DOI: https://doi.org/10.21203/rs.3.rs-2334072/v1
Recently, several studies investigated the association between lactate dehydrogenase (LDH) level and the prognosis of urothelial carcinoma. However, no studies explored the role of serum LDH level in the survival of overall bladder cancer (BC). In this study, we intended to address the association of LDH level with the prognosis of BC.
206 patients with BC were included in this study. The clinical data and blood samples of patients were collected. The overall survival (OS) and progression-free survival (PFS) were used. Kaplan-Meier method and Log rank test were used to evaluate the effects of LDH level on the survival of BC. Univariate and multivariate Cox regression analyses were utilized to identify prognosis predictors of BC.
Data indicated that serum LDH level in the BC patients was significantly higher than those in controls. In addition, this study suggested that serum LDH level was associated with T stage, N stage, tumor size, M stage, pathological type, treatment methods, and lymphovascular invasion. The Kaplan-Meier analysis found significant differences in the OS and PFS rate between lower and higher serum LDH level groups (LDH ≥ 225 U/L and < 225 U/L). Multivariate Cox regression indicated that pathological type, treatment methods, and higher level of LDH were independently associated with adverse prognosis in BC patients.
The higher serum LDH level (≥ 225 U/L) is associated with poor prognosis in patients with BC. Serum LDH level could be used as a novel predictive biomarker for BC patients.
Bladder cancer (BC) is a prevalent malignancy in women and is the fourth most main malignancy in men [1]. More than 430,000 BC patients were diagnosed globally every year, which brings a heavy burden for the society [2]. A host of risk factors including cigarette smoking, alcohol intake, diets low in fruits and vegetables, urban living, occupational exposure to carcinogens, and genetic factors were associated with the development of BC [3]. Among these factors, cigarette smoking is the most primary risk factor for BC. It is estimated that tobacco was responsible for approximately 50% of all BC patients [4]. The most common symptom of BC is haematuria [5]. However, some BC patients with microscopic haematuria were not adequate [6]; and there were no effective screening methods for BC, except for the cystoscope. Thus, detecting novel prognostic and diagnostic biomarkers is urgently required for patients with BC.
Most cancer cells utilize glucose metabolism by glycolysis to produce enough ATP to promote the development of cancer, which is served as the Warburg effect [7]. Among the enzymes participating in glycolysis, lactate dehydrogenase (LDH), an oxidoreductase, could converts pyruvate to lactate at the end of glycolysis; thereby, LDH is regarded as a promising molecular target for the development of new glycolytic inhibitors used in cancer therapy [8]. Serum LDH level may be a biomarker of tumor development due to its association with tumor metabolic changes [9].
Urothelial carcinoma (UC) mainly consists of urothelial carcinoma of the bladder (UCB) and upper tract urothelial carcinoma (UTUC). Recently, a host of studies investigated the link between LDH level and the prognosis of UC.[10–24] Among these studies, no studies investigated the role of serum LDH level in the survival of overall BC; and previous studies mainly focused on UCB patients (the main type of BC). In this study, we intended to address the association of LDH level with clinicopathological markers of BC. In addition, we aimed to evaluate the ability of pretreatment serum LDH level in predicting the prognosis of BC patients.
This study was conducted after approval from the Ethics Committee of Huaian No.1 People’s Hospital. 206 consecutive BC patients were recruited between September 2019 and May 2017 from our Hospital. BC patients were divided into two groups, which was according to the pretherapeutic median levels of LDH. The diagnosis of BC patients was according to histological results. All relevant clinical and pathologic information was collected for each patient. Inclusion criteria for BC patients were as follows: one, patients with definite histologic evidence; two, patients older than 18 years; three, patients with complete data. Patients with other cancers, hepatitis, heart disease, and hemopathy were excluded. The informed consent form was provided by all subjects. This study was approved by the Ethics Committee of this hospital. This study was in consistent with the Declaration of Helsinki.
Clinical information was collected from the medical records, including age, sex (male and female), smoking status (yes and no), T stage, N stage (N0 and N1-N3), tumor size (< 3 cm and > 3 cm), M stage (M0 and M1), lymphovascular invasion (LVI) status, perineural invasion (PNI) status, pathological type, treatment methods, and multifocality (Unifocal and Multifocal). Serum LDH levels were collected from all BC patients before treatment. Overall survival (OS) was defined as the survival duration from the date of initial diagnosis to death due to any reasons or last follow-up. Progression-free survival (PFS) referred to the time for BC patients to survive without progression or death after treatment. The follow-up strategies were as follows: every two months for the first 2 years, quarterly for the next 2 years, and then semiannually for the remaining time thereafter. The follow-up time ended in June 2021.
The correlation between serum LDH level and clinicopathological indexes was analyzed. We used the receiver operating characteristic (ROC) curve to assess the diagnostic ability of serum LDH level for detecting BC. The log-rank test was used to compare the OS and PFS between different groups. Univariate and Multivariate Cox regression analyses were conducted to identify prognostic variables associated with the survival of BC. Risk factors with a P value < 0.05 in univariate analysis were choose in further multivariate Cox regression analysis. 95% confidence interval (CI) and Hazard risk (HR) were figured out. Statistically significant Differences were set at P < 0.05. SPSS (version 19.0, Chicago, IL, USA), and Graphpad Prism (version 7.0, La Jolla, CA, USA), and MedCalc were used for statistical analyses.
The cut-off value of LDH level was defined as 225 U/L, which was the upper limit of normal range in this hospital. Thus, we defined the high LDH level group was over 225 U/L, and the low LDH level group was lower than 225 U/L. Clinicopathological variables for BC patients with LDH ≥ 225 U/L and < 225 U/L are shown in Table 1. 223 patients with BC were evaluated initially, and 206 cases were included finally (Fig. 1). Among the 206 BC patients, 149 cases were in the low LDH group and 57 cases were in the high LDH group. We found that LDH level was associated with T stage, N stage, tumor size, M stage, pathological type, treatment methods, and LVI status. No significant differences were observed in two groups regarding age, sex, smoking status, PNI status, and multifocality status.
Variables | Total patients | Groups | P | ||
---|---|---|---|---|---|
High LDH | Low LDH | ||||
(n = 206) | < 225 U/L | ≥ 225 U/L | |||
Median age (years) | 0.073 | ||||
< 64 | 93 | 73 | 20 | ||
≥ 64 | 113 | 76 | 37 | ||
Sex, n% | 0.709 | ||||
Male | 159 | 114 | 45 | ||
Female | 47 | 35 | 12 | ||
Smoking | 0.592 | ||||
Yes | 95 | 67 | 28 | ||
No | 111 | 82 | 29 | ||
Pathological type | < 0.001 | ||||
Transitional cell carcinoma | 188 | 144 | 44 | ||
Squamous cell carcinoma | 12 | 4 | 8 | ||
Adenocarcinoma | 6 | 1 | 5 | ||
T stage | < 0.001 | ||||
Ta, Tis, T1 | 122 | 101 | 21 | ||
T2-3 | 84 | 48 | 36 | ||
N stage | < 0.001 | ||||
N0 | 183 | 144 | 39 | ||
N1-N3 | 23 | 5 | 18 | ||
M stage | < 0.001 | ||||
M0 | 188 | 146 | 42 | ||
M1 | 18 | 3 | 15 | ||
Tumor size (cm) | 0.011 | ||||
< 3 cm | 55 | 47 | 8 | ||
> 3 cm | 151 | 102 | 49 | ||
LVI | < 0.001 | ||||
Present | 76 | 43 | 33 | ||
Absent | 130 | 106 | 24 | ||
PNI | 0.075 | ||||
Present | 29 | 17 | 12 | ||
Absent | 177 | 132 | 45 | ||
Multifocality | 0.559 | ||||
Unifocal | 161 | 118 | 43 | ||
Multifocal | 45 | 31 | 14 | ||
Treatment methods | < 0.001 | ||||
Surgery | 194 | 147 | 47 | ||
Chemotherapy | 9 | 2 | 7 | ||
Immunotherapy | 3 | 0 | 3 | ||
LVI, lymphovascular invasion; PNI, perineural invasion, LDH, Lactate dehydrogenase. | |||||
Bold values are statistically significant (P < 0.05). |
230 healthy controls were included in this study. The detailed information for controls is summarized in Supplementary Table 1. We measured the serum level of LDH in BC patients and controls, and found that the LDH level in BC group were significantly higher than those in control group. We used the ROC curve to evaluate the diagnostic function of serum LDH level for BC (Fig. 2). The sensitivity and specificity were 47.57% and 71.74% with a cutoff value of 211.53 (Supplementary Table 2), respectively. The area under the curve was 0.615 (95%CI: 0.567–0.661, P < 0.001), suggesting that the diagnostic ability of serum LDH level for detecting BC was relatively moderate.
We divided all BC patients into two groups (LDH ≥ 225 U/L and < 225 U/L) according to the level of LDH. In order to evaluate the prognostic value of LDH in BC, Kaplan-Meier analysis was utilized to assess the association between LDH level and follow-up data, and the log-rank test was mainly used for analyzing statistical difference. The results showed that the high level of LDH was negatively correlated with OS and PFS (Figs. 3 and 4), suggesting that the survival rate of BC patients with lower level of LDH were significantly higher than those with higher level of LDH. In addition, we compared the survival rates between UCB group and non-UCB group (squamous cell carcinoma and adenocarcinoma). Data indicated that the survival rate of BC patients with UCB was significantly higher than that in non-UCB group (Supplementary Figs. 1 and 2).
Univariate and multivariate Cox analyses were used to identify prognostic factors of BC patients. Univariate analysis showed that older age, T2-3 stage, N1-3 stage, M1 stage, LVI, PNI, non-UCB, treatment methods (chemotherapy/immunotherapy vs. surgery), and higher level of LDH were significantly associated with reduced OS (Table 2), while smoking, T2-3 stage, N1-3 stage, M1 stage, tumor size ≥ 3cm, LVI, PNI, non-UCB, treatment methods (chemotherapy/immunotherapy vs. surgery), and higher level of LDH negatively correlated with PFS (Table 3). Multivariate analysis indicated that T2-3 stage, non-UCB, treatment methods (chemotherapy/immunotherapy vs. surgery), and higher level of LDH were independent predictive factors for worse OS, while tumor size ≥ 3cm, non-UCB, treatment methods (chemotherapy/immunotherapy vs. surgery), and higher level of LDH correlated with adverse PFS. In addition, we identified prognostic factors for BC patients with UCB, and found that older age, T2-3 stage, treatment methods (chemotherapy/immunotherapy vs. surgery), and higher LDH level were prognostic factors for worse OS (Supplementary Table 3). Furthermore, we observed that T2-3 stage, M1 stage, Tumor size ≥ 3cm were adverse factors for PFS in UCB (Supplementary Table 4).
Factors | Overall survival | |||
---|---|---|---|---|
Univariate | Multivariate | |||
HR (95%CI) | P-value | HR (95%CI) | P-value | |
Age | ||||
≥ 64 vs. < 64 years | 3.48(1.69–7.21) | 0.001 | 1.43(0.64–3.19) | 0.384 |
Sex | ||||
Male vs. female | 0.88(0.46–1.70) | 0.713 | - | - |
Smoking | ||||
Yes vs. no | 1.43(0.80–2.54) | 0.226 | - | - |
Pathological type | ||||
Squamous/Adeno- vs. Transitional cell carcinoma | 16.91(8.69–32.90) | < 0.001 | 3.18(1.28–7.88) | 0.013 |
T stage | ||||
T2-T3 vs. Ta, Tis, T1 | 8.02(3.75–17.18) | < 0.001 | 3.43(1.44–8.14) | 0.005 |
N stage | ||||
N1-N3 vs. N0 | 13.92(7.65–25.32) | < 0.001 | 1.95(0.48–7.82) | 0.348 |
M stage | ||||
M1 vs. M0 | 14.77(7.94–27.46) | < 0.001 | 0.88(0.26–3.03) | 0.839 |
Tumor size | ||||
≥ 3 vs. < 3cm | 1.64(0.79–3.40) | 0.182 | - | - |
LVI | ||||
Present vs. absent | 4.74(2.54–8.87) | < 0.001 | 1.47(0.65–3.36) | 0.358 |
PNI | ||||
Present vs. absent | 3.04(1.60–5.76) | 0.001 | 1.05(0.43–2.55) | 0.918 |
Multifocality | ||||
Multifocal vs. unifocal | 1.04(0.53–2.05) | 0.900 | - | - |
Treatment methods | ||||
Chemotherapy/Immunotherapy vs. Surgery | 18.20(8.78–37.74) | < 0.001 | 2.76(1.06–7.18) | 0.037 |
LDH | ||||
High vs. low | 7.23(3.94–13.24) | < 0.001 | 3.47(1.72–6.96) | < 0.001 |
LVI, lymphovascular invasion; PNI, perineural invasion, LDH, Lactate dehydrogenase. | ||||
Bold values are statistically significant (P < 0.05). |
Factors | Progression-free survival | ||||
---|---|---|---|---|---|
Univariate | Multivariate | ||||
HR (95%CI) | P-value | HR (95%CI) | P-value | ||
Age | |||||
≥ 64 vs. < 64 years | 1.03(0.66–1.60) | 0.910 | - | - | |
Sex | |||||
Male vs. female | 1.52(0.84–2.76) | 0.165 | - | - | |
Smoking | |||||
Yes vs. no | 1.59(1.02–2.48) | 0.041 | 1.49(0.93–2.37) | 0.097 | |
Pathological type | |||||
Squamous/Adeno- vs. Transitional cell carcinoma | 9.12(4.76–17.49) | < 0.001 | 2.60(1.12–6.03) | 0.026 | |
T stage | |||||
T2-T3 vs. Ta, Tis, T1 | 2.69(1.72–4.22) | < 0.001 | 1.68(0.99–2.85) | 0.055 | |
N stage | |||||
N1-N3 vs. N0 | 13.67(7.58–24.65) | < 0.001 | 3.00(0.80-11.35) | 0.105 | |
M stage | |||||
M1 vs. M0 | 18.37(9.42–35.84) | < 0.001 | 2.05(0.54–7.68) | 0.289 | |
Tumor size | |||||
≥ 3 vs. < 3cm | 3.20(1.60–6.42) | 0.001 | 2.59(1.26–5.35) | 0.010 | |
LVI | |||||
Present vs. absent | 1.82(1.17–2.83) | 0.008 | 0.82(0.45–1.50) | 0.523 | |
PNI | |||||
Present vs. absent | 2.18(1.22–3.91) | 0.008 | 1.18(0.54–2.60) | 0.672 | |
Multifocality | |||||
Multifocal vs. unifocal | 1.01(0.60–1.71) | 0.978 | - | - | |
Treatment methods | |||||
Chemotherapy/Immunotherapy vs. Surgery | 19.06(8.96–40.55) | < 0.001 | 2.80(1.02–7.70) | 0.046 | |
LDH | |||||
High vs. low | 3.08(1.96–4.83) | < 0.001 | 1.74(1.03–2.94) | 0.037 | |
LVI, lymphovascular invasion; PNI, perineural invasion, LDH, Lactate dehydrogenase. | |||||
Bold values are statistically significant (P < 0.05). |
This study suggested that serum LDH level was associated with T stage, N stage, tumor size, M stage, pathological type, treatment methods, and LVI status. Serum LDH level had a moderate diagnostic value in detecting BC patients. Multivariate Cox regression indicated that non-UCB, treatment methods, and higher level of LDH were independently associated with adverse prognosis in BC patients (including OS and PFS). Therefore, this study provided evidence that higher LDH level was associated with worse prognosis in BC and could be a prognostic indicator of BC patients.
Hannisdal et al. from Denmark first showed that UCB patients with LDH > 400 U/L showed shorter survival comparing to those with LDH < 400 U/L [11]. Two Japanese studies found that higher LDH level was negatively associated with the OS of UCB after radical cystectomy [12] or postcystectomy recurrent UCB [13]. However, a study by Yang et al. from Taiwan indicated that serum LDH level was not related with the disease-specific survival of UCB in their population [10], which was not consistent with previous findings [11–13]. We assumed the following factors may provide evidence for these conflict points. One, the results of Taiwanese study was obtained by the univariate analysis [10], which may underpower the credibility of results. Two, clinical heterogeneity should not be ignored. Two Japanese studies investigated the recurrent UCB [12, 13], while the Taiwanese study explored invasive UCB [10]. Three, their indicators evaluating survival differed, such as OS or disease-free survival (DFS). Four, their varied treatment therapies may also contribute to their conflicting results concerning the survival. It is of note that Yang et al. conducted another study [14], and found that serum LDH level ranging from 200 to 300 U/L was an independent factor associated with UTUC after multivariate analysis, but not for UCB. Obviously, the tumor location may be a crucial reason for explaining their contradictory results by Yang et al. [10, 14] Last but not least, the cut-off values of serum LDH level were diverse, which may exert effects on the final result.
Regarding UTUC, other studies [15–17] also delineated the association between serum LDH level and the prognosis of UTUC. Zhang et al. showed that preoperative serum LDH level was as a negative predictor of OS and DFS [15], while Tan et al. indicated that preoperative LDH was not an independent prognostic indicator for patients with UTUC [15]. However, Tan et al. suggested that elevated LDH level correlated with worse OS in UTUC patients with localized disease [16]. Kluth et al. also indicated that serum LDH level was not associated with the survival of UTUC [18]. As for the Japanese study by Ito et al., they observed that LDH ≥ 210 IU/L were significantly related with extraurothelial recurrence in N0M0 patients with renal pelvic cancer [17], but not OS or DFS.
Besides UCB and UTUC, some researchers shed light on the investigation of serum LDH level and UC survival. Sengelov et al. first showed that LDH level were related significantly to the survival of UC by univariate analyses [19], similar to the findings by Japanese studies via multivariate analyses [20–22]. In addition, two studies from Japan [23] and Spain [24] indicated that serum LDH level was not a prognostic factor in UC patients. We hypothesized that the negative results in the Spanish study [24] may due to its limited sample size (only 56 UC cases). Additionally, they utilized univariate analyses to obtain these results [24], which underpowered the reliability of their results.
Due to these conflicting findings concerning UCB, UTUC or UC, Wu et al. conducted a meta-analysis to address this issue [25]. They suggested that a high pretreatment serum LDH level was linked with an inferior OS, cancer-specific survival, and DFS in UC patients [25]. Subgroup analyses revealed that high serum LDH level was associated with a poor OS and DFS in UTUC, and a short OS in UCB [25]. It is noticeable that the meta-analysis by Wu et al. did not include a study [18], which was in line with inclusion criteria of this meta-analysis. Another meta-analysis by Zhang et al. also indicated that a high LDH level was associated with an adverse prognosis in many solid tumors [26].
In this study, we included 206 BC patients, and found that a higher pretreatment serum LDH level was associated with an unfavorable prognosis in BC patients. Abovementioned studies primarily investigated the survival of UCB patients (the main type of BC), while this study explored the prognosis of overall BC with UCB, squamous cell carcinoma, and adenocarcinoma. We compared the survival rates between UCB group and non-UCB group, and found that the survival rate of BC patients with UCB was significantly higher than that in non-UCB group, which was not investigated in other studies. Furthermore, multivariate analysis indicated that pathological type (non-UCB vs UCB) was an independent predictive factor for worse OS and PFS.
This study had several limitations. First, the sample size of this study was not large enough. Second, some confounding factors affecting the survival of BC may not investigated in this study, thereby exerting effects on our results. Third, the findings observed by this cohort study were only yielded in one single center; thus, multi-center studies are urgently needed to verify these findings. Fourth, UTUC patients were not investigated in this study. Fifth, diverse treatment strategies may affect the survival analysis, thereby interfering the effect of serum LDH level on the prognosis of BC patients.
Totally, this study observes that a higher pretreatment serum LDH level is associated with adverse prognosis in BC patients. In addition, serum LDH level may predict the occurrence of BC patients. Thus, LDH level could serve as a prognostic biomarker for BC patients.
Funding
This study did not receive any funding in any form.
Author contributions
Shuo Gu and Chao Yang: study design. Chao Yang: data collection. Shuo Gu and Chao Yang: manuscript writing. Shuo Gu and Chao Yang: critical revision of the manuscript. Shuo Gu supervision. All authors reviewed the manuscript.
Acknowledgements
Not applicable.
Ethics and consent to participate
This study was approved by the Ethics Committee of The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University. This study was in consistent with the Declaration of Helsinki. Informed consent was obtained from all subjects and/or their legal guardian.
Consent for publication
Not Applicable.
Availability of data and materials
All data generated or analyzed during this study are included in this published article [and its supplementary information files].
Competing interests
We have read and understood the BMC policy on declaration of interests and declare that there are no conflicts of interest.