One of the limitations of microsatellite markers is their obtaining, since it is necessary to have prior knowledge of the DNA sequence, to develop specific primer pairs flanking each identified microsatellite region  (Vieira et al., 2016). However, an alternative methodology is transferability, since microsatellite markers are potentially transferable to phylogenetically close species, due to the homologous nature of the DNA sequence in the flanking regions of the microsatellites, reducing the costs and long time to develop of this kind of markers  (Barbara et al., 2007).
In this work, microsatellite markers were obtained for all the seven Byrsonima species tested through transferability. Highlighting that of these seven Brazil native species, four are endemic species (B. intermedia, B. laxiflora, B. umbellata and B. viminifolia). The transferability of SSR markers developed for B. cydoniifolia in the species B. intermedia, B. laxiflora, B. verbascifolia, B. umbellata, B. subterranea, B. viminifolia and B. linearifolia showed high efficiency, varying from 11 loci transferred (64.8%) in B. viminifolia to 6 transferred loci (35.2%) in B. umbellata.
In Brazil, studies related to the development and use of microsatellite markers in plants are approximately two decades old and were first used in studies of forest species such as Ceiba pentandra  (Brondani et al.., 2003), Araucaria angustifolia  (Schmidt et al., 2007), and cultivated Oryza glumaepatula  (de Campos Vaz et al.., 2009). Several studies with microsatellite markers are also been developed for native Cerrado species Hymenaea courbaril  (Ciampi et al.. 2008), Tibouchina papyrus  (Telles et al.., 2011), Eugenia dysenterica  (Telles et al.., 2013), Byrsonima cydoniifolia  (Bernardes et al., 2014), Dipteryx alata  (Guimarães et al., 2019) Hymenaea stigonocarpa  (Gonçalves et al., 2019). Thus, this work increases the number of microsatellite markers developed for Cerrado biome species.
Similar results was found for another Byrsonima species by  Croft and Schaal (2012). Eight SSR markers were developed for the species Byrsonima crassifolia showing an average of 5.4 alleles/locus in four populations, ranging from two to 11 alleles. The heterozygosity observed at the loci among the four tested populations of B. crassifolia ranged from 0.000 to 0.933, while the genetic diversity ranged from 0.000 to 0.839. Here, considering the seven species, it is verified that the observed heterozygosity of the loci varied from the minimum value of 0.000 (B. umbellata – locus 09) to the maximum value of 1,000 (B. laxiflora – locus 09), while the genetic diversity varied from 0.063 (B. intermedia) to 0.924 (B. viminifolia).
In another study, they tested the transferability of ten markers developed for B. crassifolia in three other species of Byrsonima sp. The ten tested loci were transferred to B. pachyphylla (A = 3.6; HE = 0.481; HO = 0.322) and B. verbascifolia (A = 5.2; HE = 0.604; HO = 0.477), while only seven loci were transferred to B. coccolobifolia (A = 4.6; HE = 0.458; HO = 0.337)  (Menezes et al., 2014). In this study, the set of eight SSR loci developed to B. verbascifolia was capable to detect higher values of genetic variability (A = 5.875 HE = 0.706; Ho = 0.641) than values founded by  Menezes et al.. (2014) from the ten loci transferred from B. crassifolia to B. verbascifolia.
Thus, it is concluded that microsatellite marker panels transferred to the species B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. viminifolia and B. linearifolia are very informative, with a high combined probability of exclusion of paternity (Q ≥ 0.976) and the low combined probability of identity (I ≤ 9.91x10-6), so these markers are potentially suitable for future genetic-population studies. But, the set of markers transferred to B. umbellata is reasonably informative (Q = 0.778; I = 2.5x10-3), so it is necessary to develop more microsatellite markers for genetic-population studies for this species.
Considering phylogenetic studies, it is known that the genus Byrsonima is a monophyletic group within Malpighiaceae, confirmed by molecular and morphological synapomorphies. The genus Byrsonima is traditionally divided into two subgenera (Byrsonima subg. Byrsonima and Byrsonima subg. Macrozeugma Nied), initially proposed based on the stamen morphology  (Francener, 2016).
According to  Francener 2016, the subgenera can be separated based on the color of the petals, in addition to the morphology of the anther. In the subgenus B. subg. Byrsonima the posterior petal is yellow, and the connective does not surpass the anther locules or surpass only one-quarter of its size. While in B. subg. Macrozeugma the posterior petal is white or pink and the connective surpasses the locules in more than one-quarter of its size.
Among the species of Byrsonima sp. in this study, regarding the morphological characteristics, the species B. viminifolia, B. subterranea, B. intermedia, B. laxiflora, B. linearifolia presents terminal inflorescences with yellow colored petals and are included in the subgenera Byrsonima subg. Byrsonima, as also the specie B. cydoniifolia. While, B. umbellata is the only species, in this study, with white petals in the terminal inflorescences and belongs to the subgenera B. subg. Macrozeugma.
Therefore, the reason for the low number of microsatellites markers transferred to B. umbellata (six markers) compared to the other species (above 7 to 11 markers), is due to the lower efficiency in cross-amplification, probably because of the greater evolutionary distance of B. umbellata with B. cydoniifolia, being included in different subgenera. Once that the rate of successful amplification decreases as the genetic divergence between species increases  (Barbara et al., 2007).
According to  Mamede et al. (2018) when analyzing two species of Byrsonima belonging to different groups: Byrsonima coccolobifolia (Byrsonima subg. Macrozeugma) known as "murici-rosa". and Byrsonima crassifolia (L.) Kunth (Byrsonima subg. Byrsonima) commonly called “murici-Amarelo”, there was a set of 20 regions with high divergence, most of them intergenic sequences. Evidencing the genetic divergence between Byrsonima species belonging to the different subgenera.
Finally, the transferability technique for the development of microsatellite markers between different species of Byrsonima is effective and capable of generating numerous sets of microsatellite markers. This fact is fundamental and relevant for species of genus Byrsonima, Given the scope of this complex of species in Brazil, for which microsatellite markers have not yet been provided.
So, the markers developed in this study for Byrsonima species by transferability can provide relevant information in advanced studies in population-genetics for these species, once they are capable of detecting different magnitudes of genetic variability within and between populations, so can be used for identification of conservation units and for the investigation of genetic processes that occur in populations, such as the genetic diversity of populations, patterns of gene flow, the incidence of genetic drift and generation of the genetic neighborhood. So, different strategies for in situ or ex-situ conservation can be admitted and also for these species domestication, as a genetic resource.
Funding Work by V. Bernardes was supported by a fellowship from CAPES, and work by M. P. C. Telles, was also supported by productivity grants from CNPq. This study was partially supported by a Programa de Apoio a Núcleos de Excelência (PRONEX) grant from CNPq/FAPEG (AUX PESQ CH 007/2009) and by grants to the research network GENPAC (Geographical Genetics and Regional Planning for natural resources in Brazilian Cerrado) from CNPq/FAPEG (project no. 201110267000125 and 563839/ 2010-4–563973/2010-2). Our current research in Genetics and Genomics is developed in the context of National Institutes for Science and Technology (INCT) in Ecology, Evolution and Biodiversity Conservation, supported by MCTIC/CNpq (proc. 465610/2014-5) and FAPEG.