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Research article
Effect of mesenchymal stem cells combined with chondroitin sulfate in an osteoarthritis in vitro model
VEGA VILLAR-SUÁREZ
Universidad de Leon
Corresponding Author
ORCiD: https://orcid.org/0000-0002-4374-7492
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This work is licensed under a CC BY 4.0 License. Read Full License
Introduction: Osteoarthritis (OA) is a degenerative joint disease which affects the whole joint structure. Many authors have focused on the factors responsible for the development of inflammatory processes involved in OA. Adipose tissue-derived mesenchymal stem cells (ASCs) represent a promising alternative of cell-based therapy strategy in the treatment of OA which could be combined with any other drug. Chondroitin sulfate plays a protective role in the joint based on the decrease of pro-inflammatory cytokines, thus having an important role in activating and inhibiting the metabolic pathways in chondrocytes.
Aims: The effectiveness of chondroitin sulfate and ASCs combined in an in vitro model of OA has been evaluated in this study.
Materials: Cytokines and factors which are involved in OA as well as specific cartilage gene expression after adding ASCs and chondroitin sulfate have been discussed in detail.
Results: Our results show a decrease in the expression of all genes related to the pro-inflammatory cytokines analysed. Although there was no increase in the expression of the specific genes of the cartilage matrix, such as collagen type II and aggrecan.
Conclusions: This study shows the effectiveness of association of ASCs and chondroitin sulfate for the treatment of OA.
Keywords
Mesenchymal stem cells, chondroitin sulfate, osteoarthritis
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This is a preprint. It has not completed peer review.
Research article
Effect of mesenchymal stem cells combined with chondroitin sulfate in an osteoarthritis in vitro model
VEGA VILLAR-SUÁREZ
Universidad de Leon
Corresponding Author
ORCiD: https://orcid.org/0000-0002-4374-7492
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 2
Posted 15 Jun, 2020
No community comments so far
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Molecular Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Introduction: Osteoarthritis (OA) is a degenerative joint disease which affects the whole joint structure. Many authors have focused on the factors responsible for the development of inflammatory processes involved in OA. Adipose tissue-derived mesenchymal stem cells (ASCs) represent a promising alternative of cell-based therapy strategy in the treatment of OA which could be combined with any other drug. Chondroitin sulfate plays a protective role in the joint based on the decrease of pro-inflammatory cytokines, thus having an important role in activating and inhibiting the metabolic pathways in chondrocytes.
Aims: The effectiveness of chondroitin sulfate and ASCs combined in an in vitro model of OA has been evaluated in this study.
Materials: Cytokines and factors which are involved in OA as well as specific cartilage gene expression after adding ASCs and chondroitin sulfate have been discussed in detail.
Results: Our results show a decrease in the expression of all genes related to the pro-inflammatory cytokines analysed. Although there was no increase in the expression of the specific genes of the cartilage matrix, such as collagen type II and aggrecan.
Conclusions: This study shows the effectiveness of association of ASCs and chondroitin sulfate for the treatment of OA.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7