Currently, several pathogens, including enteric viruses, which affect humans and the most varied species of animals have been registered as responsible for 60% of zoonotic infections in humans. Zoonoses are responsible for 75% of the emerging and reemerging diseases that affect humans and cause at least 20% of losses in animal production , where the wild environment has also been responsible for most zoonotic infections .
In the present study, PAGE was one of the techniques used to detect PBV, with the aim of visualizing the profile of electrophoretic migration of genomic segments. However, this test is characterized by having a high specificity and low sensitivity. Then, suggesting that the negative results, obtained from fecal samples from animals, have a correlation with low viral excretion and the low sensitive by PAGE technique. However, such samples may show positivity when analyzed by RT-PCR, since this technique has greater sensitivity and specificity , in addition to the detection of viral nucleic acid may occur even though fecal samples do not show high viral titers .
Despite the low sensitivity of PAGE, several epidemiological studies use this technique in the screening stage of diarrheal infections due the electrophoretic profile of these viruses, which helps to outline the scenario of infections caused by these agents and its reach zone in the world    .
Clinical aspect of animals and fecal specimens were not considered, since detection may happen even though hosts, in many cases, remain asymptomatic [4, 8]. However, this fact could have influenced on PAGE results, suggesting the quantity of viral particles eliminated in fecal excrement was not sufficient for detection by the test.
Previous studies carried out with fecal samples of broilers (Gallus gallus), from northeastern region of the state of Pará, reported positivity for PBV, by PAGE from 15.3%  to 30% . The same protocols were used to perform the PAGE technique, although divergent results when compared to this study, believing that the reduced viral load excreted was the interferer to positivity.
PBV are reported in some different hosts, 3.63% in cats from Portugal , 14.28% in horses , 11.15% in pigs , and 0.73% in cattle in India . In Brazil, the positivity in swine is concentrated from 12.45%  to 43.24% , 8.30% in cattle , 0.86 % to 1.84%  in canines, and 3.4%  to 49.4%  in broilers. There have no positivity in rodents of the didelfideos family and in wild birds. Therefore, the present study corroborates the previously reported data, considering that the population with the highest frequency of PBVs (28.13%) was in swine.
The cities of samples came from in this study showed high rates of deforestation during 2014 to 2016, due the use of the land for agricultural and livestock production, where families residing in these areas use these activities as a source of subsistence . It becomes evident that the increase in the area of fragmentation, the alteration of the habitat and ecological niches of animals caused by anthropic pressure, and consequently the closer contact between humans and wild animals, contribute to the occurrence of infectious diseases, spread of emerging pathogens and new hosts [43–45]. This fact may have contributed to the fact that the percentage of positivity among the studied species occurred due to the wide interaction between the species, facilitated by the change in the environment where they live.
The dissemination of PBV among the most varied animal hosts in the present study could be confirmed by phylogenetic analysis. The virus presence in canines, felines, cattle, poultry, swine, and in humans, shows the lack of host specificity, corroborating what has already been demonstrated in studies with swine [46, 47].
Phylogenetic analysis reported the virus circulated in several species of animals, regardless of time and geographic area of detection, as previously reported, suggesting a potential of spreading that pathogen presents .
Rodents, birds and bats are among the wild animals in this study. Considering a fecal-oral infection route, and that the diet of rodents (Marmosa sp, family Didelphidae) is composed of small vertebrates (baby birds, chickens), fruits, decomposing organic matter (carrion) even invertebrates , suggesting that the feeding habits of these animals may have contributed to their infection by ingesting nutrients exposed in the contaminated environment.
Chiropterans, except Desmodus rotundus species (blood-sucking habit), the other species: Carollia perspicillata and Phyllostomus hastatus are frugivorous . Characteristics such as diet, ability to fly, seasonal migration, behavioral patterns, affinity to live in colonies, and other, including this group as a great zoonotic potential host . However, there was no positivity for this order (Chiroptera) in this study
Birds, and chiropterans, are of great importance for public health and are considered as potential reservoirs of pathogens that cause zoonotic diseases and due to their ability to fly, they can act in the dispersion of viruses and thus establish new foci of emerging diseases or reemerging along their paths [28, 51].
Referring to pets (canines and felines) and livestock animals (cattle, horses, pigs and birds) due they share the same space and environment, circulate in forest and home area, and interact with each other and with humans, the chances of viral transmission are high, especially in cases of animals infected in a phase of viral excretion. Therefore, the strong anthropic pressure in Amazon has been an important factor of spread zoonotic diseases, infecting new hosts, and expanding pathogens with wide-reaching potential through the interaction between species, including enteric pathogens such as PBV and RV .
In conclusion, in this study the incidence of PBV GG-I occurred in all groups of animals, except in bats. This study is a pioneer in characterizing PBV, G-I, in felines (Felis catus) in Brazil. These data suggest the possible interspecies transmission of PBV among the animals included in this study, considering that some animals were grouped with different taxon and some samples formed clusters with strains similar to strains isolated in porcine detected mainly in China.