See the published version of this preprint at Journal of Gastrointestinal Cancer.
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Research Article
Ehsan Khalili
Tehran University of Medical Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9968-4374
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Purpose
Colorectal cancer (CRC) is the second leading cause of death from cancer in adults. Recent advances have shown that cancer cells can have some epigenetic changes involved in all stages of cancer. It has also been shown that miR-424 acts as gene expression regulators in many biological processes, including angiogenesis with mediators such as VEGF. In the current study, to identify the potential role of miR-424 in colorectal cancer progression, methylation status of miR-424 promoter region and its expression level have been evaluated. Besides, the correlation between VEGF level and miR-424 expression level has been assessed.
Methods
Methylation status miR-424 promoter was assessed using methylation-specific polymerase chain reaction (MSP). The expression level of miR-424 in human colorectal cancer tissue was analyzed by quantitative PCR. HCT116 cell line was selected to evaluate the correlation between the miR-424 expression level and the promoter's methylation status. VEGF expression, one out of mir-424 targets involved in angiogenesis and cancer progression, was measured by western blot analysis in the pairs of cancer tissues and their adjacent tissues.
Results
Our results have revealed that the promoter region of miR-424 is methylated in cancer cells compared to normal cells, leading to down-regulation of miR-424 in the colorectal cancer tissues compared to the normal tissues. Also, we found that the expression protein's level of VEGF in the tumor cells increased compared with normal tissues.
Conclusion
The present study suggests that hypermethylation downregulates miR-424. VEGF expression is upregulated with decreased miR-424 in colorectal cancer, which results in cancer progression.
Keywords
Colorectal cancer, miR-424, VEGF, Methylation
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CURRENT STATUS: Published
View the published article at Journal of Gastrointestinal Cancer.
Version 1
Posted 25 Feb, 2021
No community comments so far
Editorial decision: Minor revision
On 17 Feb, 2021
Reviews received
Received 14 Feb, 2021
Editor assigned
On 13 Feb, 2021
Reviewers invited
Invitations sent on 13 Feb, 2021
First submitted
On 13 Feb, 2021
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Subject Areas
Gastrointestinal Surgery
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A new preprint design is coming!
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See the published version of this preprint at Journal of Gastrointestinal Cancer.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Ehsan Khalili
Tehran University of Medical Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9968-4374
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Journal of Gastrointestinal Cancer.
Version 1
Posted 25 Feb, 2021
No community comments so far
Editorial decision: Minor revision
On 17 Feb, 2021
Reviews received
Received 14 Feb, 2021
Editor assigned
On 13 Feb, 2021
Reviewers invited
Invitations sent on 13 Feb, 2021
First submitted
On 13 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Gastrointestinal Surgery
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Journal of Gastrointestinal Cancer.
Purpose
Colorectal cancer (CRC) is the second leading cause of death from cancer in adults. Recent advances have shown that cancer cells can have some epigenetic changes involved in all stages of cancer. It has also been shown that miR-424 acts as gene expression regulators in many biological processes, including angiogenesis with mediators such as VEGF. In the current study, to identify the potential role of miR-424 in colorectal cancer progression, methylation status of miR-424 promoter region and its expression level have been evaluated. Besides, the correlation between VEGF level and miR-424 expression level has been assessed.
Methods
Methylation status miR-424 promoter was assessed using methylation-specific polymerase chain reaction (MSP). The expression level of miR-424 in human colorectal cancer tissue was analyzed by quantitative PCR. HCT116 cell line was selected to evaluate the correlation between the miR-424 expression level and the promoter's methylation status. VEGF expression, one out of mir-424 targets involved in angiogenesis and cancer progression, was measured by western blot analysis in the pairs of cancer tissues and their adjacent tissues.
Results
Our results have revealed that the promoter region of miR-424 is methylated in cancer cells compared to normal cells, leading to down-regulation of miR-424 in the colorectal cancer tissues compared to the normal tissues. Also, we found that the expression protein's level of VEGF in the tumor cells increased compared with normal tissues.
Conclusion
The present study suggests that hypermethylation downregulates miR-424. VEGF expression is upregulated with decreased miR-424 in colorectal cancer, which results in cancer progression.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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