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Research article
Chengjiang Ruan
Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Institute of Plant Resources, Dalian Minzu University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8580-593X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Tea oil camellia (Camellia oleifera), an important woody oil tree, is a source of seed oil of high nutritional and medicinal values and has been widely planted in southern China. However, there are few reports on the identification of miRNAs involved in seed lipid metabolism in high- and low-oil cultivars of tea oil camellia.
Results An miRNA sequencing database was constructed for an Illumina platform, which was used to perform high-throughput small RNA sequencing of seeds of high- and low-oil cultivars of tea oil camellia at four different developmental stages, and the important relevant miRNAs and their target genes were identified. A total of 196 miRNAs, including 156 known miRNAs from 35 families and 40 novel miRNAs, were identified, and 55 significantly differentially expressed miRNAs were found. An integrated analysis of miRNA and mRNA transcriptome sequence data and qRT-PCR-based information was performed and revealed that 10 miRNA-mRNA function modules were related to lipid metabolism and 23 miRNA-mRNA function modules were involved in the regulation of seed size.
Conclusion Mining and studying the expression patterns and functions of miRNAs and their regulatory target genes can not only promote the development of miRNAs related to tea oil camellia in public resource databases but also provide important theoretical value and a scientific basis for the genetic improvement of new varieties of tea oil camellia in the future.
Keywords
tea oil camellia, small RNA sequencing, miRNA-mRNA function modules, lipid metabolism, seed size
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Chengjiang Ruan
Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Institute of Plant Resources, Dalian Minzu University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8580-593X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 17 Jul, 2019
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Physiology and Morphology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Tea oil camellia (Camellia oleifera), an important woody oil tree, is a source of seed oil of high nutritional and medicinal values and has been widely planted in southern China. However, there are few reports on the identification of miRNAs involved in seed lipid metabolism in high- and low-oil cultivars of tea oil camellia.
Results An miRNA sequencing database was constructed for an Illumina platform, which was used to perform high-throughput small RNA sequencing of seeds of high- and low-oil cultivars of tea oil camellia at four different developmental stages, and the important relevant miRNAs and their target genes were identified. A total of 196 miRNAs, including 156 known miRNAs from 35 families and 40 novel miRNAs, were identified, and 55 significantly differentially expressed miRNAs were found. An integrated analysis of miRNA and mRNA transcriptome sequence data and qRT-PCR-based information was performed and revealed that 10 miRNA-mRNA function modules were related to lipid metabolism and 23 miRNA-mRNA function modules were involved in the regulation of seed size.
Conclusion Mining and studying the expression patterns and functions of miRNAs and their regulatory target genes can not only promote the development of miRNAs related to tea oil camellia in public resource databases but also provide important theoretical value and a scientific basis for the genetic improvement of new varieties of tea oil camellia in the future.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
This is a list of supplementary files associated with this preprint. Click to download.
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