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Background: Plant roots host a repertoire of bacteria and fungi, whose ecological interactions could improve their functions and plant performance. However, potential interactions and underlying mechanisms remain largely unknown in root-associated microbial communities at a continuous fine-scale.
Results: We analyzed microbial intra- and inter-domain network topologies, keystone taxa, and interaction-related genes across four compartments (non-rhizosphere, rhizosphere, episphere and endosphere) from a soil-mangrove root continuum, using amplicon and metagenome sequencing technologies. We found that both intra- and inter-domain networks displayed notable differences in the structure and topology across four compartments. Compared to three peripheral compartments, the endosphere was a distinctive compartment with more intensive interactions in bacterial-fungal network than in bacterial or fungal network, which could be related to three bacterial keystone taxa (Vibrio, Anaerolineae and Desulfarculaceae) detected in the endosphere as they are known to intensify inter-domain interactions with fungi and stimulate biofilm formation. Also, high abundances of genes involved in cell-cell communications by quorum sensing (rhlI, lasI, pqsH and lasR) and aerobic cobamide biosynthesis (cobG, cobF and cobA) were detected in the endosphere.
Conclusions: Our results reveal intensified inter-domain interactions of endophytes in the mangrove roots, creating a distinct micro-environment to promote a biofilm life-style.
Keywords
Network, Bacterial-fungal interaction, Endosphere, Quorum sensing, Cobamide
Figure 1
Figure 2
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Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.pdf
Additional file 1: Table. S1. as PDF Additional file 1: Table S1. KO (KEGG Orthology) numbers of quorum sensing and cobamide biosynthesis related genes (345k)
- Additionalfile2.pdf
Additional file 2: Table. S2. as PDF Additional file 2: Table S2. Taxonomy information and relative abundance of keystone taxa in bacterial-fungal interaction (BFI), bacterial and fungal networks across the non-rhizosphere (Non), rhizosphere (Rhi), episphere (Epi) and endosphere (Endo) compartments (318k)
- Additionalfile3.pdf
Additional file 3: Table. S3. as PDF Additional file 3: Table S3. Key network topological characteristics for bacterial, fungal and bacterial-fungal interaction (BFI) networks across the non-rhizosphere (Non), rhizosphere (Rhi), episphere (Epi) and endosphere (Endo) compartments (228k)
- Additionalfile4.pdf
Additional file 4: Fig. S1. as PDF Additional file 4: Fig. S1. Highly connected modules within bacterial-fungal interaction (BFI) networks across the non-rhizosphere (Non), rhizosphere (Rhi), episphere (Epi) and endosphere (Endo) compartments (309k)
- Additionalfile5.pdf
Additional file 5: Fig. S2. as PDF Additional file 5: Fig. S2. Topological features of keystone taxa in (a) bacterial, (b) fungal and (c) bacterial-fungal interaction (BFI) networks across the non-rhizosphere (Non), rhizosphere (Rhi), episphere (Epi) and endosphere (Endo) compartments (343k)
- Additionalfile6.pdf
Additional file 6: Fig. S3. as PDF Additional file 6: Fig. S3. Degrees of keystone taxa from bacterial and fungal phyla in bacterial-fungal interaction (BFI) networks (322k)
- Additionalfile7.pdf
Additional file 7: Fig. S4. as PDF Additional file 7: Fig. S4. Bacterial community compositions across the non-rhizosphere (Non), rhizosphere (Rhi), episphere (Epi) and endosphere (Endo) compartments (333k)
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
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Prescreen
This manuscript passed our Prescreen assessment
Complete author information
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CURRENT STATUS: Posted
Version 1
Posted 26 Feb, 2021
No community comments so far
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Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Plant roots host a repertoire of bacteria and fungi, whose ecological interactions could improve their functions and plant performance. However, potential interactions and underlying mechanisms remain largely unknown in root-associated microbial communities at a continuous fine-scale.
Results: We analyzed microbial intra- and inter-domain network topologies, keystone taxa, and interaction-related genes across four compartments (non-rhizosphere, rhizosphere, episphere and endosphere) from a soil-mangrove root continuum, using amplicon and metagenome sequencing technologies. We found that both intra- and inter-domain networks displayed notable differences in the structure and topology across four compartments. Compared to three peripheral compartments, the endosphere was a distinctive compartment with more intensive interactions in bacterial-fungal network than in bacterial or fungal network, which could be related to three bacterial keystone taxa (Vibrio, Anaerolineae and Desulfarculaceae) detected in the endosphere as they are known to intensify inter-domain interactions with fungi and stimulate biofilm formation. Also, high abundances of genes involved in cell-cell communications by quorum sensing (rhlI, lasI, pqsH and lasR) and aerobic cobamide biosynthesis (cobG, cobF and cobA) were detected in the endosphere.
Conclusions: Our results reveal intensified inter-domain interactions of endophytes in the mangrove roots, creating a distinct micro-environment to promote a biofilm life-style.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.pdf
Additional file 1: Table. S1. as PDF Additional file 1: Table S1. KO (KEGG Orthology) numbers of quorum sensing and cobamide biosynthesis related genes (345k)
- Additionalfile2.pdf
Additional file 2: Table. S2. as PDF Additional file 2: Table S2. Taxonomy information and relative abundance of keystone taxa in bacterial-fungal interaction (BFI), bacterial and fungal networks across the non-rhizosphere (Non), rhizosphere (Rhi), episphere (Epi) and endosphere (Endo) compartments (318k)
- Additionalfile3.pdf
Additional file 3: Table. S3. as PDF Additional file 3: Table S3. Key network topological characteristics for bacterial, fungal and bacterial-fungal interaction (BFI) networks across the non-rhizosphere (Non), rhizosphere (Rhi), episphere (Epi) and endosphere (Endo) compartments (228k)
- Additionalfile4.pdf
Additional file 4: Fig. S1. as PDF Additional file 4: Fig. S1. Highly connected modules within bacterial-fungal interaction (BFI) networks across the non-rhizosphere (Non), rhizosphere (Rhi), episphere (Epi) and endosphere (Endo) compartments (309k)
- Additionalfile5.pdf
Additional file 5: Fig. S2. as PDF Additional file 5: Fig. S2. Topological features of keystone taxa in (a) bacterial, (b) fungal and (c) bacterial-fungal interaction (BFI) networks across the non-rhizosphere (Non), rhizosphere (Rhi), episphere (Epi) and endosphere (Endo) compartments (343k)
- Additionalfile6.pdf
Additional file 6: Fig. S3. as PDF Additional file 6: Fig. S3. Degrees of keystone taxa from bacterial and fungal phyla in bacterial-fungal interaction (BFI) networks (322k)
- Additionalfile7.pdf
Additional file 7: Fig. S4. as PDF Additional file 7: Fig. S4. Bacterial community compositions across the non-rhizosphere (Non), rhizosphere (Rhi), episphere (Epi) and endosphere (Endo) compartments (333k)
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