor assigned
On 09 Feb, 2021
First submitted
On 08 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Aims The mobility of phosphorus (P) depends on availability of water in soil; both are limited resources for crop production. We studied the mechanisms governing cross tolerance in the contrasting mungbean accessions for drought and low P stress.
Methods Tolerant (IC333090 and IC507340) and sensitive (IC488526 and EC397142) mungbean accessions were grown in soil with treatments: control (sufficient P, irrigated), low P (no P, irrigated), drought (sufficient P, irrigation withheld), and combined stress (no P, irrigation withheld) as well as recovery.
Results Drought reduced the relative water content and membrane stability index, affecting overall plant growth. Combined stress (low P and drought) significantly increased root growth, leaf area, and biomass in tolerant accessions, which was attributed to enhanced nutrient uptake and symbiotic N2-fixation. Combined stress also increased osmolyte concentration, antioxidative compounds, and scavenging activity of antioxidant enzymes in tolerant accessions while recovery from drought significantly reduced osmolyte concentration. Transcript abundance of candidate genes related to drought and low P was significantly higher in leaves of IC333090 than IC488526. Conversely, low-P-induced genes (VrSPX1, VrPHO1, VrSQD1, VrPEPCase, and VrMDH) in IC488526 were either downregulated or did not significantly change under combined stress. The drought recovery was better in IC333090 due to enhanced expression of stress-responsive genes.
Conclusions Tolerant mungbean accession could be used as potential donor parents in breeding programs. Traits imparting cross tolerance to drought and low P stress may facilitate better varietal selection for increased crop productivity under low P, drought, and the combined stress.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
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